CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/498.mrc Output diagnostic file name Micrographs_0601/498.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/498.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2304.000 8156.000 Mean, RMS : 4453.662 219.7090 TITLE 1: emi2mrc: 498.emi Mon Jan 06 16:33:54 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 17.484, Y 497.204, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02444 13800.00 10000.00 0.00 -0.02437 14000.00 10000.00 0.00 -0.02411 14200.00 10000.00 0.00 -0.02407 14600.00 10000.00 0.00 -0.02395 14800.00 10000.00 0.00 -0.02372 15000.00 10000.00 0.00 -0.02341 15200.00 10000.00 0.00 -0.02313 15400.00 10000.00 0.00 -0.02283 15600.00 10000.00 0.00 -0.02243 15800.00 10000.00 0.00 -0.02196 16000.00 10000.00 0.00 -0.02156 16200.00 10000.00 0.00 -0.02113 16400.00 10000.00 0.00 -0.02064 16600.00 10000.00 0.00 -0.02011 16800.00 10000.00 0.00 -0.01940 17000.00 10000.00 0.00 -0.01868 17200.00 10000.00 0.00 -0.01797 17400.00 10000.00 0.00 -0.01727 17600.00 10000.00 0.00 -0.01656 17800.00 10000.00 0.00 -0.01571 18000.00 10000.00 0.00 -0.01485 18200.00 10000.00 0.00 -0.01406 18400.00 10000.00 0.00 -0.01332 18600.00 10000.00 0.00 -0.01254 18800.00 10000.00 0.00 -0.01169 19000.00 10000.00 0.00 -0.01091 19200.00 10000.00 0.00 -0.01009 19400.00 10000.00 0.00 -0.00926 19600.00 10000.00 0.00 -0.00844 19800.00 10000.00 0.00 -0.00742 20000.00 10000.00 0.00 -0.00631 20200.00 10000.00 0.00 -0.00527 20400.00 10000.00 0.00 -0.00430 20600.00 10000.00 0.00 -0.00344 20800.00 10000.00 0.00 -0.00247 21000.00 10000.00 0.00 -0.00147 21200.00 10000.00 0.00 -0.00061 21400.00 10000.00 0.00 0.00036 21600.00 10000.00 0.00 0.00137 21800.00 10000.00 0.00 0.00228 22000.00 10000.00 0.00 0.00346 22200.00 10000.00 0.00 0.00472 22400.00 10000.00 0.00 0.00594 22600.00 10000.00 0.00 0.00742 22800.00 10000.00 0.00 0.00889 23000.00 10000.00 0.00 0.01024 23200.00 10000.00 0.00 0.01165 23400.00 10000.00 0.00 0.01307 23600.00 10000.00 0.00 0.01450 23800.00 10000.00 0.00 0.01601 24000.00 10000.00 0.00 0.01755 24200.00 10000.00 0.00 0.01901 24400.00 10000.00 0.00 0.02047 24600.00 10000.00 0.00 0.02169 24800.00 10000.00 0.00 0.02262 25000.00 10000.00 0.00 0.02346 25200.00 10000.00 0.00 0.02414 25400.00 10000.00 0.00 0.02457 25600.00 10000.00 0.00 0.02472 25800.00 10000.00 0.00 0.02475 26000.00 10000.00 0.00 0.02481 25400.00 10200.00 0.00 0.02504 25600.00 10200.00 0.00 0.02523 25800.00 10200.00 0.00 0.02526 26000.00 10200.00 0.00 0.02530 25400.00 10400.00 0.00 0.02562 25600.00 10400.00 0.00 0.02581 26000.00 10400.00 0.00 0.02582 25400.00 10600.00 0.00 0.02607 25600.00 10600.00 0.00 0.02622 25400.00 10800.00 0.00 0.02656 25600.00 10800.00 0.00 0.02663 25400.00 11000.00 0.00 0.02696 25600.00 11000.00 0.00 0.02698 25400.00 11200.00 0.00 0.02719 25600.00 11200.00 0.00 0.02720 25400.00 11400.00 0.00 0.02755 25600.00 11400.00 0.00 0.02757 25400.00 11600.00 0.00 0.02789 25600.00 11600.00 0.00 0.02790 25400.00 11800.00 0.00 0.02814 25600.00 11800.00 0.00 0.02817 25400.00 12000.00 0.00 0.02849 25200.00 12200.00 0.00 0.02861 25400.00 12200.00 0.00 0.02890 25200.00 12400.00 0.00 0.02896 25400.00 12400.00 0.00 0.02921 25200.00 12600.00 0.00 0.02932 25400.00 12600.00 0.00 0.02957 25600.00 12600.00 0.00 0.02962 25800.00 12600.00 0.00 0.02965 25200.00 12800.00 0.00 0.02992 25400.00 12800.00 0.00 0.03019 25600.00 12800.00 0.00 0.03027 25200.00 13000.00 0.00 0.03057 25400.00 13000.00 0.00 0.03085 25600.00 13000.00 0.00 0.03091 25200.00 13200.00 0.00 0.03115 25400.00 13200.00 0.00 0.03142 25600.00 13200.00 0.00 0.03143 25200.00 13400.00 0.00 0.03169 25400.00 13400.00 0.00 0.03191 25200.00 13600.00 0.00 0.03229 25400.00 13600.00 0.00 0.03252 25600.00 13600.00 0.00 0.03254 25200.00 13800.00 0.00 0.03304 25400.00 13800.00 0.00 0.03330 25600.00 13800.00 0.00 0.03337 25200.00 14000.00 0.00 0.03391 25400.00 14000.00 0.00 0.03419 25600.00 14000.00 0.00 0.03426 25200.00 14200.00 0.00 0.03476 25400.00 14200.00 0.00 0.03505 25600.00 14200.00 0.00 0.03508 25200.00 14400.00 0.00 0.03543 25400.00 14400.00 0.00 0.03575 25600.00 14400.00 0.00 0.03575 25200.00 14600.00 0.00 0.03618 25400.00 14600.00 0.00 0.03649 25200.00 14800.00 0.00 0.03698 25400.00 14800.00 0.00 0.03721 25200.00 15000.00 0.00 0.03774 25400.00 15000.00 0.00 0.03789 25000.00 15200.00 0.00 0.03789 25200.00 15200.00 0.00 0.03853 25400.00 15200.00 0.00 0.03863 25000.00 15400.00 0.00 0.03877 25200.00 15400.00 0.00 0.03935 25400.00 15400.00 0.00 0.03942 25000.00 15600.00 0.00 0.03957 25200.00 15600.00 0.00 0.04011 25400.00 15600.00 0.00 0.04018 25000.00 15800.00 0.00 0.04042 25200.00 15800.00 0.00 0.04094 25400.00 15800.00 0.00 0.04104 25000.00 16000.00 0.00 0.04141 25200.00 16000.00 0.00 0.04188 25400.00 16000.00 0.00 0.04195 25000.00 16200.00 0.00 0.04234 25200.00 16200.00 0.00 0.04277 25400.00 16200.00 0.00 0.04284 25000.00 16400.00 0.00 0.04337 25200.00 16400.00 0.00 0.04378 25400.00 16400.00 0.00 0.04389 25600.00 16400.00 0.00 0.04392 25000.00 16600.00 0.00 0.04446 25200.00 16600.00 0.00 0.04485 25400.00 16600.00 0.00 0.04499 25600.00 16600.00 0.00 0.04503 25000.00 16800.00 0.00 0.04555 25200.00 16800.00 0.00 0.04593 25400.00 16800.00 0.00 0.04611 25600.00 16800.00 0.00 0.04615 25000.00 17000.00 0.00 0.04655 25200.00 17000.00 0.00 0.04697 25400.00 17000.00 0.00 0.04719 25600.00 17000.00 0.00 0.04720 25000.00 17200.00 0.00 0.04751 25200.00 17200.00 0.00 0.04796 25400.00 17200.00 0.00 0.04817 25000.00 17400.00 0.00 0.04864 25200.00 17400.00 0.00 0.04908 25400.00 17400.00 0.00 0.04927 25000.00 17600.00 0.00 0.04977 25200.00 17600.00 0.00 0.05021 25400.00 17600.00 0.00 0.05041 25000.00 17800.00 0.00 0.05095 25200.00 17800.00 0.00 0.05143 25400.00 17800.00 0.00 0.05164 25000.00 18000.00 0.00 0.05217 25200.00 18000.00 0.00 0.05268 25400.00 18000.00 0.00 0.05282 25000.00 18200.00 0.00 0.05341 25200.00 18200.00 0.00 0.05388 25400.00 18200.00 0.00 0.05395 25000.00 18400.00 0.00 0.05476 25200.00 18400.00 0.00 0.05519 25400.00 18400.00 0.00 0.05525 24800.00 18600.00 0.00 0.05536 25000.00 18600.00 0.00 0.05625 25200.00 18600.00 0.00 0.05665 25400.00 18600.00 0.00 0.05671 24800.00 18800.00 0.00 0.05685 25000.00 18800.00 0.00 0.05769 25200.00 18800.00 0.00 0.05805 25400.00 18800.00 0.00 0.05808 24800.00 19000.00 0.00 0.05811 25000.00 19000.00 0.00 0.05891 25200.00 19000.00 0.00 0.05926 25400.00 19000.00 0.00 0.05931 24800.00 19200.00 0.00 0.05942 25000.00 19200.00 0.00 0.06024 25200.00 19200.00 0.00 0.06063 25400.00 19200.00 0.00 0.06070 24800.00 19400.00 0.00 0.06081 25000.00 19400.00 0.00 0.06165 25200.00 19400.00 0.00 0.06203 25400.00 19400.00 0.00 0.06204 24800.00 19600.00 0.00 0.06223 25000.00 19600.00 0.00 0.06304 25200.00 19600.00 0.00 0.06340 24800.00 19800.00 0.00 0.06361 25000.00 19800.00 0.00 0.06442 25200.00 19800.00 0.00 0.06479 24800.00 20000.00 0.00 0.06505 25000.00 20000.00 0.00 0.06586 25200.00 20000.00 0.00 0.06622 24800.00 20200.00 0.00 0.06657 25000.00 20200.00 0.00 0.06737 25200.00 20200.00 0.00 0.06770 24800.00 20400.00 0.00 0.06810 25000.00 20400.00 0.00 0.06887 25200.00 20400.00 0.00 0.06916 24800.00 20600.00 0.00 0.06963 25000.00 20600.00 0.00 0.07037 25200.00 20600.00 0.00 0.07061 24800.00 20800.00 0.00 0.07104 25000.00 20800.00 0.00 0.07176 25200.00 20800.00 0.00 0.07195 24800.00 21000.00 0.00 0.07261 25000.00 21000.00 0.00 0.07329 25200.00 21000.00 0.00 0.07340 24800.00 21200.00 0.00 0.07417 25000.00 21200.00 0.00 0.07478 25200.00 21200.00 0.00 0.07478 24800.00 21400.00 0.00 0.07569 25000.00 21400.00 0.00 0.07623 24800.00 21600.00 0.00 0.07724 25000.00 21600.00 0.00 0.07772 24800.00 21800.00 0.00 0.07880 25000.00 21800.00 0.00 0.07919 24600.00 22000.00 0.00 0.07936 24800.00 22000.00 0.00 0.08048 25000.00 22000.00 0.00 0.08079 24600.00 22200.00 0.00 0.08095 24800.00 22200.00 0.00 0.08199 25000.00 22200.00 0.00 0.08227 24600.00 22400.00 0.00 0.08261 24800.00 22400.00 0.00 0.08361 25000.00 22400.00 0.00 0.08386 24600.00 22600.00 0.00 0.08435 24800.00 22600.00 0.00 0.08532 25000.00 22600.00 0.00 0.08553 24600.00 22800.00 0.00 0.08607 24800.00 22800.00 0.00 0.08703 25000.00 22800.00 0.00 0.08720 24600.00 23000.00 0.00 0.08792 24800.00 23000.00 0.00 0.08887 25000.00 23000.00 0.00 0.08897 24600.00 23200.00 0.00 0.08998 24800.00 23200.00 0.00 0.09088 25000.00 23200.00 0.00 0.09089 24600.00 23400.00 0.00 0.09202 24800.00 23400.00 0.00 0.09286 24600.00 23600.00 0.00 0.09388 24800.00 23600.00 0.00 0.09465 24600.00 23800.00 0.00 0.09579 24800.00 23800.00 0.00 0.09648 24600.00 24000.00 0.00 0.09758 24800.00 24000.00 0.00 0.09815 24400.00 24200.00 0.00 0.09836 24600.00 24200.00 0.00 0.09960 24800.00 24200.00 0.00 0.09995 24400.00 24400.00 0.00 0.10027 24600.00 24400.00 0.00 0.10125 24800.00 24400.00 0.00 0.10133 24600.00 24600.00 0.00 0.10206 24400.00 24800.00 35.00 0.10211 24400.00 24800.00 40.00 0.10215 24400.00 24800.00 45.00 0.10216 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24523.11 24718.86 45.00 0.10231 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/498.ctf NX, NY, NZ: 512 512 1 MODE : real