CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/503.mrc Output diagnostic file name Micrographs_0601/503.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/503.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2152.000 10278.00 Mean, RMS : 4384.270 215.1398 TITLE 1: emi2mrc: 503.emi Mon Jan 06 16:39:05 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.13%, Dif 73.67% TITLE 3: X 16.619, Y 502.689, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02191 10200.00 10000.00 0.00 0.02316 10400.00 10000.00 0.00 0.02435 10600.00 10000.00 0.00 0.02597 10800.00 10000.00 0.00 0.02760 11000.00 10000.00 0.00 0.02947 11200.00 10000.00 0.00 0.03159 11400.00 10000.00 0.00 0.03388 11600.00 10000.00 0.00 0.03651 11800.00 10000.00 0.00 0.03912 12000.00 10000.00 0.00 0.04201 12200.00 10000.00 0.00 0.04490 12400.00 10000.00 0.00 0.04772 12600.00 10000.00 0.00 0.05082 12800.00 10000.00 0.00 0.05408 13000.00 10000.00 0.00 0.05746 13200.00 10000.00 0.00 0.06110 13400.00 10000.00 0.00 0.06496 13600.00 10000.00 0.00 0.06880 13800.00 10000.00 0.00 0.07272 14000.00 10000.00 0.00 0.07680 14200.00 10000.00 0.00 0.08082 14400.00 10000.00 0.00 0.08339 14600.00 10000.00 0.00 0.08417 14800.00 10000.00 0.00 0.08428 14400.00 10200.00 0.00 0.08568 14600.00 10200.00 0.00 0.08642 14800.00 10200.00 0.00 0.08648 14400.00 10400.00 0.00 0.08814 14600.00 10400.00 0.00 0.08886 14800.00 10400.00 0.00 0.08889 14400.00 10600.00 0.00 0.09077 14600.00 10600.00 0.00 0.09146 14400.00 10800.00 0.00 0.09338 14600.00 10800.00 0.00 0.09402 14400.00 11000.00 0.00 0.09626 14600.00 11000.00 0.00 0.09682 14400.00 11200.00 0.00 0.09911 14600.00 11200.00 0.00 0.09957 14200.00 11400.00 0.00 0.09973 14400.00 11400.00 0.00 0.10223 14600.00 11400.00 0.00 0.10259 14200.00 11600.00 0.00 0.10303 14400.00 11600.00 0.00 0.10548 14600.00 11600.00 0.00 0.10574 14200.00 11800.00 0.00 0.10644 14400.00 11800.00 0.00 0.10883 14600.00 11800.00 0.00 0.10900 14200.00 12000.00 0.00 0.10979 14400.00 12000.00 0.00 0.11215 14600.00 12000.00 0.00 0.11221 14200.00 12200.00 0.00 0.11332 14400.00 12200.00 0.00 0.11565 14200.00 12400.00 0.00 0.11718 14400.00 12400.00 0.00 0.11945 14200.00 12600.00 0.00 0.12089 14400.00 12600.00 0.00 0.12311 14200.00 12800.00 0.00 0.12483 14400.00 12800.00 0.00 0.12698 14200.00 13000.00 0.00 0.12869 14400.00 13000.00 0.00 0.13072 14200.00 13200.00 0.00 0.13306 14400.00 13200.00 0.00 0.13494 14200.00 13400.00 0.00 0.13761 14400.00 13400.00 0.00 0.13925 14200.00 13600.00 0.00 0.14238 14400.00 13600.00 0.00 0.14371 14200.00 13800.00 0.00 0.14711 14400.00 13800.00 0.00 0.14798 14200.00 14000.00 0.00 0.15129 14400.00 14000.00 0.00 0.15143 14000.00 14200.00 0.00 0.15192 14200.00 14200.00 0.00 0.15604 14200.00 14400.00 0.00 0.15713 14200.00 14400.00 5.00 0.15725 14200.00 14400.00 10.00 0.15732 14200.00 14400.00 15.00 0.15734 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14205.83 14336.26 13.02 0.15760 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/503.ctf NX, NY, NZ: 512 512 1 MODE : real