CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/506.mrc Output diagnostic file name Micrographs_0601/506.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/506.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2480.000 8832.000 Mean, RMS : 4422.771 214.6843 TITLE 1: emi2mrc: 506.emi Mon Jan 06 16:42:56 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.05%, Obj 88.11%, Dif 73.67% TITLE 3: X 21.433, Y 496.161, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01398 10200.00 10000.00 0.00 0.01484 10400.00 10000.00 0.00 0.01583 10600.00 10000.00 0.00 0.01692 10800.00 10000.00 0.00 0.01799 11000.00 10000.00 0.00 0.01930 11200.00 10000.00 0.00 0.02072 11400.00 10000.00 0.00 0.02225 11600.00 10000.00 0.00 0.02405 11800.00 10000.00 0.00 0.02588 12000.00 10000.00 0.00 0.02790 12200.00 10000.00 0.00 0.03004 12400.00 10000.00 0.00 0.03229 12600.00 10000.00 0.00 0.03458 12800.00 10000.00 0.00 0.03682 13000.00 10000.00 0.00 0.03921 13200.00 10000.00 0.00 0.04164 13400.00 10000.00 0.00 0.04434 13600.00 10000.00 0.00 0.04724 13800.00 10000.00 0.00 0.05001 14000.00 10000.00 0.00 0.05273 14200.00 10000.00 0.00 0.05561 14400.00 10000.00 0.00 0.05844 14600.00 10000.00 0.00 0.06149 14800.00 10000.00 0.00 0.06489 15000.00 10000.00 0.00 0.06826 15200.00 10000.00 0.00 0.07171 15400.00 10000.00 0.00 0.07448 15600.00 10000.00 0.00 0.07607 15800.00 10000.00 0.00 0.07670 15600.00 10200.00 0.00 0.07764 15800.00 10200.00 0.00 0.07824 15600.00 10400.00 0.00 0.07940 15800.00 10400.00 0.00 0.07998 15600.00 10600.00 0.00 0.08115 15800.00 10600.00 0.00 0.08171 15600.00 10800.00 0.00 0.08284 15800.00 10800.00 0.00 0.08342 15600.00 11000.00 0.00 0.08481 15800.00 11000.00 0.00 0.08538 15600.00 11200.00 0.00 0.08674 15800.00 11200.00 0.00 0.08726 15600.00 11400.00 0.00 0.08884 15800.00 11400.00 0.00 0.08930 15400.00 11600.00 0.00 0.08938 15600.00 11600.00 0.00 0.09093 15800.00 11600.00 0.00 0.09132 15400.00 11800.00 0.00 0.09181 15600.00 11800.00 0.00 0.09333 15800.00 11800.00 0.00 0.09369 15400.00 12000.00 0.00 0.09438 15600.00 12000.00 0.00 0.09590 15800.00 12000.00 0.00 0.09626 15400.00 12200.00 0.00 0.09690 15600.00 12200.00 0.00 0.09840 15800.00 12200.00 0.00 0.09868 15400.00 12400.00 0.00 0.09959 15600.00 12400.00 0.00 0.10104 15800.00 12400.00 0.00 0.10123 15400.00 12600.00 0.00 0.10227 15600.00 12600.00 0.00 0.10367 15800.00 12600.00 0.00 0.10378 15400.00 12800.00 0.00 0.10531 15600.00 12800.00 0.00 0.10666 15800.00 12800.00 0.00 0.10671 15400.00 13000.00 0.00 0.10833 15600.00 13000.00 0.00 0.10968 15400.00 13200.00 0.00 0.11150 15600.00 13200.00 0.00 0.11276 15400.00 13400.00 0.00 0.11457 15600.00 13400.00 0.00 0.11567 15400.00 13600.00 0.00 0.11759 15600.00 13600.00 0.00 0.11858 15400.00 13800.00 0.00 0.12063 15600.00 13800.00 0.00 0.12154 15400.00 14000.00 0.00 0.12349 15600.00 14000.00 0.00 0.12430 15400.00 14200.00 0.00 0.12665 15600.00 14200.00 0.00 0.12725 15200.00 14400.00 0.00 0.12737 15400.00 14400.00 0.00 0.12969 15600.00 14400.00 0.00 0.13001 15200.00 14600.00 0.00 0.13087 15400.00 14600.00 0.00 0.13294 15200.00 14800.00 0.00 0.13378 15400.00 14800.00 0.00 0.13558 15200.00 15000.00 0.00 0.13662 15400.00 15000.00 0.00 0.13805 15200.00 15200.00 0.00 0.13958 15400.00 15200.00 0.00 0.14030 15200.00 15400.00 0.00 0.14101 15400.00 15400.00 0.00 0.14131 15200.00 15600.00 0.00 0.14156 15200.00 15600.00 5.00 0.14168 15200.00 15600.00 10.00 0.14177 15200.00 15600.00 15.00 0.14182 15200.00 15600.00 20.00 0.14183 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15267.66 15565.20 16.11 0.14195 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/506.ctf NX, NY, NZ: 512 512 1 MODE : real