CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/455.mrc Output diagnostic file name Micrographs_0601/455.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/455.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2320.000 8452.000 Mean, RMS : 4496.398 223.7683 TITLE 1: emi2mrc: 455.emi Mon Jan 06 15:22:41 2014 TITLE 2: kV 300, Mag 78000x, Def 22320, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 10.567, Y 485.096, Z -30.602, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01960 10200.00 10000.00 0.00 -0.01955 10400.00 10000.00 0.00 -0.01934 10600.00 10000.00 0.00 -0.01913 10800.00 10000.00 0.00 -0.01890 11000.00 10000.00 0.00 -0.01871 11200.00 10000.00 0.00 -0.01840 11400.00 10000.00 0.00 -0.01798 11600.00 10000.00 0.00 -0.01773 11800.00 10000.00 0.00 -0.01754 12000.00 10000.00 0.00 -0.01726 12200.00 10000.00 0.00 -0.01702 12400.00 10000.00 0.00 -0.01682 12600.00 10000.00 0.00 -0.01654 12800.00 10000.00 0.00 -0.01623 13000.00 10000.00 0.00 -0.01585 13200.00 10000.00 0.00 -0.01542 13400.00 10000.00 0.00 -0.01498 13600.00 10000.00 0.00 -0.01460 13800.00 10000.00 0.00 -0.01435 14000.00 10000.00 0.00 -0.01415 14200.00 10000.00 0.00 -0.01391 14400.00 10000.00 0.00 -0.01357 14600.00 10000.00 0.00 -0.01316 14800.00 10000.00 0.00 -0.01261 15000.00 10000.00 0.00 -0.01192 15200.00 10000.00 0.00 -0.01114 15400.00 10000.00 0.00 -0.01040 15600.00 10000.00 0.00 -0.00969 15800.00 10000.00 0.00 -0.00900 16000.00 10000.00 0.00 -0.00846 16200.00 10000.00 0.00 -0.00776 16400.00 10000.00 0.00 -0.00687 16600.00 10000.00 0.00 -0.00596 16800.00 10000.00 0.00 -0.00489 17000.00 10000.00 0.00 -0.00372 17200.00 10000.00 0.00 -0.00258 17400.00 10000.00 0.00 -0.00149 17600.00 10000.00 0.00 -0.00033 17800.00 10000.00 0.00 0.00083 18000.00 10000.00 0.00 0.00191 18200.00 10000.00 0.00 0.00292 18400.00 10000.00 0.00 0.00394 18600.00 10000.00 0.00 0.00512 18800.00 10000.00 0.00 0.00637 19000.00 10000.00 0.00 0.00739 19200.00 10000.00 0.00 0.00828 19400.00 10000.00 0.00 0.00935 19600.00 10000.00 0.00 0.01060 19800.00 10000.00 0.00 0.01193 20000.00 10000.00 0.00 0.01323 20200.00 10000.00 0.00 0.01450 20400.00 10000.00 0.00 0.01577 20600.00 10000.00 0.00 0.01708 20800.00 10000.00 0.00 0.01850 21000.00 10000.00 0.00 0.01999 21200.00 10000.00 0.00 0.02154 21400.00 10000.00 0.00 0.02316 21600.00 10000.00 0.00 0.02498 21800.00 10000.00 0.00 0.02699 22000.00 10000.00 0.00 0.02899 22200.00 10000.00 0.00 0.03083 22400.00 10000.00 0.00 0.03250 22600.00 10000.00 0.00 0.03390 22800.00 10000.00 0.00 0.03482 23000.00 10000.00 0.00 0.03538 23200.00 10000.00 0.00 0.03567 23400.00 10000.00 0.00 0.03574 23200.00 10200.00 0.00 0.03590 23400.00 10200.00 0.00 0.03601 23200.00 10400.00 0.00 0.03630 23400.00 10400.00 0.00 0.03647 23600.00 10400.00 0.00 0.03649 23800.00 10400.00 0.00 0.03653 23200.00 10600.00 0.00 0.03689 23400.00 10600.00 0.00 0.03706 23600.00 10600.00 0.00 0.03708 23800.00 10600.00 0.00 0.03710 23200.00 10800.00 0.00 0.03744 23400.00 10800.00 0.00 0.03759 23600.00 10800.00 0.00 0.03761 23800.00 10800.00 0.00 0.03764 23000.00 11000.00 0.00 0.03766 23200.00 11000.00 0.00 0.03802 23400.00 11000.00 0.00 0.03821 23600.00 11000.00 0.00 0.03828 23800.00 11000.00 0.00 0.03831 23200.00 11200.00 0.00 0.03871 23400.00 11200.00 0.00 0.03895 23600.00 11200.00 0.00 0.03902 23000.00 11400.00 0.00 0.03906 23200.00 11400.00 0.00 0.03949 23400.00 11400.00 0.00 0.03972 23200.00 11600.00 0.00 0.04017 23400.00 11600.00 0.00 0.04037 23000.00 11800.00 0.00 0.04041 23200.00 11800.00 0.00 0.04086 23400.00 11800.00 0.00 0.04102 23000.00 12000.00 0.00 0.04112 23200.00 12000.00 0.00 0.04153 23400.00 12000.00 0.00 0.04162 23000.00 12200.00 0.00 0.04170 23200.00 12200.00 0.00 0.04208 23400.00 12200.00 0.00 0.04214 23000.00 12400.00 0.00 0.04236 23200.00 12400.00 0.00 0.04272 23400.00 12400.00 0.00 0.04276 23000.00 12600.00 0.00 0.04305 23200.00 12600.00 0.00 0.04337 23000.00 12800.00 0.00 0.04366 23200.00 12800.00 0.00 0.04393 23000.00 13000.00 0.00 0.04430 23200.00 13000.00 0.00 0.04455 23000.00 13200.00 0.00 0.04504 23200.00 13200.00 0.00 0.04530 23400.00 13200.00 0.00 0.04533 23000.00 13400.00 0.00 0.04579 23200.00 13400.00 0.00 0.04609 23400.00 13400.00 0.00 0.04613 23000.00 13600.00 0.00 0.04670 23200.00 13600.00 0.00 0.04704 23400.00 13600.00 0.00 0.04709 23000.00 13800.00 0.00 0.04775 23200.00 13800.00 0.00 0.04811 22800.00 14000.00 0.00 0.04815 23000.00 14000.00 0.00 0.04886 23200.00 14000.00 0.00 0.04918 22800.00 14200.00 0.00 0.04932 23000.00 14200.00 0.00 0.05000 23200.00 14200.00 0.00 0.05026 22800.00 14400.00 0.00 0.05041 23000.00 14400.00 0.00 0.05106 23200.00 14400.00 0.00 0.05129 22800.00 14600.00 0.00 0.05158 23000.00 14600.00 0.00 0.05223 23200.00 14600.00 0.00 0.05246 22800.00 14800.00 0.00 0.05271 23000.00 14800.00 0.00 0.05336 23200.00 14800.00 0.00 0.05358 22800.00 15000.00 0.00 0.05382 23000.00 15000.00 0.00 0.05445 23200.00 15000.00 0.00 0.05464 22800.00 15200.00 0.00 0.05491 23000.00 15200.00 0.00 0.05550 23200.00 15200.00 0.00 0.05565 22800.00 15400.00 0.00 0.05596 23000.00 15400.00 0.00 0.05654 23200.00 15400.00 0.00 0.05666 22800.00 15600.00 0.00 0.05711 23000.00 15600.00 0.00 0.05769 23200.00 15600.00 0.00 0.05777 22800.00 15800.00 0.00 0.05831 23000.00 15800.00 0.00 0.05889 23200.00 15800.00 0.00 0.05894 22800.00 16000.00 0.00 0.05962 23000.00 16000.00 0.00 0.06017 23200.00 16000.00 0.00 0.06017 22800.00 16200.00 0.00 0.06099 23000.00 16200.00 0.00 0.06148 22800.00 16400.00 0.00 0.06246 23000.00 16400.00 0.00 0.06290 22800.00 16600.00 0.00 0.06382 23000.00 16600.00 0.00 0.06421 22600.00 16800.00 0.00 0.06422 22800.00 16800.00 0.00 0.06517 23000.00 16800.00 0.00 0.06552 22600.00 17000.00 0.00 0.06572 22800.00 17000.00 0.00 0.06665 23000.00 17000.00 0.00 0.06700 22600.00 17200.00 0.00 0.06722 22800.00 17200.00 0.00 0.06812 23000.00 17200.00 0.00 0.06847 22600.00 17400.00 0.00 0.06876 22800.00 17400.00 0.00 0.06963 23000.00 17400.00 0.00 0.06998 22600.00 17600.00 0.00 0.07025 22800.00 17600.00 0.00 0.07108 23000.00 17600.00 0.00 0.07141 22600.00 17800.00 0.00 0.07176 22800.00 17800.00 0.00 0.07257 23000.00 17800.00 0.00 0.07289 22600.00 18000.00 0.00 0.07326 22800.00 18000.00 0.00 0.07407 23000.00 18000.00 0.00 0.07438 22600.00 18200.00 0.00 0.07495 22800.00 18200.00 0.00 0.07576 23000.00 18200.00 0.00 0.07606 22600.00 18400.00 0.00 0.07669 22800.00 18400.00 0.00 0.07750 23000.00 18400.00 0.00 0.07778 22600.00 18600.00 0.00 0.07838 22800.00 18600.00 0.00 0.07920 23000.00 18600.00 0.00 0.07948 22600.00 18800.00 0.00 0.08025 22800.00 18800.00 0.00 0.08109 23000.00 18800.00 0.00 0.08132 22600.00 19000.00 0.00 0.08202 22800.00 19000.00 0.00 0.08282 23000.00 19000.00 0.00 0.08298 22600.00 19200.00 0.00 0.08368 22800.00 19200.00 0.00 0.08445 23000.00 19200.00 0.00 0.08453 22600.00 19400.00 0.00 0.08532 22800.00 19400.00 0.00 0.08604 23000.00 19400.00 0.00 0.08608 22600.00 19600.00 0.00 0.08705 22800.00 19600.00 0.00 0.08773 23000.00 19600.00 0.00 0.08773 22600.00 19800.00 0.00 0.08887 22800.00 19800.00 0.00 0.08953 22600.00 20000.00 0.00 0.09092 22800.00 20000.00 0.00 0.09155 22600.00 20200.00 0.00 0.09300 22800.00 20200.00 0.00 0.09356 22600.00 20400.00 0.00 0.09494 22800.00 20400.00 0.00 0.09540 22400.00 20600.00 0.00 0.09560 22600.00 20600.00 0.00 0.09702 22800.00 20600.00 0.00 0.09734 22400.00 20800.00 0.00 0.09766 22600.00 20800.00 0.00 0.09899 22800.00 20800.00 0.00 0.09918 22400.00 21000.00 0.00 0.09987 22600.00 21000.00 0.00 0.10111 22800.00 21000.00 0.00 0.10117 22400.00 21200.00 0.00 0.10203 22600.00 21200.00 0.00 0.10316 22400.00 21400.00 0.00 0.10423 22600.00 21400.00 0.00 0.10521 22400.00 21600.00 0.00 0.10640 22600.00 21600.00 0.00 0.10719 22400.00 21800.00 0.00 0.10836 22600.00 21800.00 0.00 0.10897 22400.00 22000.00 0.00 0.11035 22600.00 22000.00 0.00 0.11074 22400.00 22200.00 0.00 0.11196 22600.00 22200.00 0.00 0.11207 22200.00 22400.00 0.00 0.11210 22400.00 22400.00 0.00 0.11335 22400.00 22600.00 0.00 0.11356 22400.00 22600.00 5.00 0.11359 22400.00 22600.00 10.00 0.11362 22400.00 22600.00 15.00 0.11364 22400.00 22600.00 20.00 0.11365 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22406.41 22529.78 21.15 0.11377 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/455.ctf NX, NY, NZ: 512 512 1 MODE : real