CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/471.mrc Output diagnostic file name Micrographs_0601/471.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/471.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2382.000 7942.000 Mean, RMS : 4488.643 213.0311 TITLE 1: emi2mrc: 471.emi Mon Jan 06 15:51:43 2014 TITLE 2: kV 300, Mag 78000x, Def 19530, Spot 4, Int 55.05%, Obj 88.08%, Dif 73.67% TITLE 3: X 17.904, Y 483.607, Z -32.203, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00647 10200.00 10000.00 0.00 0.00752 10400.00 10000.00 0.00 0.00845 10600.00 10000.00 0.00 0.00944 10800.00 10000.00 0.00 0.01047 11000.00 10000.00 0.00 0.01157 11200.00 10000.00 0.00 0.01278 11400.00 10000.00 0.00 0.01405 11600.00 10000.00 0.00 0.01527 11800.00 10000.00 0.00 0.01665 12000.00 10000.00 0.00 0.01823 12200.00 10000.00 0.00 0.01987 12400.00 10000.00 0.00 0.02165 12600.00 10000.00 0.00 0.02343 12800.00 10000.00 0.00 0.02533 13000.00 10000.00 0.00 0.02724 13200.00 10000.00 0.00 0.02931 13400.00 10000.00 0.00 0.03172 13600.00 10000.00 0.00 0.03402 13800.00 10000.00 0.00 0.03653 14000.00 10000.00 0.00 0.03932 14200.00 10000.00 0.00 0.04204 14400.00 10000.00 0.00 0.04484 14600.00 10000.00 0.00 0.04795 14800.00 10000.00 0.00 0.05112 15000.00 10000.00 0.00 0.05420 15200.00 10000.00 0.00 0.05753 15400.00 10000.00 0.00 0.06128 15600.00 10000.00 0.00 0.06547 15800.00 10000.00 0.00 0.06935 16000.00 10000.00 0.00 0.07221 16200.00 10000.00 0.00 0.07359 16400.00 10000.00 0.00 0.07379 16200.00 10200.00 0.00 0.07497 16400.00 10200.00 0.00 0.07511 16200.00 10400.00 0.00 0.07641 16400.00 10400.00 0.00 0.07652 16000.00 10600.00 0.00 0.07667 16200.00 10600.00 0.00 0.07805 16400.00 10600.00 0.00 0.07810 16000.00 10800.00 0.00 0.07840 16200.00 10800.00 0.00 0.07974 16000.00 11000.00 0.00 0.08026 16200.00 11000.00 0.00 0.08157 16000.00 11200.00 0.00 0.08201 16200.00 11200.00 0.00 0.08328 16000.00 11400.00 0.00 0.08393 16200.00 11400.00 0.00 0.08514 16000.00 11600.00 0.00 0.08580 16200.00 11600.00 0.00 0.08697 16000.00 11800.00 0.00 0.08794 16200.00 11800.00 0.00 0.08905 16000.00 12000.00 0.00 0.09013 16200.00 12000.00 0.00 0.09115 16000.00 12200.00 0.00 0.09226 16200.00 12200.00 0.00 0.09322 16000.00 12400.00 0.00 0.09462 16200.00 12400.00 0.00 0.09551 16000.00 12600.00 0.00 0.09672 16200.00 12600.00 0.00 0.09752 16000.00 12800.00 0.00 0.09885 16200.00 12800.00 0.00 0.09957 16000.00 13000.00 0.00 0.10098 16200.00 13000.00 0.00 0.10162 16000.00 13200.00 0.00 0.10326 16200.00 13200.00 0.00 0.10382 16000.00 13400.00 0.00 0.10540 16200.00 13400.00 0.00 0.10586 16000.00 13600.00 0.00 0.10759 16200.00 13600.00 0.00 0.10792 16000.00 13800.00 0.00 0.10993 16200.00 13800.00 0.00 0.11010 16000.00 14000.00 0.00 0.11195 16200.00 14000.00 0.00 0.11200 16000.00 14200.00 0.00 0.11411 16000.00 14400.00 0.00 0.11613 15800.00 14600.00 0.00 0.11627 16000.00 14600.00 0.00 0.11834 15800.00 14800.00 0.00 0.11856 16000.00 14800.00 0.00 0.12056 15800.00 15000.00 0.00 0.12092 16000.00 15000.00 0.00 0.12280 15800.00 15200.00 0.00 0.12323 16000.00 15200.00 0.00 0.12496 15800.00 15400.00 0.00 0.12542 16000.00 15400.00 0.00 0.12693 15800.00 15600.00 0.00 0.12767 16000.00 15600.00 0.00 0.12883 15800.00 15800.00 0.00 0.12901 16000.00 15800.00 0.00 0.13000 15800.00 16000.00 0.00 0.13054 16000.00 16000.00 0.00 0.13112 15800.00 16200.00 0.00 0.13116 15800.00 16200.00 5.00 0.13125 15800.00 16200.00 10.00 0.13130 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15903.31 16133.31 9.15 0.13170 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/471.ctf NX, NY, NZ: 512 512 1 MODE : real