CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/452.mrc Output diagnostic file name Micrographs_0601/452.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/452.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2334.000 7656.000 Mean, RMS : 4485.820 220.5978 TITLE 1: emi2mrc: 452.emi Mon Jan 06 15:13:17 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 222.908, Y 446.101, Z -38.075, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02160 11600.00 10000.00 0.00 -0.02150 11800.00 10000.00 0.00 -0.02134 12000.00 10000.00 0.00 -0.02120 12200.00 10000.00 0.00 -0.02085 12400.00 10000.00 0.00 -0.02041 12600.00 10000.00 0.00 -0.01999 12800.00 10000.00 0.00 -0.01971 13000.00 10000.00 0.00 -0.01949 13200.00 10000.00 0.00 -0.01908 13400.00 10000.00 0.00 -0.01861 13600.00 10000.00 0.00 -0.01812 13800.00 10000.00 0.00 -0.01758 14000.00 10000.00 0.00 -0.01698 14200.00 10000.00 0.00 -0.01637 14400.00 10000.00 0.00 -0.01580 14600.00 10000.00 0.00 -0.01526 14800.00 10000.00 0.00 -0.01467 15000.00 10000.00 0.00 -0.01397 15200.00 10000.00 0.00 -0.01329 15400.00 10000.00 0.00 -0.01271 15600.00 10000.00 0.00 -0.01203 15800.00 10000.00 0.00 -0.01124 16000.00 10000.00 0.00 -0.01048 16200.00 10000.00 0.00 -0.00947 16400.00 10000.00 0.00 -0.00827 16600.00 10000.00 0.00 -0.00726 16800.00 10000.00 0.00 -0.00624 17000.00 10000.00 0.00 -0.00516 17200.00 10000.00 0.00 -0.00429 17400.00 10000.00 0.00 -0.00336 17600.00 10000.00 0.00 -0.00224 17800.00 10000.00 0.00 -0.00117 18000.00 10000.00 0.00 -0.00014 18200.00 10000.00 0.00 0.00096 18400.00 10000.00 0.00 0.00215 18600.00 10000.00 0.00 0.00333 18800.00 10000.00 0.00 0.00445 19000.00 10000.00 0.00 0.00556 19200.00 10000.00 0.00 0.00660 19400.00 10000.00 0.00 0.00765 19600.00 10000.00 0.00 0.00865 19800.00 10000.00 0.00 0.00957 20000.00 10000.00 0.00 0.01063 20200.00 10000.00 0.00 0.01173 20400.00 10000.00 0.00 0.01289 20600.00 10000.00 0.00 0.01434 20800.00 10000.00 0.00 0.01580 21000.00 10000.00 0.00 0.01725 21200.00 10000.00 0.00 0.01884 21400.00 10000.00 0.00 0.02031 21600.00 10000.00 0.00 0.02179 21800.00 10000.00 0.00 0.02355 22000.00 10000.00 0.00 0.02536 22200.00 10000.00 0.00 0.02720 22400.00 10000.00 0.00 0.02883 22600.00 10000.00 0.00 0.02994 22800.00 10000.00 0.00 0.03075 23000.00 10000.00 0.00 0.03132 23200.00 10000.00 0.00 0.03178 23400.00 10000.00 0.00 0.03216 23600.00 10000.00 0.00 0.03222 23800.00 10000.00 0.00 0.03222 24000.00 10000.00 0.00 0.03234 24200.00 10000.00 0.00 0.03261 24400.00 10000.00 0.00 0.03276 24000.00 10200.00 0.00 0.03286 24200.00 10200.00 0.00 0.03316 24400.00 10200.00 0.00 0.03332 24000.00 10400.00 0.00 0.03344 24200.00 10400.00 0.00 0.03374 24400.00 10400.00 0.00 0.03387 24000.00 10600.00 0.00 0.03393 24200.00 10600.00 0.00 0.03420 24400.00 10600.00 0.00 0.03426 23600.00 10800.00 0.00 0.03427 23800.00 10800.00 0.00 0.03435 24000.00 10800.00 0.00 0.03454 24200.00 10800.00 0.00 0.03477 24400.00 10800.00 0.00 0.03478 23400.00 11000.00 0.00 0.03493 23600.00 11000.00 0.00 0.03500 23800.00 11000.00 0.00 0.03510 24000.00 11000.00 0.00 0.03529 24200.00 11000.00 0.00 0.03547 23400.00 11200.00 0.00 0.03562 23600.00 11200.00 0.00 0.03572 23800.00 11200.00 0.00 0.03582 24000.00 11200.00 0.00 0.03595 24200.00 11200.00 0.00 0.03609 23400.00 11400.00 0.00 0.03646 23600.00 11400.00 0.00 0.03653 23800.00 11400.00 0.00 0.03657 24000.00 11400.00 0.00 0.03665 24200.00 11400.00 0.00 0.03676 23200.00 11600.00 0.00 0.03701 23400.00 11600.00 0.00 0.03736 24200.00 11600.00 0.00 0.03748 23200.00 11800.00 0.00 0.03793 23400.00 11800.00 0.00 0.03815 23200.00 12000.00 0.00 0.03857 23400.00 12000.00 0.00 0.03870 23000.00 12200.00 0.00 0.03872 23200.00 12200.00 0.00 0.03917 23400.00 12200.00 0.00 0.03926 23000.00 12400.00 0.00 0.03946 23200.00 12400.00 0.00 0.03988 23400.00 12400.00 0.00 0.03993 23000.00 12600.00 0.00 0.04024 23200.00 12600.00 0.00 0.04058 23400.00 12600.00 0.00 0.04061 23000.00 12800.00 0.00 0.04109 23200.00 12800.00 0.00 0.04136 23400.00 12800.00 0.00 0.04138 24200.00 12800.00 0.00 0.04147 23000.00 13000.00 0.00 0.04175 23200.00 13000.00 0.00 0.04202 23400.00 13000.00 0.00 0.04210 23800.00 13000.00 0.00 0.04211 24000.00 13000.00 0.00 0.04222 24200.00 13000.00 0.00 0.04236 23000.00 13200.00 0.00 0.04254 23200.00 13200.00 0.00 0.04286 23400.00 13200.00 0.00 0.04302 23600.00 13200.00 0.00 0.04304 23800.00 13200.00 0.00 0.04308 24000.00 13200.00 0.00 0.04319 24200.00 13200.00 0.00 0.04329 23000.00 13400.00 0.00 0.04357 23200.00 13400.00 0.00 0.04393 23400.00 13400.00 0.00 0.04410 23600.00 13400.00 0.00 0.04411 23800.00 13400.00 0.00 0.04412 24000.00 13400.00 0.00 0.04419 24200.00 13400.00 0.00 0.04424 23000.00 13600.00 0.00 0.04456 23200.00 13600.00 0.00 0.04493 23400.00 13600.00 0.00 0.04511 24000.00 13600.00 0.00 0.04512 24200.00 13600.00 0.00 0.04516 23000.00 13800.00 0.00 0.04570 23200.00 13800.00 0.00 0.04606 23400.00 13800.00 0.00 0.04621 22800.00 14000.00 0.00 0.04639 23000.00 14000.00 0.00 0.04701 23200.00 14000.00 0.00 0.04730 23400.00 14000.00 0.00 0.04741 22800.00 14200.00 0.00 0.04752 23000.00 14200.00 0.00 0.04807 23200.00 14200.00 0.00 0.04834 23400.00 14200.00 0.00 0.04845 23000.00 14400.00 0.00 0.04893 23200.00 14400.00 0.00 0.04922 23400.00 14400.00 0.00 0.04938 23000.00 14600.00 0.00 0.04990 23200.00 14600.00 0.00 0.05023 23400.00 14600.00 0.00 0.05040 23000.00 14800.00 0.00 0.05091 23200.00 14800.00 0.00 0.05128 23400.00 14800.00 0.00 0.05144 23000.00 15000.00 0.00 0.05204 23200.00 15000.00 0.00 0.05241 23400.00 15000.00 0.00 0.05252 23000.00 15200.00 0.00 0.05308 23200.00 15200.00 0.00 0.05340 23400.00 15200.00 0.00 0.05342 22800.00 15400.00 0.00 0.05350 23000.00 15400.00 0.00 0.05406 23200.00 15400.00 0.00 0.05435 22800.00 15600.00 0.00 0.05463 23000.00 15600.00 0.00 0.05519 23200.00 15600.00 0.00 0.05548 22800.00 15800.00 0.00 0.05576 23000.00 15800.00 0.00 0.05633 23200.00 15800.00 0.00 0.05661 22800.00 16000.00 0.00 0.05708 23000.00 16000.00 0.00 0.05763 23200.00 16000.00 0.00 0.05789 22800.00 16200.00 0.00 0.05837 23000.00 16200.00 0.00 0.05892 23200.00 16200.00 0.00 0.05915 22800.00 16400.00 0.00 0.05966 23000.00 16400.00 0.00 0.06024 23200.00 16400.00 0.00 0.06049 22800.00 16600.00 0.00 0.06111 23000.00 16600.00 0.00 0.06173 23200.00 16600.00 0.00 0.06194 22800.00 16800.00 0.00 0.06253 23000.00 16800.00 0.00 0.06310 23200.00 16800.00 0.00 0.06321 22800.00 17000.00 0.00 0.06365 23000.00 17000.00 0.00 0.06415 23200.00 17000.00 0.00 0.06421 22800.00 17200.00 0.00 0.06481 23000.00 17200.00 0.00 0.06527 23200.00 17200.00 0.00 0.06534 22600.00 17400.00 0.00 0.06541 22800.00 17400.00 0.00 0.06629 23000.00 17400.00 0.00 0.06668 23200.00 17400.00 0.00 0.06671 22600.00 17600.00 0.00 0.06693 22800.00 17600.00 0.00 0.06772 23000.00 17600.00 0.00 0.06806 23200.00 17600.00 0.00 0.06810 22600.00 17800.00 0.00 0.06848 22800.00 17800.00 0.00 0.06922 23000.00 17800.00 0.00 0.06957 23200.00 17800.00 0.00 0.06962 22600.00 18000.00 0.00 0.06991 22800.00 18000.00 0.00 0.07064 23000.00 18000.00 0.00 0.07098 23200.00 18000.00 0.00 0.07102 22600.00 18200.00 0.00 0.07131 22800.00 18200.00 0.00 0.07203 23000.00 18200.00 0.00 0.07236 23200.00 18200.00 0.00 0.07236 22600.00 18400.00 0.00 0.07282 22800.00 18400.00 0.00 0.07350 23000.00 18400.00 0.00 0.07378 22600.00 18600.00 0.00 0.07427 22800.00 18600.00 0.00 0.07490 23000.00 18600.00 0.00 0.07513 22600.00 18800.00 0.00 0.07568 22800.00 18800.00 0.00 0.07628 23000.00 18800.00 0.00 0.07650 22600.00 19000.00 0.00 0.07694 22800.00 19000.00 0.00 0.07753 23000.00 19000.00 0.00 0.07773 22600.00 19200.00 0.00 0.07826 22800.00 19200.00 0.00 0.07882 23000.00 19200.00 0.00 0.07897 22600.00 19400.00 0.00 0.07976 22800.00 19400.00 0.00 0.08028 23000.00 19400.00 0.00 0.08042 22600.00 19600.00 0.00 0.08145 22800.00 19600.00 0.00 0.08194 23000.00 19600.00 0.00 0.08207 22600.00 19800.00 0.00 0.08312 22800.00 19800.00 0.00 0.08359 23000.00 19800.00 0.00 0.08371 22600.00 20000.00 0.00 0.08488 22800.00 20000.00 0.00 0.08532 23000.00 20000.00 0.00 0.08537 22600.00 20200.00 0.00 0.08661 22800.00 20200.00 0.00 0.08697 22400.00 20400.00 0.00 0.08711 22600.00 20400.00 0.00 0.08832 22800.00 20400.00 0.00 0.08859 22400.00 20600.00 0.00 0.08909 22600.00 20600.00 0.00 0.09021 22800.00 20600.00 0.00 0.09036 22400.00 20800.00 0.00 0.09075 22600.00 20800.00 0.00 0.09180 22800.00 20800.00 0.00 0.09186 22400.00 21000.00 0.00 0.09257 22600.00 21000.00 0.00 0.09358 22400.00 21200.00 0.00 0.09449 22600.00 21200.00 0.00 0.09543 22400.00 21400.00 0.00 0.09639 22600.00 21400.00 0.00 0.09716 22400.00 21600.00 0.00 0.09825 22600.00 21600.00 0.00 0.09882 22400.00 21800.00 0.00 0.09989 22600.00 21800.00 0.00 0.10022 22400.00 22000.00 0.00 0.10138 22600.00 22000.00 0.00 0.10138 22400.00 22200.00 0.00 0.10227 22400.00 22400.00 0.00 0.10311 22400.00 22600.00 0.00 0.10322 22400.00 22600.00 5.00 0.10334 22400.00 22600.00 10.00 0.10345 22400.00 22600.00 15.00 0.10353 22400.00 22600.00 20.00 0.10358 22400.00 22600.00 25.00 0.10360 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22344.40 22590.43 25.00 0.10371 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/452.ctf NX, NY, NZ: 512 512 1 MODE : real