CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/502.mrc Output diagnostic file name Micrographs_0601/502.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/502.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1870.000 8106.000 Mean, RMS : 4394.754 216.3717 TITLE 1: emi2mrc: 502.emi Mon Jan 06 16:38:00 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.13%, Dif 73.67% TITLE 3: X 15.010, Y 504.916, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01883 10200.00 10000.00 0.00 0.02015 10400.00 10000.00 0.00 0.02159 10600.00 10000.00 0.00 0.02311 10800.00 10000.00 0.00 0.02443 11000.00 10000.00 0.00 0.02585 11200.00 10000.00 0.00 0.02742 11400.00 10000.00 0.00 0.02911 11600.00 10000.00 0.00 0.03098 11800.00 10000.00 0.00 0.03295 12000.00 10000.00 0.00 0.03514 12200.00 10000.00 0.00 0.03761 12400.00 10000.00 0.00 0.04033 12600.00 10000.00 0.00 0.04304 12800.00 10000.00 0.00 0.04594 13000.00 10000.00 0.00 0.04889 13200.00 10000.00 0.00 0.05156 13400.00 10000.00 0.00 0.05424 13600.00 10000.00 0.00 0.05717 13800.00 10000.00 0.00 0.06037 14000.00 10000.00 0.00 0.06367 14200.00 10000.00 0.00 0.06734 14400.00 10000.00 0.00 0.07104 14600.00 10000.00 0.00 0.07484 14800.00 10000.00 0.00 0.07840 15000.00 10000.00 0.00 0.08035 15200.00 10000.00 0.00 0.08101 15400.00 10000.00 0.00 0.08111 15000.00 10200.00 0.00 0.08228 15200.00 10200.00 0.00 0.08294 15400.00 10200.00 0.00 0.08299 15000.00 10400.00 0.00 0.08436 15200.00 10400.00 0.00 0.08500 15000.00 10600.00 0.00 0.08660 15200.00 10600.00 0.00 0.08719 15000.00 10800.00 0.00 0.08902 15200.00 10800.00 0.00 0.08955 14800.00 11000.00 0.00 0.08978 15000.00 11000.00 0.00 0.09173 15200.00 11000.00 0.00 0.09223 14800.00 11200.00 0.00 0.09237 15000.00 11200.00 0.00 0.09430 15200.00 11200.00 0.00 0.09477 14800.00 11400.00 0.00 0.09528 15000.00 11400.00 0.00 0.09721 15200.00 11400.00 0.00 0.09760 14800.00 11600.00 0.00 0.09820 15000.00 11600.00 0.00 0.10008 15200.00 11600.00 0.00 0.10037 14800.00 11800.00 0.00 0.10131 15000.00 11800.00 0.00 0.10316 15200.00 11800.00 0.00 0.10334 14800.00 12000.00 0.00 0.10463 15000.00 12000.00 0.00 0.10640 15200.00 12000.00 0.00 0.10644 14800.00 12200.00 0.00 0.10771 15000.00 12200.00 0.00 0.10938 14800.00 12400.00 0.00 0.11085 15000.00 12400.00 0.00 0.11251 14800.00 12600.00 0.00 0.11417 15000.00 12600.00 0.00 0.11584 14800.00 12800.00 0.00 0.11796 15000.00 12800.00 0.00 0.11953 14800.00 13000.00 0.00 0.12163 15000.00 13000.00 0.00 0.12309 14800.00 13200.00 0.00 0.12573 15000.00 13200.00 0.00 0.12708 14800.00 13400.00 0.00 0.12995 15000.00 13400.00 0.00 0.13114 14800.00 13600.00 0.00 0.13423 15000.00 13600.00 0.00 0.13520 14800.00 13800.00 0.00 0.13861 15000.00 13800.00 0.00 0.13934 14800.00 14000.00 0.00 0.14291 15000.00 14000.00 0.00 0.14333 14600.00 14200.00 0.00 0.14385 14800.00 14200.00 0.00 0.14781 14600.00 14400.00 0.00 0.14833 14800.00 14400.00 0.00 0.15227 14600.00 14600.00 0.00 0.15393 14800.00 14600.00 0.00 0.15742 14600.00 14800.00 0.00 0.15927 14800.00 14800.00 0.00 0.16119 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14738.51 14891.47 11.08 0.16174 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/502.ctf NX, NY, NZ: 512 512 1 MODE : real