CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/493.mrc Output diagnostic file name Micrographs_0601/493.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/493.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2224.000 8402.000 Mean, RMS : 4512.764 219.1876 TITLE 1: emi2mrc: 493.emi Mon Jan 06 16:25:38 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 30.180, Y 480.024, Z -32.497, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01015 11400.00 10000.00 0.00 -0.00990 11600.00 10000.00 0.00 -0.00957 11800.00 10000.00 0.00 -0.00913 12000.00 10000.00 0.00 -0.00871 12200.00 10000.00 0.00 -0.00824 12400.00 10000.00 0.00 -0.00775 12600.00 10000.00 0.00 -0.00731 12800.00 10000.00 0.00 -0.00685 13000.00 10000.00 0.00 -0.00628 13200.00 10000.00 0.00 -0.00557 13400.00 10000.00 0.00 -0.00483 13600.00 10000.00 0.00 -0.00423 13800.00 10000.00 0.00 -0.00369 14000.00 10000.00 0.00 -0.00310 14200.00 10000.00 0.00 -0.00255 14400.00 10000.00 0.00 -0.00187 14600.00 10000.00 0.00 -0.00098 14800.00 10000.00 0.00 -0.00006 15000.00 10000.00 0.00 0.00069 15200.00 10000.00 0.00 0.00140 15400.00 10000.00 0.00 0.00223 15600.00 10000.00 0.00 0.00309 15800.00 10000.00 0.00 0.00398 16000.00 10000.00 0.00 0.00491 16200.00 10000.00 0.00 0.00598 16400.00 10000.00 0.00 0.00711 16600.00 10000.00 0.00 0.00825 16800.00 10000.00 0.00 0.00932 17000.00 10000.00 0.00 0.01049 17200.00 10000.00 0.00 0.01192 17400.00 10000.00 0.00 0.01339 17600.00 10000.00 0.00 0.01492 17800.00 10000.00 0.00 0.01648 18000.00 10000.00 0.00 0.01777 18200.00 10000.00 0.00 0.01892 18400.00 10000.00 0.00 0.02021 18600.00 10000.00 0.00 0.02169 18800.00 10000.00 0.00 0.02313 19000.00 10000.00 0.00 0.02458 19200.00 10000.00 0.00 0.02621 19400.00 10000.00 0.00 0.02778 19600.00 10000.00 0.00 0.02946 19800.00 10000.00 0.00 0.03126 20000.00 10000.00 0.00 0.03308 20200.00 10000.00 0.00 0.03508 20400.00 10000.00 0.00 0.03709 20600.00 10000.00 0.00 0.03908 20800.00 10000.00 0.00 0.04102 21000.00 10000.00 0.00 0.04257 21200.00 10000.00 0.00 0.04349 21400.00 10000.00 0.00 0.04396 21600.00 10000.00 0.00 0.04410 22000.00 10000.00 0.00 0.04411 22200.00 10000.00 0.00 0.04412 21400.00 10200.00 0.00 0.04454 21600.00 10200.00 0.00 0.04469 22000.00 10200.00 0.00 0.04474 22200.00 10200.00 0.00 0.04475 21200.00 10400.00 0.00 0.04476 21400.00 10400.00 0.00 0.04522 21600.00 10400.00 0.00 0.04538 21800.00 10400.00 0.00 0.04539 22000.00 10400.00 0.00 0.04546 22200.00 10400.00 0.00 0.04549 21200.00 10600.00 0.00 0.04556 21400.00 10600.00 0.00 0.04600 21600.00 10600.00 0.00 0.04616 21800.00 10600.00 0.00 0.04620 22000.00 10600.00 0.00 0.04628 22200.00 10600.00 0.00 0.04631 21200.00 10800.00 0.00 0.04636 21400.00 10800.00 0.00 0.04676 21600.00 10800.00 0.00 0.04694 21800.00 10800.00 0.00 0.04700 22000.00 10800.00 0.00 0.04708 22200.00 10800.00 0.00 0.04710 21400.00 11000.00 0.00 0.04742 21600.00 11000.00 0.00 0.04764 21800.00 11000.00 0.00 0.04773 22000.00 11000.00 0.00 0.04782 22200.00 11000.00 0.00 0.04782 21400.00 11200.00 0.00 0.04805 21600.00 11200.00 0.00 0.04832 21800.00 11200.00 0.00 0.04842 22000.00 11200.00 0.00 0.04848 21400.00 11400.00 0.00 0.04886 21600.00 11400.00 0.00 0.04915 21800.00 11400.00 0.00 0.04921 22000.00 11400.00 0.00 0.04926 21400.00 11600.00 0.00 0.04964 21600.00 11600.00 0.00 0.04992 21800.00 11600.00 0.00 0.04996 22000.00 11600.00 0.00 0.05003 22200.00 11600.00 0.00 0.05004 21400.00 11800.00 0.00 0.05040 21600.00 11800.00 0.00 0.05067 21800.00 11800.00 0.00 0.05070 22000.00 11800.00 0.00 0.05078 21400.00 12000.00 0.00 0.05121 21600.00 12000.00 0.00 0.05144 21800.00 12000.00 0.00 0.05147 22000.00 12000.00 0.00 0.05153 21200.00 12200.00 0.00 0.05155 21400.00 12200.00 0.00 0.05207 21600.00 12200.00 0.00 0.05225 21200.00 12400.00 0.00 0.05236 21400.00 12400.00 0.00 0.05281 21600.00 12400.00 0.00 0.05294 21200.00 12600.00 0.00 0.05314 21400.00 12600.00 0.00 0.05357 21600.00 12600.00 0.00 0.05371 21800.00 12600.00 0.00 0.05373 22000.00 12600.00 0.00 0.05374 21200.00 12800.00 0.00 0.05415 21400.00 12800.00 0.00 0.05459 21600.00 12800.00 0.00 0.05476 21800.00 12800.00 0.00 0.05480 22000.00 12800.00 0.00 0.05482 21200.00 13000.00 0.00 0.05506 21400.00 13000.00 0.00 0.05551 21600.00 13000.00 0.00 0.05570 21800.00 13000.00 0.00 0.05572 21200.00 13200.00 0.00 0.05608 21400.00 13200.00 0.00 0.05654 21600.00 13200.00 0.00 0.05672 21200.00 13400.00 0.00 0.05726 21400.00 13400.00 0.00 0.05773 21600.00 13400.00 0.00 0.05792 21200.00 13600.00 0.00 0.05851 21400.00 13600.00 0.00 0.05901 21600.00 13600.00 0.00 0.05919 21200.00 13800.00 0.00 0.05979 21400.00 13800.00 0.00 0.06028 21600.00 13800.00 0.00 0.06041 21200.00 14000.00 0.00 0.06104 21400.00 14000.00 0.00 0.06149 21600.00 14000.00 0.00 0.06156 21200.00 14200.00 0.00 0.06239 21400.00 14200.00 0.00 0.06278 21600.00 14200.00 0.00 0.06283 21200.00 14400.00 0.00 0.06365 21400.00 14400.00 0.00 0.06398 21600.00 14400.00 0.00 0.06403 21000.00 14600.00 0.00 0.06414 21200.00 14600.00 0.00 0.06494 21400.00 14600.00 0.00 0.06529 21600.00 14600.00 0.00 0.06539 21800.00 14600.00 0.00 0.06541 21000.00 14800.00 0.00 0.06559 21200.00 14800.00 0.00 0.06640 21400.00 14800.00 0.00 0.06678 21600.00 14800.00 0.00 0.06692 21000.00 15000.00 0.00 0.06719 21200.00 15000.00 0.00 0.06801 21400.00 15000.00 0.00 0.06841 21600.00 15000.00 0.00 0.06852 21000.00 15200.00 0.00 0.06877 21200.00 15200.00 0.00 0.06963 21400.00 15200.00 0.00 0.07003 21600.00 15200.00 0.00 0.07012 21000.00 15400.00 0.00 0.07033 21200.00 15400.00 0.00 0.07122 21400.00 15400.00 0.00 0.07161 21600.00 15400.00 0.00 0.07163 21000.00 15600.00 0.00 0.07192 21200.00 15600.00 0.00 0.07283 21400.00 15600.00 0.00 0.07315 21000.00 15800.00 0.00 0.07356 21200.00 15800.00 0.00 0.07443 21400.00 15800.00 0.00 0.07466 21000.00 16000.00 0.00 0.07529 21200.00 16000.00 0.00 0.07610 21400.00 16000.00 0.00 0.07628 21000.00 16200.00 0.00 0.07689 21200.00 16200.00 0.00 0.07769 21400.00 16200.00 0.00 0.07789 21000.00 16400.00 0.00 0.07872 21200.00 16400.00 0.00 0.07952 21400.00 16400.00 0.00 0.07971 21000.00 16600.00 0.00 0.08045 21200.00 16600.00 0.00 0.08121 21400.00 16600.00 0.00 0.08134 21000.00 16800.00 0.00 0.08200 21200.00 16800.00 0.00 0.08270 21400.00 16800.00 0.00 0.08275 21000.00 17000.00 0.00 0.08363 21200.00 17000.00 0.00 0.08427 21400.00 17000.00 0.00 0.08428 21000.00 17200.00 0.00 0.08523 21200.00 17200.00 0.00 0.08586 21400.00 17200.00 0.00 0.08586 21000.00 17400.00 0.00 0.08707 21200.00 17400.00 0.00 0.08770 21000.00 17600.00 0.00 0.08890 21200.00 17600.00 0.00 0.08950 21000.00 17800.00 0.00 0.09078 21200.00 17800.00 0.00 0.09135 21000.00 18000.00 0.00 0.09269 21200.00 18000.00 0.00 0.09324 21000.00 18200.00 0.00 0.09462 21200.00 18200.00 0.00 0.09512 21000.00 18400.00 0.00 0.09653 21200.00 18400.00 0.00 0.09696 21000.00 18600.00 0.00 0.09839 21200.00 18600.00 0.00 0.09875 20800.00 18800.00 0.00 0.09903 21000.00 18800.00 0.00 0.10050 21200.00 18800.00 0.00 0.10081 20800.00 19000.00 0.00 0.10133 21000.00 19000.00 0.00 0.10279 21200.00 19000.00 0.00 0.10305 20800.00 19200.00 0.00 0.10397 21000.00 19200.00 0.00 0.10540 21200.00 19200.00 0.00 0.10554 20800.00 19400.00 0.00 0.10687 21000.00 19400.00 0.00 0.10821 20800.00 19600.00 0.00 0.10991 21000.00 19600.00 0.00 0.11115 20800.00 19800.00 0.00 0.11294 21000.00 19800.00 0.00 0.11411 20800.00 20000.00 0.00 0.11629 21000.00 20000.00 0.00 0.11731 20800.00 20200.00 0.00 0.11963 21000.00 20200.00 0.00 0.12035 20800.00 20400.00 0.00 0.12256 21000.00 20400.00 0.00 0.12292 20600.00 20600.00 0.00 0.12348 20800.00 20600.00 0.00 0.12575 20600.00 20800.00 0.00 0.12651 20800.00 20800.00 0.00 0.12795 20800.00 21000.00 0.00 0.12825 20800.00 21000.00 5.00 0.12840 20800.00 21000.00 10.00 0.12852 20800.00 21000.00 15.00 0.12862 20800.00 21000.00 20.00 0.12868 20800.00 21000.00 25.00 0.12870 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20745.37 20991.10 25.00 0.12883 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/493.ctf NX, NY, NZ: 512 512 1 MODE : real