CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/495.mrc Output diagnostic file name Micrographs_0601/495.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/495.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2366.000 9326.000 Mean, RMS : 4408.250 216.8322 TITLE 1: emi2mrc: 495.emi Mon Jan 06 16:30:44 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 22.091, Y 490.438, Z -32.615, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02320 10200.00 10000.00 0.00 0.02447 10400.00 10000.00 0.00 0.02565 10600.00 10000.00 0.00 0.02720 10800.00 10000.00 0.00 0.02877 11000.00 10000.00 0.00 0.03054 11200.00 10000.00 0.00 0.03241 11400.00 10000.00 0.00 0.03430 11600.00 10000.00 0.00 0.03642 11800.00 10000.00 0.00 0.03854 12000.00 10000.00 0.00 0.04088 12200.00 10000.00 0.00 0.04314 12400.00 10000.00 0.00 0.04544 12600.00 10000.00 0.00 0.04800 12800.00 10000.00 0.00 0.05073 13000.00 10000.00 0.00 0.05354 13200.00 10000.00 0.00 0.05643 13400.00 10000.00 0.00 0.05944 13600.00 10000.00 0.00 0.06231 13800.00 10000.00 0.00 0.06541 14000.00 10000.00 0.00 0.06887 14200.00 10000.00 0.00 0.07233 14400.00 10000.00 0.00 0.07561 14600.00 10000.00 0.00 0.07846 14800.00 10000.00 0.00 0.08041 15000.00 10000.00 0.00 0.08132 15200.00 10000.00 0.00 0.08151 14800.00 10200.00 0.00 0.08222 15000.00 10200.00 0.00 0.08309 15200.00 10200.00 0.00 0.08325 14800.00 10400.00 0.00 0.08408 15000.00 10400.00 0.00 0.08489 15200.00 10400.00 0.00 0.08502 14800.00 10600.00 0.00 0.08599 15000.00 10600.00 0.00 0.08676 15200.00 10600.00 0.00 0.08687 14800.00 10800.00 0.00 0.08815 15000.00 10800.00 0.00 0.08893 15200.00 10800.00 0.00 0.08907 14800.00 11000.00 0.00 0.09060 15000.00 11000.00 0.00 0.09142 15200.00 11000.00 0.00 0.09156 14800.00 11200.00 0.00 0.09307 15000.00 11200.00 0.00 0.09388 15200.00 11200.00 0.00 0.09398 14800.00 11400.00 0.00 0.09589 15000.00 11400.00 0.00 0.09664 14800.00 11600.00 0.00 0.09862 15000.00 11600.00 0.00 0.09925 14600.00 11800.00 0.00 0.09952 14800.00 11800.00 0.00 0.10143 15000.00 11800.00 0.00 0.10196 14600.00 12000.00 0.00 0.10243 14800.00 12000.00 0.00 0.10429 15000.00 12000.00 0.00 0.10473 14600.00 12200.00 0.00 0.10543 14800.00 12200.00 0.00 0.10723 15000.00 12200.00 0.00 0.10757 14600.00 12400.00 0.00 0.10865 14800.00 12400.00 0.00 0.11038 15000.00 12400.00 0.00 0.11060 14600.00 12600.00 0.00 0.11183 14800.00 12600.00 0.00 0.11348 15000.00 12600.00 0.00 0.11356 14600.00 12800.00 0.00 0.11532 14800.00 12800.00 0.00 0.11686 14600.00 13000.00 0.00 0.11846 14800.00 13000.00 0.00 0.11990 14600.00 13200.00 0.00 0.12180 14800.00 13200.00 0.00 0.12316 14600.00 13400.00 0.00 0.12524 14800.00 13400.00 0.00 0.12647 14600.00 13600.00 0.00 0.12883 14800.00 13600.00 0.00 0.12990 14400.00 13800.00 0.00 0.13014 14600.00 13800.00 0.00 0.13242 14800.00 13800.00 0.00 0.13322 14400.00 14000.00 0.00 0.13336 14600.00 14000.00 0.00 0.13541 14800.00 14000.00 0.00 0.13590 14400.00 14200.00 0.00 0.13684 14600.00 14200.00 0.00 0.13852 14800.00 14200.00 0.00 0.13867 14400.00 14400.00 0.00 0.13904 14600.00 14400.00 0.00 0.14060 14400.00 14600.00 0.00 0.14103 14600.00 14600.00 0.00 0.14251 14600.00 14800.00 0.00 0.14320 14800.00 14600.00 85.00 0.14320 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14749.85 14619.31 87.93 0.14331 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/495.ctf NX, NY, NZ: 512 512 1 MODE : real