CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/444.mrc Output diagnostic file name Micrographs_0601/444.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/444.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2224.000 11770.00 Mean, RMS : 4494.553 219.6200 TITLE 1: emi2mrc: 444.emi Mon Jan 06 14:53:05 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 287.085, Y 845.637, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01085 10200.00 10000.00 0.00 -0.01070 10400.00 10000.00 0.00 -0.01045 10600.00 10000.00 0.00 -0.01017 10800.00 10000.00 0.00 -0.00992 11000.00 10000.00 0.00 -0.00977 11200.00 10000.00 0.00 -0.00951 11400.00 10000.00 0.00 -0.00925 11600.00 10000.00 0.00 -0.00891 11800.00 10000.00 0.00 -0.00860 12000.00 10000.00 0.00 -0.00844 12200.00 10000.00 0.00 -0.00821 12400.00 10000.00 0.00 -0.00781 12600.00 10000.00 0.00 -0.00728 12800.00 10000.00 0.00 -0.00677 13000.00 10000.00 0.00 -0.00625 13200.00 10000.00 0.00 -0.00571 13400.00 10000.00 0.00 -0.00521 13600.00 10000.00 0.00 -0.00466 13800.00 10000.00 0.00 -0.00412 14000.00 10000.00 0.00 -0.00362 14200.00 10000.00 0.00 -0.00311 14400.00 10000.00 0.00 -0.00242 14600.00 10000.00 0.00 -0.00164 14800.00 10000.00 0.00 -0.00098 15000.00 10000.00 0.00 -0.00029 15200.00 10000.00 0.00 0.00045 15400.00 10000.00 0.00 0.00111 15600.00 10000.00 0.00 0.00174 15800.00 10000.00 0.00 0.00246 16000.00 10000.00 0.00 0.00328 16200.00 10000.00 0.00 0.00412 16400.00 10000.00 0.00 0.00512 16600.00 10000.00 0.00 0.00621 16800.00 10000.00 0.00 0.00732 17000.00 10000.00 0.00 0.00854 17200.00 10000.00 0.00 0.00977 17400.00 10000.00 0.00 0.01104 17600.00 10000.00 0.00 0.01224 17800.00 10000.00 0.00 0.01340 18000.00 10000.00 0.00 0.01470 18200.00 10000.00 0.00 0.01599 18400.00 10000.00 0.00 0.01726 18600.00 10000.00 0.00 0.01853 18800.00 10000.00 0.00 0.02000 19000.00 10000.00 0.00 0.02157 19200.00 10000.00 0.00 0.02306 19400.00 10000.00 0.00 0.02472 19600.00 10000.00 0.00 0.02635 19800.00 10000.00 0.00 0.02799 20000.00 10000.00 0.00 0.02982 20200.00 10000.00 0.00 0.03204 20400.00 10000.00 0.00 0.03458 20600.00 10000.00 0.00 0.03708 20800.00 10000.00 0.00 0.03962 21000.00 10000.00 0.00 0.04204 21200.00 10000.00 0.00 0.04381 21400.00 10000.00 0.00 0.04484 21600.00 10000.00 0.00 0.04523 21400.00 10200.00 0.00 0.04549 21600.00 10200.00 0.00 0.04579 21400.00 10400.00 0.00 0.04598 21600.00 10400.00 0.00 0.04624 22400.00 10400.00 0.00 0.04626 21400.00 10600.00 0.00 0.04663 21600.00 10600.00 0.00 0.04688 22200.00 10600.00 0.00 0.04698 22400.00 10600.00 0.00 0.04698 21400.00 10800.00 0.00 0.04733 21600.00 10800.00 0.00 0.04755 22000.00 10800.00 0.00 0.04756 22200.00 10800.00 0.00 0.04766 21400.00 11000.00 0.00 0.04803 21600.00 11000.00 0.00 0.04823 21800.00 11000.00 0.00 0.04826 22000.00 11000.00 0.00 0.04834 22200.00 11000.00 0.00 0.04843 21400.00 11200.00 0.00 0.04856 21600.00 11200.00 0.00 0.04879 21800.00 11200.00 0.00 0.04888 22000.00 11200.00 0.00 0.04897 22200.00 11200.00 0.00 0.04901 21400.00 11400.00 0.00 0.04919 21600.00 11400.00 0.00 0.04946 21800.00 11400.00 0.00 0.04958 22000.00 11400.00 0.00 0.04963 22200.00 11400.00 0.00 0.04963 21400.00 11600.00 0.00 0.05002 21600.00 11600.00 0.00 0.05029 21800.00 11600.00 0.00 0.05041 22000.00 11600.00 0.00 0.05043 21400.00 11800.00 0.00 0.05093 21600.00 11800.00 0.00 0.05117 21800.00 11800.00 0.00 0.05126 21400.00 12000.00 0.00 0.05182 21600.00 12000.00 0.00 0.05201 21800.00 12000.00 0.00 0.05207 21400.00 12200.00 0.00 0.05259 21600.00 12200.00 0.00 0.05276 21800.00 12200.00 0.00 0.05283 21400.00 12400.00 0.00 0.05339 21600.00 12400.00 0.00 0.05358 21800.00 12400.00 0.00 0.05367 22200.00 12400.00 0.00 0.05371 21400.00 12600.00 0.00 0.05429 21600.00 12600.00 0.00 0.05452 21800.00 12600.00 0.00 0.05463 22000.00 12600.00 0.00 0.05465 22200.00 12600.00 0.00 0.05468 21400.00 12800.00 0.00 0.05528 21600.00 12800.00 0.00 0.05553 21800.00 12800.00 0.00 0.05565 22000.00 12800.00 0.00 0.05568 21400.00 13000.00 0.00 0.05628 21600.00 13000.00 0.00 0.05656 21800.00 13000.00 0.00 0.05670 22000.00 13000.00 0.00 0.05673 21400.00 13200.00 0.00 0.05739 21600.00 13200.00 0.00 0.05773 21800.00 13200.00 0.00 0.05789 21400.00 13400.00 0.00 0.05858 21600.00 13400.00 0.00 0.05893 21800.00 13400.00 0.00 0.05906 21200.00 13600.00 0.00 0.05907 21400.00 13600.00 0.00 0.05979 21600.00 13600.00 0.00 0.06014 21800.00 13600.00 0.00 0.06025 21200.00 13800.00 0.00 0.06025 21400.00 13800.00 0.00 0.06101 21600.00 13800.00 0.00 0.06140 21800.00 13800.00 0.00 0.06151 21400.00 14000.00 0.00 0.06226 21600.00 14000.00 0.00 0.06267 21800.00 14000.00 0.00 0.06272 21400.00 14200.00 0.00 0.06341 21600.00 14200.00 0.00 0.06376 21400.00 14400.00 0.00 0.06452 21600.00 14400.00 0.00 0.06480 21200.00 14600.00 0.00 0.06503 21400.00 14600.00 0.00 0.06580 21600.00 14600.00 0.00 0.06605 21200.00 14800.00 0.00 0.06630 21400.00 14800.00 0.00 0.06706 21600.00 14800.00 0.00 0.06731 21200.00 15000.00 0.00 0.06786 21400.00 15000.00 0.00 0.06860 21600.00 15000.00 0.00 0.06883 21200.00 15200.00 0.00 0.06951 21400.00 15200.00 0.00 0.07021 21600.00 15200.00 0.00 0.07043 21200.00 15400.00 0.00 0.07105 21400.00 15400.00 0.00 0.07174 21600.00 15400.00 0.00 0.07194 21200.00 15600.00 0.00 0.07258 21400.00 15600.00 0.00 0.07328 21600.00 15600.00 0.00 0.07347 21200.00 15800.00 0.00 0.07412 21400.00 15800.00 0.00 0.07482 21600.00 15800.00 0.00 0.07501 21200.00 16000.00 0.00 0.07585 21400.00 16000.00 0.00 0.07656 21600.00 16000.00 0.00 0.07675 21200.00 16200.00 0.00 0.07756 21400.00 16200.00 0.00 0.07830 21600.00 16200.00 0.00 0.07849 21200.00 16400.00 0.00 0.07944 21400.00 16400.00 0.00 0.08020 21600.00 16400.00 0.00 0.08036 21200.00 16600.00 0.00 0.08140 21400.00 16600.00 0.00 0.08213 21600.00 16600.00 0.00 0.08220 21200.00 16800.00 0.00 0.08346 21400.00 16800.00 0.00 0.08409 21200.00 17000.00 0.00 0.08533 21400.00 17000.00 0.00 0.08590 21200.00 17200.00 0.00 0.08706 21400.00 17200.00 0.00 0.08762 21200.00 17400.00 0.00 0.08904 21400.00 17400.00 0.00 0.08957 21200.00 17600.00 0.00 0.09087 21400.00 17600.00 0.00 0.09135 21200.00 17800.00 0.00 0.09287 21400.00 17800.00 0.00 0.09333 21200.00 18000.00 0.00 0.09485 21400.00 18000.00 0.00 0.09527 21200.00 18200.00 0.00 0.09689 21400.00 18200.00 0.00 0.09727 21200.00 18400.00 0.00 0.09909 21400.00 18400.00 0.00 0.09940 21000.00 18600.00 0.00 0.09951 21200.00 18600.00 0.00 0.10139 21400.00 18600.00 0.00 0.10162 21000.00 18800.00 0.00 0.10222 21200.00 18800.00 0.00 0.10404 21400.00 18800.00 0.00 0.10416 21000.00 19000.00 0.00 0.10487 21200.00 19000.00 0.00 0.10664 21000.00 19200.00 0.00 0.10775 21200.00 19200.00 0.00 0.10943 21000.00 19400.00 0.00 0.11058 21200.00 19400.00 0.00 0.11213 21000.00 19600.00 0.00 0.11338 21200.00 19600.00 0.00 0.11483 21000.00 19800.00 0.00 0.11628 21200.00 19800.00 0.00 0.11761 21000.00 20000.00 0.00 0.11933 21200.00 20000.00 0.00 0.12049 21000.00 20200.00 0.00 0.12258 21200.00 20200.00 0.00 0.12347 21000.00 20400.00 0.00 0.12566 21200.00 20400.00 0.00 0.12620 21000.00 20600.00 0.00 0.12873 21200.00 20600.00 0.00 0.12881 20800.00 20800.00 0.00 0.12890 21000.00 20800.00 0.00 0.13130 21000.00 21000.00 0.00 0.13310 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20946.25 21094.12 35.28 0.13346 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/444.ctf NX, NY, NZ: 512 512 1 MODE : real