CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/445.mrc Output diagnostic file name Micrographs_0601/445.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/445.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2512.000 8200.000 Mean, RMS : 4491.737 222.2347 TITLE 1: emi2mrc: 445.emi Mon Jan 06 14:53:51 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 285.213, Y 847.401, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01675 10400.00 10000.00 0.00 -0.01661 10600.00 10000.00 0.00 -0.01655 10800.00 10000.00 0.00 -0.01652 11000.00 10000.00 0.00 -0.01649 11200.00 10000.00 0.00 -0.01646 11400.00 10000.00 0.00 -0.01634 12000.00 10000.00 0.00 -0.01614 12200.00 10000.00 0.00 -0.01583 12400.00 10000.00 0.00 -0.01554 12600.00 10000.00 0.00 -0.01517 12800.00 10000.00 0.00 -0.01497 13000.00 10000.00 0.00 -0.01475 13200.00 10000.00 0.00 -0.01438 13400.00 10000.00 0.00 -0.01407 13600.00 10000.00 0.00 -0.01383 13800.00 10000.00 0.00 -0.01362 14000.00 10000.00 0.00 -0.01339 14200.00 10000.00 0.00 -0.01301 14400.00 10000.00 0.00 -0.01252 14600.00 10000.00 0.00 -0.01195 14800.00 10000.00 0.00 -0.01133 15000.00 10000.00 0.00 -0.01072 15200.00 10000.00 0.00 -0.01007 15400.00 10000.00 0.00 -0.00934 15600.00 10000.00 0.00 -0.00856 15800.00 10000.00 0.00 -0.00778 16000.00 10000.00 0.00 -0.00703 16200.00 10000.00 0.00 -0.00614 16400.00 10000.00 0.00 -0.00541 16600.00 10000.00 0.00 -0.00477 16800.00 10000.00 0.00 -0.00378 17000.00 10000.00 0.00 -0.00271 17200.00 10000.00 0.00 -0.00168 17400.00 10000.00 0.00 -0.00062 17600.00 10000.00 0.00 0.00041 17800.00 10000.00 0.00 0.00143 18000.00 10000.00 0.00 0.00257 18200.00 10000.00 0.00 0.00375 18400.00 10000.00 0.00 0.00483 18600.00 10000.00 0.00 0.00603 18800.00 10000.00 0.00 0.00724 19000.00 10000.00 0.00 0.00831 19200.00 10000.00 0.00 0.00951 19400.00 10000.00 0.00 0.01082 19600.00 10000.00 0.00 0.01218 19800.00 10000.00 0.00 0.01342 20000.00 10000.00 0.00 0.01443 20200.00 10000.00 0.00 0.01559 20400.00 10000.00 0.00 0.01702 20600.00 10000.00 0.00 0.01850 20800.00 10000.00 0.00 0.02008 21000.00 10000.00 0.00 0.02178 21200.00 10000.00 0.00 0.02359 21400.00 10000.00 0.00 0.02557 21600.00 10000.00 0.00 0.02756 21800.00 10000.00 0.00 0.02970 22000.00 10000.00 0.00 0.03199 22200.00 10000.00 0.00 0.03385 22400.00 10000.00 0.00 0.03511 22600.00 10000.00 0.00 0.03593 22800.00 10000.00 0.00 0.03647 23000.00 10000.00 0.00 0.03675 23200.00 10000.00 0.00 0.03688 23400.00 10000.00 0.00 0.03698 22800.00 10200.00 0.00 0.03714 23000.00 10200.00 0.00 0.03741 23200.00 10200.00 0.00 0.03755 23400.00 10200.00 0.00 0.03762 22800.00 10400.00 0.00 0.03774 23000.00 10400.00 0.00 0.03801 23200.00 10400.00 0.00 0.03816 23400.00 10400.00 0.00 0.03820 22800.00 10600.00 0.00 0.03844 23000.00 10600.00 0.00 0.03875 23200.00 10600.00 0.00 0.03891 23400.00 10600.00 0.00 0.03891 22800.00 10800.00 0.00 0.03921 23000.00 10800.00 0.00 0.03955 23200.00 10800.00 0.00 0.03968 22800.00 11000.00 0.00 0.03995 23000.00 11000.00 0.00 0.04031 23200.00 11000.00 0.00 0.04038 22800.00 11200.00 0.00 0.04067 23000.00 11200.00 0.00 0.04101 23200.00 11200.00 0.00 0.04101 22800.00 11400.00 0.00 0.04137 23000.00 11400.00 0.00 0.04164 22800.00 11600.00 0.00 0.04186 23000.00 11600.00 0.00 0.04204 22800.00 11800.00 0.00 0.04232 23000.00 11800.00 0.00 0.04245 22600.00 12000.00 0.00 0.04257 22800.00 12000.00 0.00 0.04301 23000.00 12000.00 0.00 0.04312 22600.00 12200.00 0.00 0.04351 22800.00 12200.00 0.00 0.04390 23000.00 12200.00 0.00 0.04399 22600.00 12400.00 0.00 0.04443 22800.00 12400.00 0.00 0.04476 23000.00 12400.00 0.00 0.04485 22600.00 12600.00 0.00 0.04518 22800.00 12600.00 0.00 0.04552 23000.00 12600.00 0.00 0.04564 22600.00 12800.00 0.00 0.04603 22800.00 12800.00 0.00 0.04637 23000.00 12800.00 0.00 0.04650 22600.00 13000.00 0.00 0.04681 22800.00 13000.00 0.00 0.04714 23000.00 13000.00 0.00 0.04728 23200.00 13000.00 0.00 0.04729 22600.00 13200.00 0.00 0.04760 22800.00 13200.00 0.00 0.04796 23000.00 13200.00 0.00 0.04815 23200.00 13200.00 0.00 0.04818 22600.00 13400.00 0.00 0.04848 22800.00 13400.00 0.00 0.04885 23000.00 13400.00 0.00 0.04906 23200.00 13400.00 0.00 0.04907 22600.00 13600.00 0.00 0.04935 22800.00 13600.00 0.00 0.04973 23000.00 13600.00 0.00 0.04993 22600.00 13800.00 0.00 0.05026 22800.00 13800.00 0.00 0.05064 23000.00 13800.00 0.00 0.05085 22600.00 14000.00 0.00 0.05117 22800.00 14000.00 0.00 0.05159 23000.00 14000.00 0.00 0.05184 22600.00 14200.00 0.00 0.05221 22800.00 14200.00 0.00 0.05266 23000.00 14200.00 0.00 0.05291 22600.00 14400.00 0.00 0.05323 22800.00 14400.00 0.00 0.05366 23000.00 14400.00 0.00 0.05385 22600.00 14600.00 0.00 0.05430 22800.00 14600.00 0.00 0.05468 23000.00 14600.00 0.00 0.05486 22600.00 14800.00 0.00 0.05544 22800.00 14800.00 0.00 0.05586 23000.00 14800.00 0.00 0.05606 22600.00 15000.00 0.00 0.05673 22800.00 15000.00 0.00 0.05719 23000.00 15000.00 0.00 0.05737 22600.00 15200.00 0.00 0.05816 22800.00 15200.00 0.00 0.05861 23000.00 15200.00 0.00 0.05867 22400.00 15400.00 0.00 0.05875 22600.00 15400.00 0.00 0.05956 22800.00 15400.00 0.00 0.05992 22400.00 15600.00 0.00 0.06004 22600.00 15600.00 0.00 0.06080 22800.00 15600.00 0.00 0.06107 22400.00 15800.00 0.00 0.06122 22600.00 15800.00 0.00 0.06192 22800.00 15800.00 0.00 0.06213 22400.00 16000.00 0.00 0.06260 22600.00 16000.00 0.00 0.06328 22800.00 16000.00 0.00 0.06349 22400.00 16200.00 0.00 0.06406 22600.00 16200.00 0.00 0.06473 22800.00 16200.00 0.00 0.06494 22400.00 16400.00 0.00 0.06543 22600.00 16400.00 0.00 0.06611 22800.00 16400.00 0.00 0.06629 22400.00 16600.00 0.00 0.06687 22600.00 16600.00 0.00 0.06756 22800.00 16600.00 0.00 0.06771 22400.00 16800.00 0.00 0.06854 22600.00 16800.00 0.00 0.06919 22800.00 16800.00 0.00 0.06930 22400.00 17000.00 0.00 0.07012 22600.00 17000.00 0.00 0.07071 22800.00 17000.00 0.00 0.07078 22400.00 17200.00 0.00 0.07169 22600.00 17200.00 0.00 0.07225 22800.00 17200.00 0.00 0.07229 22400.00 17400.00 0.00 0.07350 22600.00 17400.00 0.00 0.07402 22400.00 17600.00 0.00 0.07513 22600.00 17600.00 0.00 0.07560 22400.00 17800.00 0.00 0.07688 22600.00 17800.00 0.00 0.07727 22400.00 18000.00 0.00 0.07871 22600.00 18000.00 0.00 0.07905 22200.00 18200.00 0.00 0.07918 22400.00 18200.00 0.00 0.08065 22600.00 18200.00 0.00 0.08091 22200.00 18400.00 0.00 0.08125 22400.00 18400.00 0.00 0.08263 22600.00 18400.00 0.00 0.08276 22200.00 18600.00 0.00 0.08303 22400.00 18600.00 0.00 0.08429 22600.00 18600.00 0.00 0.08429 22200.00 18800.00 0.00 0.08477 22400.00 18800.00 0.00 0.08595 22200.00 19000.00 0.00 0.08660 22400.00 19000.00 0.00 0.08775 22200.00 19200.00 0.00 0.08858 22400.00 19200.00 0.00 0.08972 22200.00 19400.00 0.00 0.09068 22400.00 19400.00 0.00 0.09180 22200.00 19600.00 0.00 0.09302 22400.00 19600.00 0.00 0.09407 22200.00 19800.00 0.00 0.09539 22400.00 19800.00 0.00 0.09634 22200.00 20000.00 0.00 0.09777 22400.00 20000.00 0.00 0.09862 22200.00 20200.00 0.00 0.10029 22400.00 20200.00 0.00 0.10106 22200.00 20400.00 0.00 0.10285 22400.00 20400.00 0.00 0.10351 22200.00 20600.00 0.00 0.10566 22400.00 20600.00 0.00 0.10616 22200.00 20800.00 0.00 0.10849 22400.00 20800.00 0.00 0.10879 22000.00 21000.00 0.00 0.10912 22200.00 21000.00 0.00 0.11141 22400.00 21000.00 0.00 0.11149 22000.00 21200.00 0.00 0.11222 22200.00 21200.00 0.00 0.11431 22000.00 21400.00 0.00 0.11536 22200.00 21400.00 0.00 0.11727 22000.00 21600.00 0.00 0.11843 22200.00 21600.00 0.00 0.12016 22000.00 21800.00 0.00 0.12154 22200.00 21800.00 0.00 0.12305 22000.00 22000.00 0.00 0.12498 22200.00 22000.00 0.00 0.12582 22000.00 22200.00 0.00 0.12672 22000.00 22200.00 5.00 0.12688 22000.00 22200.00 10.00 0.12704 22000.00 22200.00 15.00 0.12717 22000.00 22200.00 20.00 0.12727 22000.00 22200.00 25.00 0.12735 22000.00 22200.00 30.00 0.12740 22000.00 22200.00 35.00 0.12741 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22035.71 22264.50 32.72 0.12770 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/445.ctf NX, NY, NZ: 512 512 1 MODE : real