CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/508.mrc Output diagnostic file name Micrographs_0601/508.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/508.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2266.000 7870.000 Mean, RMS : 4443.949 212.6915 TITLE 1: emi2mrc: 508.emi Mon Jan 06 16:45:05 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 24.603, Y 492.236, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02767 10200.00 10000.00 0.00 0.02925 10400.00 10000.00 0.00 0.03094 10600.00 10000.00 0.00 0.03320 10800.00 10000.00 0.00 0.03542 11000.00 10000.00 0.00 0.03776 11200.00 10000.00 0.00 0.04025 11400.00 10000.00 0.00 0.04277 11600.00 10000.00 0.00 0.04562 11800.00 10000.00 0.00 0.04867 12000.00 10000.00 0.00 0.05192 12200.00 10000.00 0.00 0.05514 12400.00 10000.00 0.00 0.05847 12600.00 10000.00 0.00 0.06189 12800.00 10000.00 0.00 0.06560 13000.00 10000.00 0.00 0.06966 13200.00 10000.00 0.00 0.07372 13400.00 10000.00 0.00 0.07776 13600.00 10000.00 0.00 0.08191 13800.00 10000.00 0.00 0.08598 14000.00 10000.00 0.00 0.08876 14200.00 10000.00 0.00 0.09003 14000.00 10200.00 0.00 0.09100 14200.00 10200.00 0.00 0.09218 14000.00 10400.00 0.00 0.09335 14200.00 10400.00 0.00 0.09439 14000.00 10600.00 0.00 0.09605 14200.00 10600.00 0.00 0.09692 14000.00 10800.00 0.00 0.09874 14200.00 10800.00 0.00 0.09950 14000.00 11000.00 0.00 0.10175 14200.00 11000.00 0.00 0.10247 14000.00 11200.00 0.00 0.10470 14200.00 11200.00 0.00 0.10542 13800.00 11400.00 0.00 0.10553 14000.00 11400.00 0.00 0.10799 14200.00 11400.00 0.00 0.10866 13800.00 11600.00 0.00 0.10899 14000.00 11600.00 0.00 0.11143 14200.00 11600.00 0.00 0.11196 13800.00 11800.00 0.00 0.11257 14000.00 11800.00 0.00 0.11494 14200.00 11800.00 0.00 0.11531 13800.00 12000.00 0.00 0.11634 14000.00 12000.00 0.00 0.11867 14200.00 12000.00 0.00 0.11887 13800.00 12200.00 0.00 0.12031 14000.00 12200.00 0.00 0.12251 13800.00 12400.00 0.00 0.12444 14000.00 12400.00 0.00 0.12636 13800.00 12600.00 0.00 0.12813 14000.00 12600.00 0.00 0.12981 13800.00 12800.00 0.00 0.13213 14000.00 12800.00 0.00 0.13360 13800.00 13000.00 0.00 0.13601 14000.00 13000.00 0.00 0.13720 13800.00 13200.00 0.00 0.13999 14000.00 13200.00 0.00 0.14074 13800.00 13400.00 0.00 0.14369 14000.00 13400.00 0.00 0.14392 13800.00 13600.00 0.00 0.14737 13600.00 13800.00 0.00 0.14792 13800.00 13800.00 0.00 0.15068 13800.00 14000.00 0.00 0.15095 13800.00 14000.00 5.00 0.15098 13800.00 14000.00 10.00 0.15099 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13791.25 13914.45 10.00 0.15124 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/508.ctf NX, NY, NZ: 512 512 1 MODE : real