CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/474.mrc Output diagnostic file name Micrographs_0601/474.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/474.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2390.000 7712.000 Mean, RMS : 4504.555 217.0235 TITLE 1: emi2mrc: 474.emi Mon Jan 06 15:55:15 2014 TITLE 2: kV 300, Mag 78000x, Def 18870, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 24.592, Y 479.196, Z -32.203, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03216 10200.00 10000.00 0.00 0.03426 10400.00 10000.00 0.00 0.03635 10600.00 10000.00 0.00 0.03867 10800.00 10000.00 0.00 0.04090 11000.00 10000.00 0.00 0.04338 11200.00 10000.00 0.00 0.04611 11400.00 10000.00 0.00 0.04888 11600.00 10000.00 0.00 0.05173 11800.00 10000.00 0.00 0.05477 12000.00 10000.00 0.00 0.05802 12200.00 10000.00 0.00 0.06117 12400.00 10000.00 0.00 0.06462 12600.00 10000.00 0.00 0.06810 12800.00 10000.00 0.00 0.07171 13000.00 10000.00 0.00 0.07572 13200.00 10000.00 0.00 0.08000 13400.00 10000.00 0.00 0.08477 13600.00 10000.00 0.00 0.08926 13800.00 10000.00 0.00 0.09197 14000.00 10000.00 0.00 0.09246 13800.00 10200.00 0.00 0.09449 14000.00 10200.00 0.00 0.09496 13800.00 10400.00 0.00 0.09721 14000.00 10400.00 0.00 0.09762 13800.00 10600.00 0.00 0.09996 14000.00 10600.00 0.00 0.10031 13800.00 10800.00 0.00 0.10285 14000.00 10800.00 0.00 0.10315 13600.00 11000.00 0.00 0.10342 13800.00 11000.00 0.00 0.10604 14000.00 11000.00 0.00 0.10625 13600.00 11200.00 0.00 0.10657 13800.00 11200.00 0.00 0.10919 14000.00 11200.00 0.00 0.10928 13600.00 11400.00 0.00 0.11015 13800.00 11400.00 0.00 0.11274 13600.00 11600.00 0.00 0.11376 13800.00 11600.00 0.00 0.11631 13600.00 11800.00 0.00 0.11766 13800.00 11800.00 0.00 0.12018 13600.00 12000.00 0.00 0.12181 13800.00 12000.00 0.00 0.12426 13600.00 12200.00 0.00 0.12601 13800.00 12200.00 0.00 0.12838 13600.00 12400.00 0.00 0.13038 13800.00 12400.00 0.00 0.13269 13600.00 12600.00 0.00 0.13470 13800.00 12600.00 0.00 0.13692 13600.00 12800.00 0.00 0.13956 13800.00 12800.00 0.00 0.14159 13600.00 13000.00 0.00 0.14430 13800.00 13000.00 0.00 0.14605 13600.00 13200.00 0.00 0.14936 13800.00 13200.00 0.00 0.15059 13600.00 13400.00 0.00 0.15377 13800.00 13400.00 0.00 0.15428 13600.00 13600.00 0.00 0.15789 13400.00 13800.00 0.00 0.15793 13600.00 13800.00 0.00 0.16075 13600.00 13800.00 5.00 0.16079 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13619.19 13808.35 5.82 0.16084 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/474.ctf NX, NY, NZ: 512 512 1 MODE : real