CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/464.mrc Output diagnostic file name Micrographs_0601/464.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/464.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2258.000 7662.000 Mean, RMS : 4482.392 217.7204 TITLE 1: emi2mrc: 464.emi Mon Jan 06 15:33:49 2014 TITLE 2: kV 300, Mag 78000x, Def 19030, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 10.663, Y 488.568, Z -30.176, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00457 10200.00 10000.00 0.00 0.00514 10400.00 10000.00 0.00 0.00562 10600.00 10000.00 0.00 0.00627 10800.00 10000.00 0.00 0.00709 11000.00 10000.00 0.00 0.00798 11200.00 10000.00 0.00 0.00901 11400.00 10000.00 0.00 0.01025 11600.00 10000.00 0.00 0.01144 11800.00 10000.00 0.00 0.01253 12000.00 10000.00 0.00 0.01365 12200.00 10000.00 0.00 0.01472 12400.00 10000.00 0.00 0.01596 12600.00 10000.00 0.00 0.01735 12800.00 10000.00 0.00 0.01879 13000.00 10000.00 0.00 0.02049 13200.00 10000.00 0.00 0.02225 13400.00 10000.00 0.00 0.02394 13600.00 10000.00 0.00 0.02572 13800.00 10000.00 0.00 0.02767 14000.00 10000.00 0.00 0.02965 14200.00 10000.00 0.00 0.03177 14400.00 10000.00 0.00 0.03394 14600.00 10000.00 0.00 0.03612 14800.00 10000.00 0.00 0.03825 15000.00 10000.00 0.00 0.04038 15200.00 10000.00 0.00 0.04271 15400.00 10000.00 0.00 0.04510 15600.00 10000.00 0.00 0.04775 15800.00 10000.00 0.00 0.05065 16000.00 10000.00 0.00 0.05378 16200.00 10000.00 0.00 0.05698 16400.00 10000.00 0.00 0.06022 16600.00 10000.00 0.00 0.06363 16800.00 10000.00 0.00 0.06611 17000.00 10000.00 0.00 0.06714 17200.00 10000.00 0.00 0.06724 17000.00 10200.00 0.00 0.06806 17200.00 10200.00 0.00 0.06819 17000.00 10400.00 0.00 0.06905 17200.00 10400.00 0.00 0.06928 17000.00 10600.00 0.00 0.07036 17200.00 10600.00 0.00 0.07063 17000.00 10800.00 0.00 0.07167 17200.00 10800.00 0.00 0.07192 16800.00 11000.00 0.00 0.07212 17000.00 11000.00 0.00 0.07323 17200.00 11000.00 0.00 0.07346 16800.00 11200.00 0.00 0.07370 17000.00 11200.00 0.00 0.07484 17200.00 11200.00 0.00 0.07506 16800.00 11400.00 0.00 0.07535 17000.00 11400.00 0.00 0.07654 17200.00 11400.00 0.00 0.07670 16800.00 11600.00 0.00 0.07704 17000.00 11600.00 0.00 0.07822 17200.00 11600.00 0.00 0.07831 16800.00 11800.00 0.00 0.07887 17000.00 11800.00 0.00 0.08000 17200.00 11800.00 0.00 0.08003 16800.00 12000.00 0.00 0.08075 17000.00 12000.00 0.00 0.08185 17200.00 12000.00 0.00 0.08186 16800.00 12200.00 0.00 0.08266 17000.00 12200.00 0.00 0.08377 17200.00 12200.00 0.00 0.08380 16800.00 12400.00 0.00 0.08483 17000.00 12400.00 0.00 0.08594 17200.00 12400.00 0.00 0.08594 16800.00 12600.00 0.00 0.08691 17000.00 12600.00 0.00 0.08799 16800.00 12800.00 0.00 0.08905 17000.00 12800.00 0.00 0.09014 16800.00 13000.00 0.00 0.09136 17000.00 13000.00 0.00 0.09245 16800.00 13200.00 0.00 0.09389 17000.00 13200.00 0.00 0.09495 16800.00 13400.00 0.00 0.09646 17000.00 13400.00 0.00 0.09755 16800.00 13600.00 0.00 0.09921 17000.00 13600.00 0.00 0.10032 16800.00 13800.00 0.00 0.10180 17000.00 13800.00 0.00 0.10287 16800.00 14000.00 0.00 0.10423 17000.00 14000.00 0.00 0.10530 16800.00 14200.00 0.00 0.10715 17000.00 14200.00 0.00 0.10820 16800.00 14400.00 0.00 0.11002 17000.00 14400.00 0.00 0.11098 16800.00 14600.00 0.00 0.11308 17000.00 14600.00 0.00 0.11392 16800.00 14800.00 0.00 0.11621 17000.00 14800.00 0.00 0.11693 16800.00 15000.00 0.00 0.11951 17000.00 15000.00 0.00 0.12008 16600.00 15200.00 0.00 0.12029 16800.00 15200.00 0.00 0.12296 17000.00 15200.00 0.00 0.12340 16600.00 15400.00 0.00 0.12401 16800.00 15400.00 0.00 0.12665 17000.00 15400.00 0.00 0.12690 16600.00 15600.00 0.00 0.12804 16800.00 15600.00 0.00 0.13057 16600.00 15800.00 0.00 0.13189 16800.00 15800.00 0.00 0.13427 16600.00 16000.00 0.00 0.13626 16800.00 16000.00 0.00 0.13835 16600.00 16200.00 0.00 0.14039 16800.00 16200.00 0.00 0.14213 16600.00 16400.00 0.00 0.14493 16800.00 16400.00 0.00 0.14597 16600.00 16600.00 0.00 0.14906 16600.00 16800.00 0.00 0.15009 16600.00 16800.00 5.00 0.15024 16600.00 16800.00 10.00 0.15037 16600.00 16800.00 15.00 0.15047 16600.00 16800.00 20.00 0.15054 16600.00 16800.00 25.00 0.15057 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16609.98 16810.93 27.51 0.15059 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/464.ctf NX, NY, NZ: 512 512 1 MODE : real