CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/488.mrc Output diagnostic file name Micrographs_0601/488.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/488.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2330.000 7946.000 Mean, RMS : 4497.653 215.5306 TITLE 1: emi2mrc: 488.emi Mon Jan 06 16:19:47 2014 TITLE 2: kV 300, Mag 78000x, Def 17560, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 21.974, Y 486.710, Z -31.476, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02344 10200.00 10000.00 0.00 0.02510 10400.00 10000.00 0.00 0.02663 10600.00 10000.00 0.00 0.02839 10800.00 10000.00 0.00 0.03023 11000.00 10000.00 0.00 0.03225 11200.00 10000.00 0.00 0.03441 11400.00 10000.00 0.00 0.03679 11600.00 10000.00 0.00 0.03917 11800.00 10000.00 0.00 0.04164 12000.00 10000.00 0.00 0.04450 12200.00 10000.00 0.00 0.04724 12400.00 10000.00 0.00 0.05023 12600.00 10000.00 0.00 0.05347 12800.00 10000.00 0.00 0.05663 13000.00 10000.00 0.00 0.05965 13200.00 10000.00 0.00 0.06276 13400.00 10000.00 0.00 0.06614 13600.00 10000.00 0.00 0.06987 13800.00 10000.00 0.00 0.07395 14000.00 10000.00 0.00 0.07785 14200.00 10000.00 0.00 0.08150 14400.00 10000.00 0.00 0.08381 14600.00 10000.00 0.00 0.08453 14800.00 10000.00 0.00 0.08460 14400.00 10200.00 0.00 0.08596 14600.00 10200.00 0.00 0.08668 14800.00 10200.00 0.00 0.08677 14400.00 10400.00 0.00 0.08823 14600.00 10400.00 0.00 0.08895 14800.00 10400.00 0.00 0.08903 14400.00 10600.00 0.00 0.09055 14600.00 10600.00 0.00 0.09128 14800.00 10600.00 0.00 0.09133 14400.00 10800.00 0.00 0.09271 14600.00 10800.00 0.00 0.09347 14800.00 10800.00 0.00 0.09349 14400.00 11000.00 0.00 0.09521 14600.00 11000.00 0.00 0.09597 14400.00 11200.00 0.00 0.09795 14600.00 11200.00 0.00 0.09865 14400.00 11400.00 0.00 0.10100 14600.00 11400.00 0.00 0.10162 14200.00 11600.00 0.00 0.10170 14400.00 11600.00 0.00 0.10419 14600.00 11600.00 0.00 0.10474 14200.00 11800.00 0.00 0.10522 14400.00 11800.00 0.00 0.10768 14600.00 11800.00 0.00 0.10815 14200.00 12000.00 0.00 0.10875 14400.00 12000.00 0.00 0.11117 14600.00 12000.00 0.00 0.11146 14200.00 12200.00 0.00 0.11223 14400.00 12200.00 0.00 0.11452 14600.00 12200.00 0.00 0.11461 14200.00 12400.00 0.00 0.11592 14400.00 12400.00 0.00 0.11810 14200.00 12600.00 0.00 0.11946 14400.00 12600.00 0.00 0.12158 14200.00 12800.00 0.00 0.12329 14400.00 12800.00 0.00 0.12532 14200.00 13000.00 0.00 0.12703 14400.00 13000.00 0.00 0.12892 14200.00 13200.00 0.00 0.13117 14400.00 13200.00 0.00 0.13293 14200.00 13400.00 0.00 0.13540 14400.00 13400.00 0.00 0.13690 14200.00 13600.00 0.00 0.13950 14400.00 13600.00 0.00 0.14060 14200.00 13800.00 0.00 0.14352 14400.00 13800.00 0.00 0.14413 14200.00 14000.00 0.00 0.14702 14400.00 14000.00 0.00 0.14702 14200.00 14200.00 0.00 0.15052 14200.00 14400.00 0.00 0.15135 14200.00 14400.00 5.00 0.15138 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14219.79 14328.48 9.39 0.15161 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/488.ctf NX, NY, NZ: 512 512 1 MODE : real