CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/484.mrc Output diagnostic file name Micrographs_0601/484.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/484.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2156.000 8780.000 Mean, RMS : 4454.012 214.7873 TITLE 1: emi2mrc: 484.emi Mon Jan 06 16:06:11 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.05%, Obj 88.11%, Dif 73.67% TITLE 3: X 14.180, Y 497.320, Z -31.476, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04173 10200.00 10000.00 0.00 0.04447 10400.00 10000.00 0.00 0.04714 10600.00 10000.00 0.00 0.05028 10800.00 10000.00 0.00 0.05346 11000.00 10000.00 0.00 0.05666 11200.00 10000.00 0.00 0.05994 11400.00 10000.00 0.00 0.06364 11600.00 10000.00 0.00 0.06771 11800.00 10000.00 0.00 0.07182 12000.00 10000.00 0.00 0.07612 12200.00 10000.00 0.00 0.08046 12400.00 10000.00 0.00 0.08512 12600.00 10000.00 0.00 0.09003 12800.00 10000.00 0.00 0.09521 13000.00 10000.00 0.00 0.09922 13200.00 10000.00 0.00 0.10110 13400.00 10000.00 0.00 0.10144 13000.00 10200.00 0.00 0.10256 13200.00 10200.00 0.00 0.10435 13400.00 10200.00 0.00 0.10452 13000.00 10400.00 0.00 0.10600 13200.00 10400.00 0.00 0.10768 13400.00 10400.00 0.00 0.10773 13000.00 10600.00 0.00 0.10969 13200.00 10600.00 0.00 0.11130 13000.00 10800.00 0.00 0.11356 13200.00 10800.00 0.00 0.11502 13000.00 11000.00 0.00 0.11780 13200.00 11000.00 0.00 0.11905 13000.00 11200.00 0.00 0.12194 13200.00 11200.00 0.00 0.12300 13000.00 11400.00 0.00 0.12640 13200.00 11400.00 0.00 0.12725 13000.00 11600.00 0.00 0.13083 13200.00 11600.00 0.00 0.13145 12800.00 11800.00 0.00 0.13186 13000.00 11800.00 0.00 0.13556 13200.00 11800.00 0.00 0.13593 12800.00 12000.00 0.00 0.13662 13000.00 12000.00 0.00 0.14024 13200.00 12000.00 0.00 0.14027 12800.00 12200.00 0.00 0.14154 13000.00 12200.00 0.00 0.14498 12800.00 12400.00 0.00 0.14685 13000.00 12400.00 0.00 0.14994 12800.00 12600.00 0.00 0.15141 13000.00 12600.00 0.00 0.15411 12800.00 12800.00 0.00 0.15682 13000.00 12800.00 0.00 0.15854 12800.00 13000.00 0.00 0.16059 12800.00 13000.00 5.00 0.16069 12800.00 13000.00 10.00 0.16076 12800.00 13000.00 15.00 0.16079 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12883.60 13033.53 9.88 0.16150 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/484.ctf NX, NY, NZ: 512 512 1 MODE : real