CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/501.mrc Output diagnostic file name Micrographs_0601/501.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/501.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2430.000 8710.000 Mean, RMS : 4396.046 217.8716 TITLE 1: emi2mrc: 501.emi Mon Jan 06 16:37:15 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.13%, Dif 73.67% TITLE 3: X 13.463, Y 507.488, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01644 10200.00 10000.00 0.00 0.01742 10400.00 10000.00 0.00 0.01829 10600.00 10000.00 0.00 0.01935 10800.00 10000.00 0.00 0.02061 11000.00 10000.00 0.00 0.02211 11200.00 10000.00 0.00 0.02379 11400.00 10000.00 0.00 0.02565 11600.00 10000.00 0.00 0.02761 11800.00 10000.00 0.00 0.02974 12000.00 10000.00 0.00 0.03211 12200.00 10000.00 0.00 0.03448 12400.00 10000.00 0.00 0.03705 12600.00 10000.00 0.00 0.03966 12800.00 10000.00 0.00 0.04220 13000.00 10000.00 0.00 0.04477 13200.00 10000.00 0.00 0.04739 13400.00 10000.00 0.00 0.05000 13600.00 10000.00 0.00 0.05268 13800.00 10000.00 0.00 0.05568 14000.00 10000.00 0.00 0.05866 14200.00 10000.00 0.00 0.06183 14400.00 10000.00 0.00 0.06510 14600.00 10000.00 0.00 0.06836 14800.00 10000.00 0.00 0.07182 15000.00 10000.00 0.00 0.07522 15200.00 10000.00 0.00 0.07780 15400.00 10000.00 0.00 0.07869 15600.00 10000.00 0.00 0.07881 15200.00 10200.00 0.00 0.07933 15400.00 10200.00 0.00 0.08024 15600.00 10200.00 0.00 0.08041 15200.00 10400.00 0.00 0.08106 15400.00 10400.00 0.00 0.08201 15600.00 10400.00 0.00 0.08223 15800.00 10400.00 0.00 0.08225 15200.00 10600.00 0.00 0.08309 15400.00 10600.00 0.00 0.08408 15600.00 10600.00 0.00 0.08430 15200.00 10800.00 0.00 0.08514 15400.00 10800.00 0.00 0.08614 15600.00 10800.00 0.00 0.08633 15200.00 11000.00 0.00 0.08737 15400.00 11000.00 0.00 0.08835 15600.00 11000.00 0.00 0.08849 15200.00 11200.00 0.00 0.08959 15400.00 11200.00 0.00 0.09052 15600.00 11200.00 0.00 0.09062 15200.00 11400.00 0.00 0.09205 15400.00 11400.00 0.00 0.09294 15600.00 11400.00 0.00 0.09302 15200.00 11600.00 0.00 0.09470 15400.00 11600.00 0.00 0.09558 15600.00 11600.00 0.00 0.09563 15200.00 11800.00 0.00 0.09757 15400.00 11800.00 0.00 0.09841 15600.00 11800.00 0.00 0.09842 15200.00 12000.00 0.00 0.10045 15400.00 12000.00 0.00 0.10127 15200.00 12200.00 0.00 0.10361 15400.00 12200.00 0.00 0.10441 15200.00 12400.00 0.00 0.10718 15400.00 12400.00 0.00 0.10791 15200.00 12600.00 0.00 0.11047 15400.00 12600.00 0.00 0.11110 15200.00 12800.00 0.00 0.11375 15400.00 12800.00 0.00 0.11433 15200.00 13000.00 0.00 0.11708 15400.00 13000.00 0.00 0.11763 15000.00 13200.00 0.00 0.11801 15200.00 13200.00 0.00 0.12087 15400.00 13200.00 0.00 0.12129 15000.00 13400.00 0.00 0.12174 15200.00 13400.00 0.00 0.12454 15400.00 13400.00 0.00 0.12477 15000.00 13600.00 0.00 0.12537 15200.00 13600.00 0.00 0.12814 15400.00 13600.00 0.00 0.12819 15000.00 13800.00 0.00 0.12925 15200.00 13800.00 0.00 0.13199 15000.00 14000.00 0.00 0.13309 15200.00 14000.00 0.00 0.13575 15000.00 14200.00 0.00 0.13738 15200.00 14200.00 0.00 0.13992 15000.00 14400.00 0.00 0.14178 15200.00 14400.00 0.00 0.14415 15000.00 14600.00 0.00 0.14665 15200.00 14600.00 0.00 0.14867 15000.00 14800.00 0.00 0.15127 15200.00 14800.00 0.00 0.15287 15000.00 15000.00 0.00 0.15626 15200.00 15000.00 0.00 0.15698 15000.00 15200.00 0.00 0.15930 15000.00 15200.00 5.00 0.15931 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15000.00 15200.00 5.00 0.15931 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/501.ctf NX, NY, NZ: 512 512 1 MODE : real