CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/512.mrc Output diagnostic file name Micrographs_0601/512.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/512.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2426.000 8438.000 Mean, RMS : 4520.833 215.4955 TITLE 1: emi2mrc: 512.emi Mon Jan 06 16:53:37 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.04%, Obj 88.09%, Dif 73.67% TITLE 3: X 30.935, Y 483.656, Z -32.937, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02437 10200.00 10000.00 0.00 0.02566 10400.00 10000.00 0.00 0.02686 10600.00 10000.00 0.00 0.02862 10800.00 10000.00 0.00 0.03045 11000.00 10000.00 0.00 0.03247 11200.00 10000.00 0.00 0.03461 11400.00 10000.00 0.00 0.03668 11600.00 10000.00 0.00 0.03901 11800.00 10000.00 0.00 0.04143 12000.00 10000.00 0.00 0.04400 12200.00 10000.00 0.00 0.04682 12400.00 10000.00 0.00 0.04971 12600.00 10000.00 0.00 0.05264 12800.00 10000.00 0.00 0.05590 13000.00 10000.00 0.00 0.05927 13200.00 10000.00 0.00 0.06263 13400.00 10000.00 0.00 0.06623 13600.00 10000.00 0.00 0.07010 13800.00 10000.00 0.00 0.07436 14000.00 10000.00 0.00 0.07927 14200.00 10000.00 0.00 0.08436 14400.00 10000.00 0.00 0.08749 14600.00 10000.00 0.00 0.08832 14400.00 10200.00 0.00 0.08975 14600.00 10200.00 0.00 0.09050 14400.00 10400.00 0.00 0.09226 14600.00 10400.00 0.00 0.09290 14400.00 10600.00 0.00 0.09488 14600.00 10600.00 0.00 0.09539 14400.00 10800.00 0.00 0.09772 14600.00 10800.00 0.00 0.09811 14400.00 11000.00 0.00 0.10081 14600.00 11000.00 0.00 0.10109 14400.00 11200.00 0.00 0.10366 14600.00 11200.00 0.00 0.10380 14200.00 11400.00 0.00 0.10380 14400.00 11400.00 0.00 0.10676 14600.00 11400.00 0.00 0.10678 14200.00 11600.00 0.00 0.10705 14400.00 11600.00 0.00 0.11002 14200.00 11800.00 0.00 0.11069 14400.00 11800.00 0.00 0.11365 14200.00 12000.00 0.00 0.11446 14400.00 12000.00 0.00 0.11735 14200.00 12200.00 0.00 0.11812 14400.00 12200.00 0.00 0.12091 14200.00 12400.00 0.00 0.12193 14400.00 12400.00 0.00 0.12462 14200.00 12600.00 0.00 0.12591 14400.00 12600.00 0.00 0.12854 14200.00 12800.00 0.00 0.13043 14400.00 12800.00 0.00 0.13296 14200.00 13000.00 0.00 0.13477 14400.00 13000.00 0.00 0.13715 14200.00 13200.00 0.00 0.13963 14400.00 13200.00 0.00 0.14175 14200.00 13400.00 0.00 0.14471 14400.00 13400.00 0.00 0.14643 14200.00 13600.00 0.00 0.14999 14400.00 13600.00 0.00 0.15118 14200.00 13800.00 0.00 0.15516 14400.00 13800.00 0.00 0.15559 14200.00 14000.00 0.00 0.15983 14200.00 14200.00 0.00 0.16396 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14213.92 14262.76 14.48 0.16441 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/512.ctf NX, NY, NZ: 512 512 1 MODE : real