CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/447.mrc Output diagnostic file name Micrographs_0601/447.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/447.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2362.000 7974.000 Mean, RMS : 4468.473 216.2296 TITLE 1: emi2mrc: 447.emi Mon Jan 06 14:55:50 2014 TITLE 2: kV 300, Mag 78000x, Def 19690, Spot 4, Int 55.06%, Obj 88.07%, Dif 73.67% TITLE 3: X 281.505, Y 851.460, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01125 10200.00 10000.00 0.00 0.01195 10400.00 10000.00 0.00 0.01270 10600.00 10000.00 0.00 0.01347 10800.00 10000.00 0.00 0.01422 11000.00 10000.00 0.00 0.01510 11200.00 10000.00 0.00 0.01618 11400.00 10000.00 0.00 0.01755 11600.00 10000.00 0.00 0.01929 11800.00 10000.00 0.00 0.02112 12000.00 10000.00 0.00 0.02302 12200.00 10000.00 0.00 0.02497 12400.00 10000.00 0.00 0.02707 12600.00 10000.00 0.00 0.02935 12800.00 10000.00 0.00 0.03180 13000.00 10000.00 0.00 0.03420 13200.00 10000.00 0.00 0.03655 13400.00 10000.00 0.00 0.03906 13600.00 10000.00 0.00 0.04151 13800.00 10000.00 0.00 0.04400 14000.00 10000.00 0.00 0.04684 14200.00 10000.00 0.00 0.04981 14400.00 10000.00 0.00 0.05277 14600.00 10000.00 0.00 0.05607 14800.00 10000.00 0.00 0.05960 15000.00 10000.00 0.00 0.06314 15200.00 10000.00 0.00 0.06690 15400.00 10000.00 0.00 0.07070 15600.00 10000.00 0.00 0.07365 15800.00 10000.00 0.00 0.07479 16000.00 10000.00 0.00 0.07495 15600.00 10200.00 0.00 0.07503 15800.00 10200.00 0.00 0.07623 16000.00 10200.00 0.00 0.07646 15600.00 10400.00 0.00 0.07668 15800.00 10400.00 0.00 0.07795 16000.00 10400.00 0.00 0.07819 15600.00 10600.00 0.00 0.07862 15800.00 10600.00 0.00 0.07992 16000.00 10600.00 0.00 0.08017 15600.00 10800.00 0.00 0.08081 15800.00 10800.00 0.00 0.08210 16000.00 10800.00 0.00 0.08230 15600.00 11000.00 0.00 0.08303 15800.00 11000.00 0.00 0.08429 16000.00 11000.00 0.00 0.08443 15600.00 11200.00 0.00 0.08517 15800.00 11200.00 0.00 0.08640 16000.00 11200.00 0.00 0.08646 15600.00 11400.00 0.00 0.08747 15800.00 11400.00 0.00 0.08862 15600.00 11600.00 0.00 0.08965 15800.00 11600.00 0.00 0.09076 15600.00 11800.00 0.00 0.09216 15800.00 11800.00 0.00 0.09328 15600.00 12000.00 0.00 0.09475 15800.00 12000.00 0.00 0.09589 15600.00 12200.00 0.00 0.09744 15800.00 12200.00 0.00 0.09860 15600.00 12400.00 0.00 0.10041 15800.00 12400.00 0.00 0.10156 15600.00 12600.00 0.00 0.10328 15800.00 12600.00 0.00 0.10435 15600.00 12800.00 0.00 0.10635 15800.00 12800.00 0.00 0.10734 15600.00 13000.00 0.00 0.10931 15800.00 13000.00 0.00 0.11024 15600.00 13200.00 0.00 0.11258 15800.00 13200.00 0.00 0.11344 15600.00 13400.00 0.00 0.11592 15800.00 13400.00 0.00 0.11668 15600.00 13600.00 0.00 0.11946 15800.00 13600.00 0.00 0.12007 15600.00 13800.00 0.00 0.12309 15800.00 13800.00 0.00 0.12351 15600.00 14000.00 0.00 0.12649 15800.00 14000.00 0.00 0.12675 15400.00 14200.00 0.00 0.12716 15600.00 14200.00 0.00 0.13046 15800.00 14200.00 0.00 0.13048 15400.00 14400.00 0.00 0.13108 15600.00 14400.00 0.00 0.13424 15400.00 14600.00 0.00 0.13524 15600.00 14600.00 0.00 0.13817 15400.00 14800.00 0.00 0.13946 15600.00 14800.00 0.00 0.14220 15400.00 15000.00 0.00 0.14411 15600.00 15000.00 0.00 0.14661 15400.00 15200.00 0.00 0.14867 15600.00 15200.00 0.00 0.15076 15400.00 15400.00 0.00 0.15245 15600.00 15400.00 0.00 0.15397 15400.00 15600.00 0.00 0.15573 15600.00 15600.00 0.00 0.15591 15400.00 15600.00 10.00 0.15595 15400.00 15600.00 15.00 0.15601 15400.00 15600.00 20.00 0.15602 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15481.54 15647.03 12.63 0.15672 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/447.ctf NX, NY, NZ: 512 512 1 MODE : real