CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/499.mrc Output diagnostic file name Micrographs_0601/499.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/499.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2446.000 8214.000 Mean, RMS : 4403.319 216.0752 TITLE 1: emi2mrc: 499.emi Mon Jan 06 16:35:19 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.13%, Dif 73.67% TITLE 3: X 14.208, Y 501.622, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02244 10200.00 10000.00 0.00 0.02363 10400.00 10000.00 0.00 0.02482 10600.00 10000.00 0.00 0.02655 10800.00 10000.00 0.00 0.02833 11000.00 10000.00 0.00 0.03025 11200.00 10000.00 0.00 0.03214 11400.00 10000.00 0.00 0.03421 11600.00 10000.00 0.00 0.03655 11800.00 10000.00 0.00 0.03896 12000.00 10000.00 0.00 0.04185 12200.00 10000.00 0.00 0.04462 12400.00 10000.00 0.00 0.04732 12600.00 10000.00 0.00 0.05008 12800.00 10000.00 0.00 0.05293 13000.00 10000.00 0.00 0.05607 13200.00 10000.00 0.00 0.05922 13400.00 10000.00 0.00 0.06258 13600.00 10000.00 0.00 0.06597 13800.00 10000.00 0.00 0.06947 14000.00 10000.00 0.00 0.07323 14200.00 10000.00 0.00 0.07733 14400.00 10000.00 0.00 0.08100 14600.00 10000.00 0.00 0.08311 14800.00 10000.00 0.00 0.08393 15000.00 10000.00 0.00 0.08415 14600.00 10200.00 0.00 0.08525 14800.00 10200.00 0.00 0.08603 15000.00 10200.00 0.00 0.08624 14600.00 10400.00 0.00 0.08742 14800.00 10400.00 0.00 0.08820 15000.00 10400.00 0.00 0.08840 14600.00 10600.00 0.00 0.08987 14800.00 10600.00 0.00 0.09065 15000.00 10600.00 0.00 0.09084 14600.00 10800.00 0.00 0.09229 14800.00 10800.00 0.00 0.09308 15000.00 10800.00 0.00 0.09324 14600.00 11000.00 0.00 0.09502 14800.00 11000.00 0.00 0.09587 15000.00 11000.00 0.00 0.09601 14600.00 11200.00 0.00 0.09785 14800.00 11200.00 0.00 0.09875 15000.00 11200.00 0.00 0.09876 14600.00 11400.00 0.00 0.10090 14800.00 11400.00 0.00 0.10171 14400.00 11600.00 0.00 0.10186 14600.00 11600.00 0.00 0.10402 14800.00 11600.00 0.00 0.10471 14400.00 11800.00 0.00 0.10523 14600.00 11800.00 0.00 0.10740 14800.00 11800.00 0.00 0.10796 14400.00 12000.00 0.00 0.10881 14600.00 12000.00 0.00 0.11093 14800.00 12000.00 0.00 0.11136 14400.00 12200.00 0.00 0.11250 14600.00 12200.00 0.00 0.11454 14800.00 12200.00 0.00 0.11484 14400.00 12400.00 0.00 0.11651 14600.00 12400.00 0.00 0.11845 14800.00 12400.00 0.00 0.11863 14400.00 12600.00 0.00 0.12050 14600.00 12600.00 0.00 0.12231 14800.00 12600.00 0.00 0.12232 14400.00 12800.00 0.00 0.12486 14600.00 12800.00 0.00 0.12649 14400.00 13000.00 0.00 0.12910 14600.00 13000.00 0.00 0.13053 14400.00 13200.00 0.00 0.13346 14600.00 13200.00 0.00 0.13464 14400.00 13400.00 0.00 0.13792 14600.00 13400.00 0.00 0.13883 14400.00 13600.00 0.00 0.14269 14600.00 13600.00 0.00 0.14323 14200.00 13800.00 0.00 0.14384 14400.00 13800.00 0.00 0.14758 14600.00 13800.00 0.00 0.14762 14200.00 14000.00 0.00 0.14864 14400.00 14000.00 0.00 0.15212 14200.00 14200.00 0.00 0.15422 14400.00 14200.00 0.00 0.15686 14200.00 14400.00 0.00 0.15805 14400.00 14400.00 0.00 0.15912 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14305.80 14496.57 12.73 0.15967 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/499.ctf NX, NY, NZ: 512 512 1 MODE : real