CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/477.mrc Output diagnostic file name Micrographs_0601/477.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/477.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2048.000 8172.000 Mean, RMS : 4501.554 218.6247 TITLE 1: emi2mrc: 477.emi Mon Jan 06 15:58:06 2014 TITLE 2: kV 300, Mag 78000x, Def 18870, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 17.971, Y 488.132, Z -32.203, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02015 10400.00 10000.00 0.00 -0.01999 10600.00 10000.00 0.00 -0.01981 10800.00 10000.00 0.00 -0.01948 11000.00 10000.00 0.00 -0.01924 11200.00 10000.00 0.00 -0.01909 11400.00 10000.00 0.00 -0.01881 11600.00 10000.00 0.00 -0.01855 11800.00 10000.00 0.00 -0.01830 12000.00 10000.00 0.00 -0.01808 12200.00 10000.00 0.00 -0.01797 12400.00 10000.00 0.00 -0.01783 12600.00 10000.00 0.00 -0.01773 12800.00 10000.00 0.00 -0.01770 13000.00 10000.00 0.00 -0.01755 13600.00 10000.00 0.00 -0.01740 13800.00 10000.00 0.00 -0.01721 14000.00 10000.00 0.00 -0.01690 14200.00 10000.00 0.00 -0.01656 14400.00 10000.00 0.00 -0.01614 14600.00 10000.00 0.00 -0.01569 14800.00 10000.00 0.00 -0.01535 15000.00 10000.00 0.00 -0.01502 15200.00 10000.00 0.00 -0.01448 15400.00 10000.00 0.00 -0.01371 15600.00 10000.00 0.00 -0.01290 15800.00 10000.00 0.00 -0.01214 16000.00 10000.00 0.00 -0.01161 16200.00 10000.00 0.00 -0.01092 16400.00 10000.00 0.00 -0.00988 16600.00 10000.00 0.00 -0.00879 16800.00 10000.00 0.00 -0.00771 17000.00 10000.00 0.00 -0.00671 17200.00 10000.00 0.00 -0.00579 17400.00 10000.00 0.00 -0.00491 17600.00 10000.00 0.00 -0.00391 17800.00 10000.00 0.00 -0.00300 18000.00 10000.00 0.00 -0.00223 18200.00 10000.00 0.00 -0.00120 18400.00 10000.00 0.00 -0.00009 18600.00 10000.00 0.00 0.00092 18800.00 10000.00 0.00 0.00197 19000.00 10000.00 0.00 0.00310 19200.00 10000.00 0.00 0.00430 19400.00 10000.00 0.00 0.00534 19600.00 10000.00 0.00 0.00634 19800.00 10000.00 0.00 0.00745 20000.00 10000.00 0.00 0.00854 20200.00 10000.00 0.00 0.00952 20400.00 10000.00 0.00 0.01056 20600.00 10000.00 0.00 0.01174 20800.00 10000.00 0.00 0.01292 21000.00 10000.00 0.00 0.01405 21200.00 10000.00 0.00 0.01531 21400.00 10000.00 0.00 0.01680 21600.00 10000.00 0.00 0.01828 21800.00 10000.00 0.00 0.01970 22000.00 10000.00 0.00 0.02130 22200.00 10000.00 0.00 0.02297 22400.00 10000.00 0.00 0.02483 22600.00 10000.00 0.00 0.02680 22800.00 10000.00 0.00 0.02852 23000.00 10000.00 0.00 0.03000 23200.00 10000.00 0.00 0.03121 23400.00 10000.00 0.00 0.03219 23600.00 10000.00 0.00 0.03298 23800.00 10000.00 0.00 0.03336 24000.00 10000.00 0.00 0.03339 24400.00 10000.00 0.00 0.03344 24600.00 10000.00 0.00 0.03346 23600.00 10200.00 0.00 0.03348 23800.00 10200.00 0.00 0.03383 24000.00 10200.00 0.00 0.03391 24200.00 10200.00 0.00 0.03394 24400.00 10200.00 0.00 0.03397 23600.00 10400.00 0.00 0.03404 23800.00 10400.00 0.00 0.03441 24000.00 10400.00 0.00 0.03452 24200.00 10400.00 0.00 0.03452 23600.00 10600.00 0.00 0.03456 23800.00 10600.00 0.00 0.03493 24000.00 10600.00 0.00 0.03500 23600.00 10800.00 0.00 0.03517 23800.00 10800.00 0.00 0.03550 24000.00 10800.00 0.00 0.03554 23600.00 11000.00 0.00 0.03584 23800.00 11000.00 0.00 0.03614 23600.00 11200.00 0.00 0.03629 23800.00 11200.00 0.00 0.03656 23600.00 11400.00 0.00 0.03683 23800.00 11400.00 0.00 0.03708 23600.00 11600.00 0.00 0.03743 23800.00 11600.00 0.00 0.03769 24000.00 11600.00 0.00 0.03770 23600.00 11800.00 0.00 0.03800 23800.00 11800.00 0.00 0.03825 23600.00 12000.00 0.00 0.03845 23800.00 12000.00 0.00 0.03867 23600.00 12200.00 0.00 0.03891 23800.00 12200.00 0.00 0.03912 23600.00 12400.00 0.00 0.03961 23800.00 12400.00 0.00 0.03982 23400.00 12600.00 0.00 0.03987 23600.00 12600.00 0.00 0.04037 23800.00 12600.00 0.00 0.04054 23400.00 12800.00 0.00 0.04064 23600.00 12800.00 0.00 0.04104 23800.00 12800.00 0.00 0.04116 23400.00 13000.00 0.00 0.04120 23600.00 13000.00 0.00 0.04159 23800.00 13000.00 0.00 0.04177 23400.00 13200.00 0.00 0.04185 23600.00 13200.00 0.00 0.04232 23800.00 13200.00 0.00 0.04258 23600.00 13400.00 0.00 0.04306 23800.00 13400.00 0.00 0.04331 23600.00 13600.00 0.00 0.04371 23800.00 13600.00 0.00 0.04393 23400.00 13800.00 0.00 0.04404 23600.00 13800.00 0.00 0.04458 23800.00 13800.00 0.00 0.04483 23400.00 14000.00 0.00 0.04500 23600.00 14000.00 0.00 0.04557 23800.00 14000.00 0.00 0.04583 23400.00 14200.00 0.00 0.04602 23600.00 14200.00 0.00 0.04657 23800.00 14200.00 0.00 0.04677 23400.00 14400.00 0.00 0.04700 23600.00 14400.00 0.00 0.04749 23800.00 14400.00 0.00 0.04765 23400.00 14600.00 0.00 0.04794 23600.00 14600.00 0.00 0.04842 23800.00 14600.00 0.00 0.04859 23400.00 14800.00 0.00 0.04886 23600.00 14800.00 0.00 0.04937 23800.00 14800.00 0.00 0.04953 23400.00 15000.00 0.00 0.04971 23600.00 15000.00 0.00 0.05024 23800.00 15000.00 0.00 0.05034 23400.00 15200.00 0.00 0.05067 23600.00 15200.00 0.00 0.05118 23800.00 15200.00 0.00 0.05120 23400.00 15400.00 0.00 0.05180 23600.00 15400.00 0.00 0.05226 23400.00 15600.00 0.00 0.05302 23600.00 15600.00 0.00 0.05348 23800.00 15600.00 0.00 0.05350 23200.00 15800.00 0.00 0.05354 23400.00 15800.00 0.00 0.05433 23600.00 15800.00 0.00 0.05478 23800.00 15800.00 0.00 0.05479 23400.00 16000.00 0.00 0.05555 23600.00 16000.00 0.00 0.05598 23400.00 16200.00 0.00 0.05662 23600.00 16200.00 0.00 0.05703 23400.00 16400.00 0.00 0.05773 23600.00 16400.00 0.00 0.05810 23400.00 16600.00 0.00 0.05886 23600.00 16600.00 0.00 0.05918 23200.00 16800.00 0.00 0.05931 23400.00 16800.00 0.00 0.06007 23600.00 16800.00 0.00 0.06037 23200.00 17000.00 0.00 0.06056 23400.00 17000.00 0.00 0.06133 23600.00 17000.00 0.00 0.06163 23200.00 17200.00 0.00 0.06198 23400.00 17200.00 0.00 0.06272 23600.00 17200.00 0.00 0.06297 23200.00 17400.00 0.00 0.06339 23400.00 17400.00 0.00 0.06409 23600.00 17400.00 0.00 0.06430 23200.00 17600.00 0.00 0.06464 23400.00 17600.00 0.00 0.06535 23600.00 17600.00 0.00 0.06555 23200.00 17800.00 0.00 0.06612 23400.00 17800.00 0.00 0.06684 23600.00 17800.00 0.00 0.06700 23200.00 18000.00 0.00 0.06753 23400.00 18000.00 0.00 0.06817 23600.00 18000.00 0.00 0.06822 23200.00 18200.00 0.00 0.06881 23400.00 18200.00 0.00 0.06937 23200.00 18400.00 0.00 0.07011 23400.00 18400.00 0.00 0.07065 23200.00 18600.00 0.00 0.07155 23400.00 18600.00 0.00 0.07206 23200.00 18800.00 0.00 0.07302 23400.00 18800.00 0.00 0.07347 23200.00 19000.00 0.00 0.07434 23400.00 19000.00 0.00 0.07474 23200.00 19200.00 0.00 0.07578 23400.00 19200.00 0.00 0.07615 23200.00 19400.00 0.00 0.07723 23400.00 19400.00 0.00 0.07760 23200.00 19600.00 0.00 0.07867 23400.00 19600.00 0.00 0.07904 23200.00 19800.00 0.00 0.08000 23400.00 19800.00 0.00 0.08038 23000.00 20000.00 0.00 0.08045 23200.00 20000.00 0.00 0.08161 23400.00 20000.00 0.00 0.08198 23000.00 20200.00 0.00 0.08229 23200.00 20200.00 0.00 0.08347 23400.00 20200.00 0.00 0.08380 23000.00 20400.00 0.00 0.08407 23200.00 20400.00 0.00 0.08522 23400.00 20400.00 0.00 0.08544 23000.00 20600.00 0.00 0.08590 23200.00 20600.00 0.00 0.08695 23400.00 20600.00 0.00 0.08704 23000.00 20800.00 0.00 0.08754 23200.00 20800.00 0.00 0.08848 23400.00 20800.00 0.00 0.08848 23000.00 21000.00 0.00 0.08924 23200.00 21000.00 0.00 0.09010 23000.00 21200.00 0.00 0.09080 23200.00 21200.00 0.00 0.09160 23000.00 21400.00 0.00 0.09234 23200.00 21400.00 0.00 0.09308 23000.00 21600.00 0.00 0.09388 23200.00 21600.00 0.00 0.09453 23000.00 21800.00 0.00 0.09538 23200.00 21800.00 0.00 0.09593 23000.00 22000.00 0.00 0.09713 23200.00 22000.00 0.00 0.09758 23000.00 22200.00 0.00 0.09890 23200.00 22200.00 0.00 0.09924 23000.00 22400.00 0.00 0.10094 23200.00 22400.00 0.00 0.10111 23000.00 22600.00 0.00 0.10271 22800.00 22800.00 0.00 0.10286 23000.00 22800.00 0.00 0.10445 22800.00 23000.00 0.00 0.10457 23000.00 23000.00 0.00 0.10569 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22979.11 23040.64 13.35 0.10574 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/477.ctf NX, NY, NZ: 512 512 1 MODE : real