CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/486.mrc Output diagnostic file name Micrographs_0601/486.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/486.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2258.000 9150.000 Mean, RMS : 4477.077 211.5266 TITLE 1: emi2mrc: 486.emi Mon Jan 06 16:08:56 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.05%, Obj 88.09%, Dif 73.67% TITLE 3: X 18.940, Y 491.170, Z -31.476, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.09105 10200.00 10000.00 0.00 0.09649 10400.00 10000.00 0.00 0.10157 10600.00 10000.00 0.00 0.10762 10800.00 10000.00 0.00 0.11367 11000.00 10000.00 0.00 0.12032 11200.00 10000.00 0.00 0.12633 11400.00 10000.00 0.00 0.12933 11200.00 10200.00 0.00 0.13147 11400.00 10200.00 0.00 0.13424 11200.00 10400.00 0.00 0.13663 11400.00 10400.00 0.00 0.13910 11200.00 10600.00 0.00 0.14199 11400.00 10600.00 0.00 0.14401 11200.00 10800.00 0.00 0.14694 11400.00 10800.00 0.00 0.14832 11200.00 11000.00 0.00 0.15196 11400.00 11000.00 0.00 0.15244 11200.00 11200.00 0.00 0.15523 11200.00 11400.00 0.00 0.15748 11200.00 11400.00 5.00 0.15754 11200.00 11400.00 10.00 0.15756 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11232.69 11476.36 10.00 0.15793 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/486.ctf NX, NY, NZ: 512 512 1 MODE : real