CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/472.mrc Output diagnostic file name Micrographs_0601/472.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/472.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2282.000 8550.000 Mean, RMS : 4468.079 214.8495 TITLE 1: emi2mrc: 472.emi Mon Jan 06 15:52:36 2014 TITLE 2: kV 300, Mag 78000x, Def 19530, Spot 4, Int 55.05%, Obj 88.08%, Dif 73.67% TITLE 3: X 19.427, Y 481.539, Z -32.203, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03399 10200.00 10000.00 0.00 0.03590 10400.00 10000.00 0.00 0.03781 10600.00 10000.00 0.00 0.03994 10800.00 10000.00 0.00 0.04224 11000.00 10000.00 0.00 0.04502 11200.00 10000.00 0.00 0.04763 11400.00 10000.00 0.00 0.05035 11600.00 10000.00 0.00 0.05386 11800.00 10000.00 0.00 0.05750 12000.00 10000.00 0.00 0.06122 12200.00 10000.00 0.00 0.06471 12400.00 10000.00 0.00 0.06823 12600.00 10000.00 0.00 0.07200 12800.00 10000.00 0.00 0.07598 13000.00 10000.00 0.00 0.08011 13200.00 10000.00 0.00 0.08421 13400.00 10000.00 0.00 0.08802 13600.00 10000.00 0.00 0.09066 13800.00 10000.00 0.00 0.09182 13600.00 10200.00 0.00 0.09313 13800.00 10200.00 0.00 0.09428 13600.00 10400.00 0.00 0.09564 13800.00 10400.00 0.00 0.09672 13600.00 10600.00 0.00 0.09825 13800.00 10600.00 0.00 0.09924 13600.00 10800.00 0.00 0.10095 13800.00 10800.00 0.00 0.10184 13600.00 11000.00 0.00 0.10401 13800.00 11000.00 0.00 0.10479 13600.00 11200.00 0.00 0.10701 13800.00 11200.00 0.00 0.10771 13400.00 11400.00 0.00 0.10796 13600.00 11400.00 0.00 0.11038 13800.00 11400.00 0.00 0.11100 13400.00 11600.00 0.00 0.11141 13600.00 11600.00 0.00 0.11376 13800.00 11600.00 0.00 0.11425 13400.00 11800.00 0.00 0.11512 13600.00 11800.00 0.00 0.11735 13800.00 11800.00 0.00 0.11767 13400.00 12000.00 0.00 0.11893 13600.00 12000.00 0.00 0.12104 13800.00 12000.00 0.00 0.12118 13400.00 12200.00 0.00 0.12286 13600.00 12200.00 0.00 0.12484 13400.00 12400.00 0.00 0.12695 13600.00 12400.00 0.00 0.12879 13400.00 12600.00 0.00 0.13064 13600.00 12600.00 0.00 0.13234 13400.00 12800.00 0.00 0.13472 13600.00 12800.00 0.00 0.13614 13400.00 13000.00 0.00 0.13826 13600.00 13000.00 0.00 0.13933 13400.00 13200.00 0.00 0.14196 13600.00 13200.00 0.00 0.14248 13400.00 13400.00 0.00 0.14495 13400.00 13600.00 0.00 0.14609 13400.00 13800.00 0.00 0.14624 13400.00 13800.00 5.00 0.14631 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13407.15 13723.11 7.64 0.14650 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/472.ctf NX, NY, NZ: 512 512 1 MODE : real