CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/460.mrc Output diagnostic file name Micrographs_0601/460.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/460.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2238.000 8008.000 Mean, RMS : 4469.932 216.5546 TITLE 1: emi2mrc: 460.emi Mon Jan 06 15:28:27 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 18.878, Y 478.702, Z -31.721, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 61 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01361 10200.00 10000.00 0.00 0.01442 10400.00 10000.00 0.00 0.01526 10600.00 10000.00 0.00 0.01635 10800.00 10000.00 0.00 0.01760 11000.00 10000.00 0.00 0.01906 11200.00 10000.00 0.00 0.02043 11400.00 10000.00 0.00 0.02187 11600.00 10000.00 0.00 0.02369 11800.00 10000.00 0.00 0.02561 12000.00 10000.00 0.00 0.02772 12200.00 10000.00 0.00 0.02982 12400.00 10000.00 0.00 0.03195 12600.00 10000.00 0.00 0.03438 12800.00 10000.00 0.00 0.03705 13000.00 10000.00 0.00 0.03993 13200.00 10000.00 0.00 0.04273 13400.00 10000.00 0.00 0.04532 13600.00 10000.00 0.00 0.04813 13800.00 10000.00 0.00 0.05118 14000.00 10000.00 0.00 0.05405 14200.00 10000.00 0.00 0.05705 14400.00 10000.00 0.00 0.06016 14600.00 10000.00 0.00 0.06352 14800.00 10000.00 0.00 0.06726 15000.00 10000.00 0.00 0.07116 15200.00 10000.00 0.00 0.07502 15400.00 10000.00 0.00 0.07764 15600.00 10000.00 0.00 0.07868 15800.00 10000.00 0.00 0.07881 15400.00 10200.00 0.00 0.07941 15600.00 10200.00 0.00 0.08038 15800.00 10200.00 0.00 0.08047 15400.00 10400.00 0.00 0.08128 15600.00 10400.00 0.00 0.08224 15800.00 10400.00 0.00 0.08234 15400.00 10600.00 0.00 0.08352 15600.00 10600.00 0.00 0.08448 15800.00 10600.00 0.00 0.08457 15400.00 10800.00 0.00 0.08601 15600.00 10800.00 0.00 0.08690 15400.00 11000.00 0.00 0.08844 15600.00 11000.00 0.00 0.08923 15400.00 11200.00 0.00 0.09058 15600.00 11200.00 0.00 0.09131 15400.00 11400.00 0.00 0.09302 15600.00 11400.00 0.00 0.09369 15400.00 11600.00 0.00 0.09534 15600.00 11600.00 0.00 0.09589 15400.00 11800.00 0.00 0.09781 15600.00 11800.00 0.00 0.09829 15400.00 12000.00 0.00 0.10046 15600.00 12000.00 0.00 0.10088 15400.00 12200.00 0.00 0.10319 15600.00 12200.00 0.00 0.10359 15200.00 12400.00 0.00 0.10381 15400.00 12400.00 0.00 0.10628 15600.00 12400.00 0.00 0.10662 15200.00 12600.00 0.00 0.10689 15400.00 12600.00 0.00 0.10929 15600.00 12600.00 0.00 0.10952 15200.00 12800.00 0.00 0.11022 15400.00 12800.00 0.00 0.11256 15600.00 12800.00 0.00 0.11269 15200.00 13000.00 0.00 0.11342 15400.00 13000.00 0.00 0.11573 15600.00 13000.00 0.00 0.11576 15200.00 13200.00 0.00 0.11682 15400.00 13200.00 0.00 0.11908 15200.00 13400.00 0.00 0.12029 15400.00 13400.00 0.00 0.12247 15200.00 13600.00 0.00 0.12373 15400.00 13600.00 0.00 0.12579 15200.00 13800.00 0.00 0.12714 15400.00 13800.00 0.00 0.12903 15200.00 14000.00 0.00 0.13037 15400.00 14000.00 0.00 0.13204 15200.00 14200.00 0.00 0.13391 15400.00 14200.00 0.00 0.13532 15200.00 14400.00 0.00 0.13725 15400.00 14400.00 0.00 0.13839 15200.00 14600.00 0.00 0.14108 15400.00 14600.00 0.00 0.14189 15200.00 14800.00 0.00 0.14459 15400.00 14800.00 0.00 0.14488 15200.00 15000.00 0.00 0.14787 15200.00 15200.00 0.00 0.15060 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15194.89 15281.26 27.06 0.15090 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/460.ctf NX, NY, NZ: 512 512 1 MODE : real