CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/489.mrc Output diagnostic file name Micrographs_0601/489.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/489.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2610.000 8614.000 Mean, RMS : 4505.532 213.8369 TITLE 1: emi2mrc: 489.emi Mon Jan 06 16:20:57 2014 TITLE 2: kV 300, Mag 78000x, Def 17560, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 23.707, Y 484.542, Z -31.476, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.07335 10200.00 10000.00 0.00 0.07813 10400.00 10000.00 0.00 0.08265 10600.00 10000.00 0.00 0.08787 10800.00 10000.00 0.00 0.09297 11000.00 10000.00 0.00 0.09820 11200.00 10000.00 0.00 0.10372 11400.00 10000.00 0.00 0.10934 11600.00 10000.00 0.00 0.11541 11800.00 10000.00 0.00 0.12025 12000.00 10000.00 0.00 0.12188 11800.00 10200.00 0.00 0.12474 12000.00 10200.00 0.00 0.12613 11800.00 10400.00 0.00 0.12926 12000.00 10400.00 0.00 0.13039 11800.00 10600.00 0.00 0.13386 12000.00 10600.00 0.00 0.13466 11800.00 10800.00 0.00 0.13798 12000.00 10800.00 0.00 0.13835 11800.00 11000.00 0.00 0.14227 11600.00 11200.00 0.00 0.14232 11800.00 11200.00 0.00 0.14623 11600.00 11400.00 0.00 0.14702 11800.00 11400.00 0.00 0.15048 11600.00 11600.00 0.00 0.15120 11800.00 11600.00 0.00 0.15405 11600.00 11800.00 0.00 0.15476 11800.00 11800.00 0.00 0.15711 11800.00 12000.00 0.00 0.15751 11800.00 12000.00 5.00 0.15763 11800.00 12000.00 10.00 0.15769 11800.00 12000.00 15.00 0.15769 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11753.27 11947.08 12.38 0.15817 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/489.ctf NX, NY, NZ: 512 512 1 MODE : real