CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/450.mrc Output diagnostic file name Micrographs_0601/450.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/450.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2402.000 8458.000 Mean, RMS : 4464.144 215.0553 TITLE 1: emi2mrc: 450.emi Mon Jan 06 14:58:49 2014 TITLE 2: kV 300, Mag 78000x, Def 19690, Spot 4, Int 55.06%, Obj 88.07%, Dif 73.67% TITLE 3: X 279.816, Y 848.897, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03690 10200.00 10000.00 0.00 0.03931 10400.00 10000.00 0.00 0.04173 10600.00 10000.00 0.00 0.04444 10800.00 10000.00 0.00 0.04723 11000.00 10000.00 0.00 0.05033 11200.00 10000.00 0.00 0.05360 11400.00 10000.00 0.00 0.05702 11600.00 10000.00 0.00 0.06063 11800.00 10000.00 0.00 0.06436 12000.00 10000.00 0.00 0.06840 12200.00 10000.00 0.00 0.07250 12400.00 10000.00 0.00 0.07693 12600.00 10000.00 0.00 0.08146 12800.00 10000.00 0.00 0.08635 13000.00 10000.00 0.00 0.09162 13200.00 10000.00 0.00 0.09661 13400.00 10000.00 0.00 0.10040 13600.00 10000.00 0.00 0.10178 13400.00 10200.00 0.00 0.10355 13600.00 10200.00 0.00 0.10475 13400.00 10400.00 0.00 0.10653 13600.00 10400.00 0.00 0.10761 13400.00 10600.00 0.00 0.10987 13600.00 10600.00 0.00 0.11090 13400.00 10800.00 0.00 0.11338 13600.00 10800.00 0.00 0.11430 13400.00 11000.00 0.00 0.11714 13600.00 11000.00 0.00 0.11789 13400.00 11200.00 0.00 0.12081 13600.00 11200.00 0.00 0.12140 13400.00 11400.00 0.00 0.12489 13600.00 11400.00 0.00 0.12530 13200.00 11600.00 0.00 0.12540 13400.00 11600.00 0.00 0.12882 13600.00 11600.00 0.00 0.12904 13200.00 11800.00 0.00 0.12951 13400.00 11800.00 0.00 0.13281 13600.00 11800.00 0.00 0.13284 13200.00 12000.00 0.00 0.13393 13400.00 12000.00 0.00 0.13715 13200.00 12200.00 0.00 0.13868 13400.00 12200.00 0.00 0.14176 13200.00 12400.00 0.00 0.14365 13400.00 12400.00 0.00 0.14649 13200.00 12600.00 0.00 0.14818 13400.00 12600.00 0.00 0.15077 13200.00 12800.00 0.00 0.15295 13400.00 12800.00 0.00 0.15506 13200.00 13000.00 0.00 0.15678 13400.00 13000.00 0.00 0.15831 13200.00 13200.00 0.00 0.16038 13400.00 13200.00 0.00 0.16101 13200.00 13400.00 0.00 0.16213 13200.00 13400.00 5.00 0.16222 13200.00 13400.00 10.00 0.16228 13200.00 13400.00 15.00 0.16233 13200.00 13400.00 20.00 0.16235 13200.00 13400.00 25.00 0.16236 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13271.87 13368.53 19.14 0.16264 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/450.ctf NX, NY, NZ: 512 512 1 MODE : real