CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/473.mrc Output diagnostic file name Micrographs_0601/473.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/473.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2328.000 8010.000 Mean, RMS : 4518.287 217.8222 TITLE 1: emi2mrc: 473.emi Mon Jan 06 15:53:52 2014 TITLE 2: kV 300, Mag 78000x, Def 19690, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 20.963, Y 479.587, Z -32.203, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01888 10200.00 10000.00 0.00 0.01988 10400.00 10000.00 0.00 0.02074 10600.00 10000.00 0.00 0.02207 10800.00 10000.00 0.00 0.02349 11000.00 10000.00 0.00 0.02497 11200.00 10000.00 0.00 0.02662 11400.00 10000.00 0.00 0.02839 11600.00 10000.00 0.00 0.03026 11800.00 10000.00 0.00 0.03236 12000.00 10000.00 0.00 0.03461 12200.00 10000.00 0.00 0.03691 12400.00 10000.00 0.00 0.03959 12600.00 10000.00 0.00 0.04223 12800.00 10000.00 0.00 0.04488 13000.00 10000.00 0.00 0.04791 13200.00 10000.00 0.00 0.05113 13400.00 10000.00 0.00 0.05430 13600.00 10000.00 0.00 0.05745 13800.00 10000.00 0.00 0.06092 14000.00 10000.00 0.00 0.06461 14200.00 10000.00 0.00 0.06847 14400.00 10000.00 0.00 0.07238 14600.00 10000.00 0.00 0.07663 14800.00 10000.00 0.00 0.08017 15000.00 10000.00 0.00 0.08154 15200.00 10000.00 0.00 0.08170 14800.00 10200.00 0.00 0.08179 15000.00 10200.00 0.00 0.08318 15200.00 10200.00 0.00 0.08337 14800.00 10400.00 0.00 0.08367 15000.00 10400.00 0.00 0.08505 15200.00 10400.00 0.00 0.08523 14800.00 10600.00 0.00 0.08572 15000.00 10600.00 0.00 0.08709 15200.00 10600.00 0.00 0.08721 14800.00 10800.00 0.00 0.08783 15000.00 10800.00 0.00 0.08923 15200.00 10800.00 0.00 0.08931 14800.00 11000.00 0.00 0.09052 15000.00 11000.00 0.00 0.09194 14800.00 11200.00 0.00 0.09324 15000.00 11200.00 0.00 0.09458 14800.00 11400.00 0.00 0.09597 15000.00 11400.00 0.00 0.09724 14800.00 11600.00 0.00 0.09884 15000.00 11600.00 0.00 0.10007 14800.00 11800.00 0.00 0.10185 15000.00 11800.00 0.00 0.10301 14800.00 12000.00 0.00 0.10499 15000.00 12000.00 0.00 0.10603 14800.00 12200.00 0.00 0.10826 15000.00 12200.00 0.00 0.10919 14800.00 12400.00 0.00 0.11163 15000.00 12400.00 0.00 0.11241 14800.00 12600.00 0.00 0.11511 15000.00 12600.00 0.00 0.11572 14800.00 12800.00 0.00 0.11905 15000.00 12800.00 0.00 0.11948 14800.00 13000.00 0.00 0.12291 15000.00 13000.00 0.00 0.12319 14600.00 13200.00 0.00 0.12332 14800.00 13200.00 0.00 0.12715 15000.00 13200.00 0.00 0.12722 14600.00 13400.00 0.00 0.12759 14800.00 13400.00 0.00 0.13134 14600.00 13600.00 0.00 0.13186 14800.00 13600.00 0.00 0.13553 14600.00 13800.00 0.00 0.13643 14800.00 13800.00 0.00 0.14000 14600.00 14000.00 0.00 0.14124 14800.00 14000.00 0.00 0.14463 14600.00 14200.00 0.00 0.14668 14800.00 14200.00 0.00 0.14974 14600.00 14400.00 0.00 0.15155 14800.00 14400.00 0.00 0.15425 14600.00 14600.00 0.00 0.15743 14800.00 14600.00 0.00 0.15922 14600.00 14800.00 0.00 0.16213 14600.00 14800.00 5.00 0.16221 14600.00 14800.00 10.00 0.16223 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14681.59 14837.84 10.00 0.16318 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/473.ctf NX, NY, NZ: 512 512 1 MODE : real