CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/500.mrc Output diagnostic file name Micrographs_0601/500.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/500.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2288.000 8610.000 Mean, RMS : 4390.946 215.2913 TITLE 1: emi2mrc: 500.emi Mon Jan 06 16:36:21 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.13%, Dif 73.67% TITLE 3: X 12.745, Y 503.816, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02517 10200.00 10000.00 0.00 0.02662 10400.00 10000.00 0.00 0.02801 10600.00 10000.00 0.00 0.02979 10800.00 10000.00 0.00 0.03152 11000.00 10000.00 0.00 0.03345 11200.00 10000.00 0.00 0.03556 11400.00 10000.00 0.00 0.03757 11600.00 10000.00 0.00 0.03961 11800.00 10000.00 0.00 0.04178 12000.00 10000.00 0.00 0.04435 12200.00 10000.00 0.00 0.04718 12400.00 10000.00 0.00 0.05034 12600.00 10000.00 0.00 0.05363 12800.00 10000.00 0.00 0.05706 13000.00 10000.00 0.00 0.06044 13200.00 10000.00 0.00 0.06373 13400.00 10000.00 0.00 0.06733 13600.00 10000.00 0.00 0.07112 13800.00 10000.00 0.00 0.07523 14000.00 10000.00 0.00 0.07943 14200.00 10000.00 0.00 0.08340 14400.00 10000.00 0.00 0.08625 14600.00 10000.00 0.00 0.08746 14800.00 10000.00 0.00 0.08771 14400.00 10200.00 0.00 0.08867 14600.00 10200.00 0.00 0.08977 14800.00 10200.00 0.00 0.08992 14400.00 10400.00 0.00 0.09103 14600.00 10400.00 0.00 0.09202 14800.00 10400.00 0.00 0.09209 14400.00 10600.00 0.00 0.09351 14600.00 10600.00 0.00 0.09444 14800.00 10600.00 0.00 0.09447 14400.00 10800.00 0.00 0.09616 14600.00 10800.00 0.00 0.09702 14400.00 11000.00 0.00 0.09917 14600.00 11000.00 0.00 0.09997 14400.00 11200.00 0.00 0.10210 14600.00 11200.00 0.00 0.10284 14400.00 11400.00 0.00 0.10521 14600.00 11400.00 0.00 0.10583 14400.00 11600.00 0.00 0.10833 14600.00 11600.00 0.00 0.10879 14200.00 11800.00 0.00 0.10899 14400.00 11800.00 0.00 0.11155 14600.00 11800.00 0.00 0.11188 14200.00 12000.00 0.00 0.11241 14400.00 12000.00 0.00 0.11492 14600.00 12000.00 0.00 0.11515 14200.00 12200.00 0.00 0.11593 14400.00 12200.00 0.00 0.11840 14600.00 12200.00 0.00 0.11850 14200.00 12400.00 0.00 0.11977 14400.00 12400.00 0.00 0.12215 14200.00 12600.00 0.00 0.12366 14400.00 12600.00 0.00 0.12589 14200.00 12800.00 0.00 0.12777 14400.00 12800.00 0.00 0.12983 14200.00 13000.00 0.00 0.13155 14400.00 13000.00 0.00 0.13342 14200.00 13200.00 0.00 0.13558 14400.00 13200.00 0.00 0.13728 14200.00 13400.00 0.00 0.13988 14400.00 13400.00 0.00 0.14142 14200.00 13600.00 0.00 0.14461 14400.00 13600.00 0.00 0.14585 14200.00 13800.00 0.00 0.14937 14400.00 13800.00 0.00 0.15005 14200.00 14000.00 0.00 0.15358 14000.00 14200.00 0.00 0.15427 14200.00 14200.00 0.00 0.15768 14200.00 14400.00 0.00 0.15813 14200.00 14400.00 5.00 0.15821 14200.00 14400.00 10.00 0.15826 14200.00 14400.00 15.00 0.15828 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14212.71 14318.20 16.13 0.15872 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/500.ctf NX, NY, NZ: 512 512 1 MODE : real