CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/504.mrc Output diagnostic file name Micrographs_0601/504.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/504.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2340.000 8220.000 Mean, RMS : 4331.239 213.4149 TITLE 1: emi2mrc: 504.emi Mon Jan 06 16:40:35 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.13%, Dif 73.67% TITLE 3: X 18.218, Y 500.307, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05458 10200.00 10000.00 0.00 0.05711 10400.00 10000.00 0.00 0.05932 10600.00 10000.00 0.00 0.06197 10800.00 10000.00 0.00 0.06440 11000.00 10000.00 0.00 0.06711 11200.00 10000.00 0.00 0.06988 11400.00 10000.00 0.00 0.07251 11600.00 10000.00 0.00 0.07533 11800.00 10000.00 0.00 0.07811 12000.00 10000.00 0.00 0.08125 12200.00 10000.00 0.00 0.08425 12400.00 10000.00 0.00 0.08708 12600.00 10000.00 0.00 0.08955 12800.00 10000.00 0.00 0.09153 13000.00 10000.00 0.00 0.09276 13200.00 10000.00 0.00 0.09329 13400.00 10000.00 0.00 0.09373 13600.00 10000.00 0.00 0.09379 12800.00 10200.00 0.00 0.09473 13000.00 10200.00 0.00 0.09589 13200.00 10200.00 0.00 0.09639 13400.00 10200.00 0.00 0.09681 12800.00 10400.00 0.00 0.09800 13000.00 10400.00 0.00 0.09911 13200.00 10400.00 0.00 0.09956 13400.00 10400.00 0.00 0.09989 12800.00 10600.00 0.00 0.10142 13000.00 10600.00 0.00 0.10253 13200.00 10600.00 0.00 0.10293 13400.00 10600.00 0.00 0.10317 12800.00 10800.00 0.00 0.10497 13000.00 10800.00 0.00 0.10610 13200.00 10800.00 0.00 0.10642 13400.00 10800.00 0.00 0.10656 12600.00 11000.00 0.00 0.10704 12800.00 11000.00 0.00 0.10891 13000.00 11000.00 0.00 0.10995 13200.00 11000.00 0.00 0.11009 13400.00 11000.00 0.00 0.11009 12600.00 11200.00 0.00 0.11079 12800.00 11200.00 0.00 0.11257 13000.00 11200.00 0.00 0.11341 12600.00 11400.00 0.00 0.11447 12800.00 11400.00 0.00 0.11610 13000.00 11400.00 0.00 0.11679 12600.00 11600.00 0.00 0.11792 12800.00 11600.00 0.00 0.11947 13000.00 11600.00 0.00 0.12008 12600.00 11800.00 0.00 0.12175 12800.00 11800.00 0.00 0.12318 13000.00 11800.00 0.00 0.12364 12400.00 12000.00 0.00 0.12365 12600.00 12000.00 0.00 0.12558 12800.00 12000.00 0.00 0.12681 13000.00 12000.00 0.00 0.12704 12400.00 12200.00 0.00 0.12739 12600.00 12200.00 0.00 0.12906 12800.00 12200.00 0.00 0.13004 12400.00 12400.00 0.00 0.13099 12600.00 12400.00 0.00 0.13211 12800.00 12400.00 0.00 0.13279 12600.00 12600.00 0.00 0.13330 12800.00 12600.00 0.00 0.13403 12600.00 12800.00 0.00 0.13449 12800.00 12800.00 0.00 0.13499 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12765.34 12825.76 -6.61 0.13503 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/504.ctf NX, NY, NZ: 512 512 1 MODE : real