CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/509.mrc Output diagnostic file name Micrographs_0601/509.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/509.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2032.000 10304.00 Mean, RMS : 4443.066 210.2045 TITLE 1: emi2mrc: 509.emi Mon Jan 06 16:45:45 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 26.292, Y 490.007, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.12986 10200.00 10000.00 0.00 0.13724 10400.00 10000.00 0.00 0.14177 10600.00 10000.00 0.00 0.14304 10400.00 10200.00 0.00 0.14561 10600.00 10200.00 0.00 0.14624 10400.00 10400.00 0.00 0.14842 10400.00 10600.00 0.00 0.15052 10400.00 10600.00 5.00 0.15059 10400.00 10600.00 10.00 0.15063 10400.00 10600.00 15.00 0.15063 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10438.60 10626.30 13.34 0.15080 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/509.ctf NX, NY, NZ: 512 512 1 MODE : real