CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/511.mrc Output diagnostic file name Micrographs_0601/511.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/511.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2090.000 8178.000 Mean, RMS : 4520.789 217.9148 TITLE 1: emi2mrc: 511.emi Mon Jan 06 16:52:28 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.04%, Obj 88.09%, Dif 73.67% TITLE 3: X 29.141, Y 485.646, Z -32.937, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03505 10200.00 10000.00 0.00 0.03665 10400.00 10000.00 0.00 0.03814 10600.00 10000.00 0.00 0.04026 10800.00 10000.00 0.00 0.04238 11000.00 10000.00 0.00 0.04476 11200.00 10000.00 0.00 0.04735 11400.00 10000.00 0.00 0.04997 11600.00 10000.00 0.00 0.05285 11800.00 10000.00 0.00 0.05564 12000.00 10000.00 0.00 0.05863 12200.00 10000.00 0.00 0.06161 12400.00 10000.00 0.00 0.06466 12600.00 10000.00 0.00 0.06779 12800.00 10000.00 0.00 0.07117 13000.00 10000.00 0.00 0.07468 13200.00 10000.00 0.00 0.07837 13400.00 10000.00 0.00 0.08256 13600.00 10000.00 0.00 0.08682 13800.00 10000.00 0.00 0.09180 14000.00 10000.00 0.00 0.09623 14200.00 10000.00 0.00 0.09820 14400.00 10000.00 0.00 0.09830 14000.00 10200.00 0.00 0.09849 14200.00 10200.00 0.00 0.10046 14400.00 10200.00 0.00 0.10056 14000.00 10400.00 0.00 0.10104 14200.00 10400.00 0.00 0.10305 14400.00 10400.00 0.00 0.10313 14000.00 10600.00 0.00 0.10403 14200.00 10600.00 0.00 0.10605 14400.00 10600.00 0.00 0.10609 14000.00 10800.00 0.00 0.10707 14200.00 10800.00 0.00 0.10909 14000.00 11000.00 0.00 0.11025 14200.00 11000.00 0.00 0.11223 14000.00 11200.00 0.00 0.11343 14200.00 11200.00 0.00 0.11543 14000.00 11400.00 0.00 0.11718 14200.00 11400.00 0.00 0.11916 14000.00 11600.00 0.00 0.12102 14200.00 11600.00 0.00 0.12295 14000.00 11800.00 0.00 0.12507 14200.00 11800.00 0.00 0.12687 14000.00 12000.00 0.00 0.12920 14200.00 12000.00 0.00 0.13086 14000.00 12200.00 0.00 0.13333 14200.00 12200.00 0.00 0.13482 14000.00 12400.00 0.00 0.13780 14200.00 12400.00 0.00 0.13907 14000.00 12600.00 0.00 0.14221 14200.00 12600.00 0.00 0.14327 14000.00 12800.00 0.00 0.14717 14200.00 12800.00 0.00 0.14800 14000.00 13000.00 0.00 0.15201 14200.00 13000.00 0.00 0.15255 14000.00 13200.00 0.00 0.15732 14200.00 13200.00 0.00 0.15737 13800.00 13400.00 0.00 0.15792 14000.00 13400.00 0.00 0.16280 13800.00 13600.00 0.00 0.16411 14000.00 13600.00 0.00 0.16856 13800.00 13800.00 0.00 0.17146 14000.00 13800.00 0.00 0.17435 13800.00 14000.00 0.00 0.17517 14000.00 14000.00 0.00 0.17583 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13917.32 13982.99 12.54 0.17647 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/511.ctf NX, NY, NZ: 512 512 1 MODE : real