CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/454.mrc Output diagnostic file name Micrographs_0601/454.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/454.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2080.000 9004.000 Mean, RMS : 4477.668 216.0960 TITLE 1: emi2mrc: 454.emi Mon Jan 06 15:16:08 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 226.081, Y 441.365, Z -38.277, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01564 10200.00 10000.00 0.00 0.01692 10400.00 10000.00 0.00 0.01803 10600.00 10000.00 0.00 0.01903 10800.00 10000.00 0.00 0.02022 11000.00 10000.00 0.00 0.02158 11200.00 10000.00 0.00 0.02304 11400.00 10000.00 0.00 0.02483 11600.00 10000.00 0.00 0.02664 11800.00 10000.00 0.00 0.02846 12000.00 10000.00 0.00 0.03044 12200.00 10000.00 0.00 0.03242 12400.00 10000.00 0.00 0.03482 12600.00 10000.00 0.00 0.03731 12800.00 10000.00 0.00 0.03974 13000.00 10000.00 0.00 0.04235 13200.00 10000.00 0.00 0.04514 13400.00 10000.00 0.00 0.04801 13600.00 10000.00 0.00 0.05073 13800.00 10000.00 0.00 0.05361 14000.00 10000.00 0.00 0.05671 14200.00 10000.00 0.00 0.06003 14400.00 10000.00 0.00 0.06347 14600.00 10000.00 0.00 0.06724 14800.00 10000.00 0.00 0.07138 15000.00 10000.00 0.00 0.07538 15200.00 10000.00 0.00 0.07882 15400.00 10000.00 0.00 0.08061 15600.00 10000.00 0.00 0.08094 15400.00 10200.00 0.00 0.08244 15600.00 10200.00 0.00 0.08280 15200.00 10400.00 0.00 0.08281 15400.00 10400.00 0.00 0.08465 15600.00 10400.00 0.00 0.08498 15400.00 10600.00 0.00 0.08671 15600.00 10600.00 0.00 0.08696 15400.00 10800.00 0.00 0.08850 15600.00 10800.00 0.00 0.08871 15200.00 11000.00 0.00 0.08881 15400.00 11000.00 0.00 0.09056 15600.00 11000.00 0.00 0.09074 15200.00 11200.00 0.00 0.09094 15400.00 11200.00 0.00 0.09268 15600.00 11200.00 0.00 0.09281 15200.00 11400.00 0.00 0.09334 15400.00 11400.00 0.00 0.09503 15600.00 11400.00 0.00 0.09506 15200.00 11600.00 0.00 0.09562 15400.00 11600.00 0.00 0.09724 15200.00 11800.00 0.00 0.09805 15400.00 11800.00 0.00 0.09963 15200.00 12000.00 0.00 0.10065 15400.00 12000.00 0.00 0.10216 15200.00 12200.00 0.00 0.10320 15400.00 12200.00 0.00 0.10459 15200.00 12400.00 0.00 0.10584 15400.00 12400.00 0.00 0.10715 15200.00 12600.00 0.00 0.10847 15400.00 12600.00 0.00 0.10979 15200.00 12800.00 0.00 0.11138 15400.00 12800.00 0.00 0.11270 15200.00 13000.00 0.00 0.11430 15400.00 13000.00 0.00 0.11560 15200.00 13200.00 0.00 0.11761 15400.00 13200.00 0.00 0.11884 15200.00 13400.00 0.00 0.12092 15400.00 13400.00 0.00 0.12204 15200.00 13600.00 0.00 0.12444 15400.00 13600.00 0.00 0.12543 15200.00 13800.00 0.00 0.12808 15400.00 13800.00 0.00 0.12886 15200.00 14000.00 0.00 0.13138 15400.00 14000.00 0.00 0.13195 15200.00 14200.00 0.00 0.13509 15400.00 14200.00 0.00 0.13539 15200.00 14400.00 0.00 0.13860 15000.00 14600.00 0.00 0.13883 15200.00 14600.00 0.00 0.14231 15200.00 14800.00 0.00 0.14546 15200.00 15000.00 0.00 0.14843 15000.00 15200.00 0.00 0.14866 15200.00 15200.00 0.00 0.15085 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15141.08 15307.67 20.36 0.15135 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/454.ctf NX, NY, NZ: 512 512 1 MODE : real