CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/494.mrc Output diagnostic file name Micrographs_0601/494.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/494.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2234.000 8388.000 Mean, RMS : 4481.079 218.4817 TITLE 1: emi2mrc: 494.emi Mon Jan 06 16:28:20 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.08%, Dif 73.67% TITLE 3: X 25.325, Y 486.803, Z -32.497, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00678 10200.00 10000.00 0.00 0.00752 10400.00 10000.00 0.00 0.00826 10600.00 10000.00 0.00 0.00914 10800.00 10000.00 0.00 0.01018 11000.00 10000.00 0.00 0.01120 11200.00 10000.00 0.00 0.01230 11400.00 10000.00 0.00 0.01352 11600.00 10000.00 0.00 0.01489 11800.00 10000.00 0.00 0.01617 12000.00 10000.00 0.00 0.01732 12200.00 10000.00 0.00 0.01878 12400.00 10000.00 0.00 0.02043 12600.00 10000.00 0.00 0.02201 12800.00 10000.00 0.00 0.02386 13000.00 10000.00 0.00 0.02573 13200.00 10000.00 0.00 0.02751 13400.00 10000.00 0.00 0.02941 13600.00 10000.00 0.00 0.03122 13800.00 10000.00 0.00 0.03301 14000.00 10000.00 0.00 0.03493 14200.00 10000.00 0.00 0.03712 14400.00 10000.00 0.00 0.03934 14600.00 10000.00 0.00 0.04146 14800.00 10000.00 0.00 0.04375 15000.00 10000.00 0.00 0.04631 15200.00 10000.00 0.00 0.04918 15400.00 10000.00 0.00 0.05218 15600.00 10000.00 0.00 0.05539 15800.00 10000.00 0.00 0.05875 16000.00 10000.00 0.00 0.06203 16200.00 10000.00 0.00 0.06518 16400.00 10000.00 0.00 0.06756 16600.00 10000.00 0.00 0.06873 16800.00 10000.00 0.00 0.06914 17000.00 10000.00 0.00 0.06925 16600.00 10200.00 0.00 0.07025 16800.00 10200.00 0.00 0.07060 17000.00 10200.00 0.00 0.07065 16600.00 10400.00 0.00 0.07171 16800.00 10400.00 0.00 0.07202 16400.00 10600.00 0.00 0.07228 16600.00 10600.00 0.00 0.07337 16800.00 10600.00 0.00 0.07366 16400.00 10800.00 0.00 0.07409 16600.00 10800.00 0.00 0.07518 16800.00 10800.00 0.00 0.07541 16400.00 11000.00 0.00 0.07592 16600.00 11000.00 0.00 0.07695 16800.00 11000.00 0.00 0.07706 16400.00 11200.00 0.00 0.07761 16600.00 11200.00 0.00 0.07852 16800.00 11200.00 0.00 0.07857 16400.00 11400.00 0.00 0.07930 16600.00 11400.00 0.00 0.08017 16800.00 11400.00 0.00 0.08023 16400.00 11600.00 0.00 0.08111 16600.00 11600.00 0.00 0.08201 16800.00 11600.00 0.00 0.08212 16400.00 11800.00 0.00 0.08321 16600.00 11800.00 0.00 0.08414 16800.00 11800.00 0.00 0.08420 16400.00 12000.00 0.00 0.08539 16600.00 12000.00 0.00 0.08627 16400.00 12200.00 0.00 0.08755 16600.00 12200.00 0.00 0.08834 16400.00 12400.00 0.00 0.08987 16600.00 12400.00 0.00 0.09057 16400.00 12600.00 0.00 0.09213 16600.00 12600.00 0.00 0.09274 16400.00 12800.00 0.00 0.09431 16600.00 12800.00 0.00 0.09485 16400.00 13000.00 0.00 0.09661 16600.00 13000.00 0.00 0.09714 16200.00 13200.00 0.00 0.09721 16400.00 13200.00 0.00 0.09935 16600.00 13200.00 0.00 0.09984 16200.00 13400.00 0.00 0.09994 16400.00 13400.00 0.00 0.10204 16600.00 13400.00 0.00 0.10247 16200.00 13600.00 0.00 0.10289 16400.00 13600.00 0.00 0.10496 16600.00 13600.00 0.00 0.10538 16200.00 13800.00 0.00 0.10619 16400.00 13800.00 0.00 0.10826 16600.00 13800.00 0.00 0.10863 16200.00 14000.00 0.00 0.10940 16400.00 14000.00 0.00 0.11142 16600.00 14000.00 0.00 0.11167 16200.00 14200.00 0.00 0.11271 16400.00 14200.00 0.00 0.11462 16600.00 14200.00 0.00 0.11473 16200.00 14400.00 0.00 0.11590 16400.00 14400.00 0.00 0.11775 16200.00 14600.00 0.00 0.11932 16400.00 14600.00 0.00 0.12108 16200.00 14800.00 0.00 0.12273 16400.00 14800.00 0.00 0.12439 16200.00 15000.00 0.00 0.12629 16400.00 15000.00 0.00 0.12779 16200.00 15200.00 0.00 0.12986 16400.00 15200.00 0.00 0.13118 16200.00 15400.00 0.00 0.13361 16400.00 15400.00 0.00 0.13472 16200.00 15600.00 0.00 0.13778 16400.00 15600.00 0.00 0.13857 16200.00 15800.00 0.00 0.14182 16400.00 15800.00 0.00 0.14219 16000.00 16000.00 0.00 0.14313 16200.00 16000.00 0.00 0.14629 16000.00 16200.00 0.00 0.14694 16200.00 16200.00 0.00 0.14894 16200.00 16400.00 15.00 0.14902 16200.00 16400.00 20.00 0.14908 16200.00 16400.00 25.00 0.14910 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16143.94 16344.14 25.00 0.14947 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/494.ctf NX, NY, NZ: 512 512 1 MODE : real