CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/451.mrc Output diagnostic file name Micrographs_0601/451.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/451.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2482.000 7958.000 Mean, RMS : 4478.818 222.8008 TITLE 1: emi2mrc: 451.emi Mon Jan 06 14:30:46 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 292.829, Y 850.382, Z -24.919, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01801 13800.00 10000.00 0.00 -0.01780 14000.00 10000.00 0.00 -0.01749 14200.00 10000.00 0.00 -0.01719 14400.00 10000.00 0.00 -0.01685 14600.00 10000.00 0.00 -0.01656 14800.00 10000.00 0.00 -0.01636 15000.00 10000.00 0.00 -0.01616 15200.00 10000.00 0.00 -0.01587 15400.00 10000.00 0.00 -0.01544 15600.00 10000.00 0.00 -0.01487 15800.00 10000.00 0.00 -0.01427 16000.00 10000.00 0.00 -0.01367 16200.00 10000.00 0.00 -0.01316 16400.00 10000.00 0.00 -0.01276 16600.00 10000.00 0.00 -0.01221 16800.00 10000.00 0.00 -0.01150 17000.00 10000.00 0.00 -0.01077 17200.00 10000.00 0.00 -0.01006 17400.00 10000.00 0.00 -0.00934 17600.00 10000.00 0.00 -0.00854 17800.00 10000.00 0.00 -0.00773 18000.00 10000.00 0.00 -0.00682 18200.00 10000.00 0.00 -0.00572 18400.00 10000.00 0.00 -0.00460 18600.00 10000.00 0.00 -0.00360 18800.00 10000.00 0.00 -0.00269 19000.00 10000.00 0.00 -0.00174 19200.00 10000.00 0.00 -0.00078 19400.00 10000.00 0.00 0.00011 19600.00 10000.00 0.00 0.00100 19800.00 10000.00 0.00 0.00200 20000.00 10000.00 0.00 0.00289 20200.00 10000.00 0.00 0.00363 20400.00 10000.00 0.00 0.00459 20600.00 10000.00 0.00 0.00562 20800.00 10000.00 0.00 0.00670 21000.00 10000.00 0.00 0.00795 21200.00 10000.00 0.00 0.00921 21400.00 10000.00 0.00 0.01053 21600.00 10000.00 0.00 0.01173 21800.00 10000.00 0.00 0.01293 22000.00 10000.00 0.00 0.01430 22200.00 10000.00 0.00 0.01577 22400.00 10000.00 0.00 0.01746 22600.00 10000.00 0.00 0.01916 22800.00 10000.00 0.00 0.02086 23000.00 10000.00 0.00 0.02272 23200.00 10000.00 0.00 0.02458 23400.00 10000.00 0.00 0.02641 23600.00 10000.00 0.00 0.02821 23800.00 10000.00 0.00 0.02974 24000.00 10000.00 0.00 0.03056 24200.00 10000.00 0.00 0.03096 24400.00 10000.00 0.00 0.03128 24200.00 10200.00 0.00 0.03132 24400.00 10200.00 0.00 0.03166 24200.00 10400.00 0.00 0.03180 24400.00 10400.00 0.00 0.03216 24200.00 10600.00 0.00 0.03226 24400.00 10600.00 0.00 0.03260 24400.00 10800.00 0.00 0.03288 24400.00 11000.00 0.00 0.03316 24200.00 11200.00 0.00 0.03331 24400.00 11200.00 0.00 0.03359 24200.00 11400.00 0.00 0.03395 24400.00 11400.00 0.00 0.03420 24200.00 11600.00 0.00 0.03469 24400.00 11600.00 0.00 0.03490 24200.00 11800.00 0.00 0.03541 24400.00 11800.00 0.00 0.03556 24200.00 12000.00 0.00 0.03602 24400.00 12000.00 0.00 0.03609 24000.00 12200.00 0.00 0.03612 24200.00 12200.00 0.00 0.03653 24400.00 12200.00 0.00 0.03656 24000.00 12400.00 0.00 0.03660 24200.00 12400.00 0.00 0.03697 24400.00 12400.00 0.00 0.03699 24000.00 12600.00 0.00 0.03700 24200.00 12600.00 0.00 0.03734 24400.00 12600.00 0.00 0.03736 24200.00 12800.00 0.00 0.03761 24400.00 12800.00 0.00 0.03763 24200.00 13000.00 0.00 0.03779 24400.00 13000.00 0.00 0.03781 24000.00 13200.00 0.00 0.03782 24200.00 13200.00 0.00 0.03819 24400.00 13200.00 0.00 0.03820 24000.00 13400.00 0.00 0.03838 24200.00 13400.00 0.00 0.03874 24000.00 13600.00 0.00 0.03895 24200.00 13600.00 0.00 0.03930 24000.00 13800.00 0.00 0.03954 24200.00 13800.00 0.00 0.03988 24000.00 14000.00 0.00 0.04020 24200.00 14000.00 0.00 0.04056 24000.00 14200.00 0.00 0.04098 24200.00 14200.00 0.00 0.04136 24000.00 14400.00 0.00 0.04192 24200.00 14400.00 0.00 0.04229 24000.00 14600.00 0.00 0.04305 24200.00 14600.00 0.00 0.04339 24000.00 14800.00 0.00 0.04410 24200.00 14800.00 0.00 0.04442 24000.00 15000.00 0.00 0.04504 24200.00 15000.00 0.00 0.04533 24000.00 15200.00 0.00 0.04594 24200.00 15200.00 0.00 0.04619 24000.00 15400.00 0.00 0.04692 24200.00 15400.00 0.00 0.04714 23800.00 15600.00 0.00 0.04720 24000.00 15600.00 0.00 0.04805 24200.00 15600.00 0.00 0.04823 23800.00 15800.00 0.00 0.04838 24000.00 15800.00 0.00 0.04916 24200.00 15800.00 0.00 0.04928 23800.00 16000.00 0.00 0.04955 24000.00 16000.00 0.00 0.05027 24200.00 16000.00 0.00 0.05034 23800.00 16200.00 0.00 0.05060 24000.00 16200.00 0.00 0.05127 24200.00 16200.00 0.00 0.05130 23800.00 16400.00 0.00 0.05166 24000.00 16400.00 0.00 0.05230 23800.00 16600.00 0.00 0.05290 24000.00 16600.00 0.00 0.05350 23800.00 16800.00 0.00 0.05417 24000.00 16800.00 0.00 0.05471 23800.00 17000.00 0.00 0.05545 24000.00 17000.00 0.00 0.05595 23800.00 17200.00 0.00 0.05684 24000.00 17200.00 0.00 0.05732 23800.00 17400.00 0.00 0.05829 24000.00 17400.00 0.00 0.05877 23800.00 17600.00 0.00 0.05971 24000.00 17600.00 0.00 0.06017 23800.00 17800.00 0.00 0.06114 24000.00 17800.00 0.00 0.06163 24200.00 17800.00 0.00 0.06168 23800.00 18000.00 0.00 0.06251 24000.00 18000.00 0.00 0.06308 24200.00 18000.00 0.00 0.06318 23800.00 18200.00 0.00 0.06395 24000.00 18200.00 0.00 0.06459 24200.00 18200.00 0.00 0.06465 23800.00 18400.00 0.00 0.06543 24000.00 18400.00 0.00 0.06605 24200.00 18400.00 0.00 0.06606 23800.00 18600.00 0.00 0.06684 24000.00 18600.00 0.00 0.06742 23800.00 18800.00 0.00 0.06829 24000.00 18800.00 0.00 0.06884 24200.00 18800.00 0.00 0.06885 23800.00 19000.00 0.00 0.06978 24000.00 19000.00 0.00 0.07038 24200.00 19000.00 0.00 0.07040 23800.00 19200.00 0.00 0.07135 24000.00 19200.00 0.00 0.07196 24200.00 19200.00 0.00 0.07196 23800.00 19400.00 0.00 0.07284 24000.00 19400.00 0.00 0.07346 23800.00 19600.00 0.00 0.07445 24000.00 19600.00 0.00 0.07509 23800.00 19800.00 0.00 0.07618 24000.00 19800.00 0.00 0.07683 23800.00 20000.00 0.00 0.07797 24000.00 20000.00 0.00 0.07857 23800.00 20200.00 0.00 0.07972 24000.00 20200.00 0.00 0.08028 23800.00 20400.00 0.00 0.08144 24000.00 20400.00 0.00 0.08198 23800.00 20600.00 0.00 0.08335 24000.00 20600.00 0.00 0.08386 23800.00 20800.00 0.00 0.08522 24000.00 20800.00 0.00 0.08570 23800.00 21000.00 0.00 0.08725 24000.00 21000.00 0.00 0.08772 23800.00 21200.00 0.00 0.08944 24000.00 21200.00 0.00 0.08991 23600.00 21400.00 0.00 0.08994 23800.00 21400.00 0.00 0.09172 24000.00 21400.00 0.00 0.09215 23600.00 21600.00 0.00 0.09230 23800.00 21600.00 0.00 0.09410 24000.00 21600.00 0.00 0.09446 23600.00 21800.00 0.00 0.09480 23800.00 21800.00 0.00 0.09659 24000.00 21800.00 0.00 0.09687 23600.00 22000.00 0.00 0.09746 23800.00 22000.00 0.00 0.09924 24000.00 22000.00 0.00 0.09942 23600.00 22200.00 0.00 0.10024 23800.00 22200.00 0.00 0.10198 24000.00 22200.00 0.00 0.10205 23600.00 22400.00 0.00 0.10333 23800.00 22400.00 0.00 0.10503 23600.00 22600.00 0.00 0.10643 23800.00 22600.00 0.00 0.10807 23600.00 22800.00 0.00 0.10975 23800.00 22800.00 0.00 0.11121 23600.00 23000.00 0.00 0.11316 23800.00 23000.00 0.00 0.11436 23600.00 23200.00 0.00 0.11656 23800.00 23200.00 0.00 0.11737 23400.00 23400.00 0.00 0.11744 23600.00 23400.00 0.00 0.11994 23800.00 23400.00 0.00 0.12017 23400.00 23600.00 0.00 0.12060 23600.00 23600.00 0.00 0.12254 23600.00 23800.00 0.00 0.12340 23600.00 23800.00 5.00 0.12357 23600.00 23800.00 10.00 0.12372 23600.00 23800.00 15.00 0.12383 23600.00 23800.00 20.00 0.12392 23600.00 23800.00 25.00 0.12397 23600.00 23800.00 30.00 0.12398 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23568.33 23797.92 27.95 0.12401 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/451.ctf NX, NY, NZ: 512 512 1 MODE : real