CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/456.mrc Output diagnostic file name Micrographs_0601/456.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/456.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2472.000 7668.000 Mean, RMS : 4481.492 212.1153 TITLE 1: emi2mrc: 456.emi Mon Jan 06 15:23:56 2014 TITLE 2: kV 300, Mag 78000x, Def 19030, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 12.098, Y 482.665, Z -30.602, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02266 10200.00 10000.00 0.00 0.02439 10400.00 10000.00 0.00 0.02576 10600.00 10000.00 0.00 0.02727 10800.00 10000.00 0.00 0.02890 11000.00 10000.00 0.00 0.03081 11200.00 10000.00 0.00 0.03268 11400.00 10000.00 0.00 0.03452 11600.00 10000.00 0.00 0.03676 11800.00 10000.00 0.00 0.03903 12000.00 10000.00 0.00 0.04140 12200.00 10000.00 0.00 0.04385 12400.00 10000.00 0.00 0.04639 12600.00 10000.00 0.00 0.04914 12800.00 10000.00 0.00 0.05225 13000.00 10000.00 0.00 0.05552 13200.00 10000.00 0.00 0.05873 13400.00 10000.00 0.00 0.06179 13600.00 10000.00 0.00 0.06470 13800.00 10000.00 0.00 0.06799 14000.00 10000.00 0.00 0.07133 14200.00 10000.00 0.00 0.07466 14400.00 10000.00 0.00 0.07776 14600.00 10000.00 0.00 0.07991 14800.00 10000.00 0.00 0.08099 15000.00 10000.00 0.00 0.08150 15200.00 10000.00 0.00 0.08162 14600.00 10200.00 0.00 0.08196 14800.00 10200.00 0.00 0.08307 15000.00 10200.00 0.00 0.08357 15200.00 10200.00 0.00 0.08365 14600.00 10400.00 0.00 0.08414 14800.00 10400.00 0.00 0.08524 15000.00 10400.00 0.00 0.08568 15200.00 10400.00 0.00 0.08570 14600.00 10600.00 0.00 0.08644 14800.00 10600.00 0.00 0.08746 15000.00 10600.00 0.00 0.08784 15200.00 10600.00 0.00 0.08784 14600.00 10800.00 0.00 0.08864 14800.00 10800.00 0.00 0.08961 15000.00 10800.00 0.00 0.08998 15200.00 10800.00 0.00 0.09000 14600.00 11000.00 0.00 0.09095 14800.00 11000.00 0.00 0.09195 15000.00 11000.00 0.00 0.09230 14600.00 11200.00 0.00 0.09327 14800.00 11200.00 0.00 0.09425 15000.00 11200.00 0.00 0.09449 14600.00 11400.00 0.00 0.09576 14800.00 11400.00 0.00 0.09667 15000.00 11400.00 0.00 0.09681 14600.00 11600.00 0.00 0.09821 14800.00 11600.00 0.00 0.09912 15000.00 11600.00 0.00 0.09919 14600.00 11800.00 0.00 0.10091 14800.00 11800.00 0.00 0.10181 14600.00 12000.00 0.00 0.10363 14800.00 12000.00 0.00 0.10447 14400.00 12200.00 0.00 0.10454 14600.00 12200.00 0.00 0.10648 14800.00 12200.00 0.00 0.10720 14400.00 12400.00 0.00 0.10762 14600.00 12400.00 0.00 0.10946 14800.00 12400.00 0.00 0.10999 14400.00 12600.00 0.00 0.11046 14600.00 12600.00 0.00 0.11216 14800.00 12600.00 0.00 0.11252 14400.00 12800.00 0.00 0.11336 14600.00 12800.00 0.00 0.11491 14800.00 12800.00 0.00 0.11511 14400.00 13000.00 0.00 0.11591 14600.00 13000.00 0.00 0.11728 14800.00 13000.00 0.00 0.11734 14400.00 13200.00 0.00 0.11859 14600.00 13200.00 0.00 0.11980 14400.00 13400.00 0.00 0.12114 14600.00 13400.00 0.00 0.12220 14400.00 13600.00 0.00 0.12371 14600.00 13600.00 0.00 0.12456 14400.00 13800.00 0.00 0.12604 14600.00 13800.00 0.00 0.12662 14400.00 14000.00 0.00 0.12797 14600.00 14000.00 0.00 0.12837 14200.00 14200.00 0.00 0.12860 14400.00 14200.00 0.00 0.13043 14600.00 14200.00 0.00 0.13052 14400.00 14400.00 0.00 0.13159 14400.00 14600.00 0.00 0.13207 14400.00 14600.00 5.00 0.13207 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14449.53 14640.54 -3.19 0.13212 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/456.ctf NX, NY, NZ: 512 512 1 MODE : real