CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/443.mrc Output diagnostic file name Micrographs_0601/443.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/443.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2226.000 7336.000 Mean, RMS : 4499.531 217.1110 TITLE 1: emi2mrc: 443.emi Mon Jan 06 14:51:11 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 290.622, Y 841.197, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00781 10200.00 10000.00 0.00 0.00886 10400.00 10000.00 0.00 0.00994 10600.00 10000.00 0.00 0.01093 10800.00 10000.00 0.00 0.01182 11000.00 10000.00 0.00 0.01288 11200.00 10000.00 0.00 0.01385 11400.00 10000.00 0.00 0.01487 11600.00 10000.00 0.00 0.01608 11800.00 10000.00 0.00 0.01724 12000.00 10000.00 0.00 0.01864 12200.00 10000.00 0.00 0.02029 12400.00 10000.00 0.00 0.02222 12600.00 10000.00 0.00 0.02433 12800.00 10000.00 0.00 0.02652 13000.00 10000.00 0.00 0.02874 13200.00 10000.00 0.00 0.03101 13400.00 10000.00 0.00 0.03346 13600.00 10000.00 0.00 0.03588 13800.00 10000.00 0.00 0.03827 14000.00 10000.00 0.00 0.04069 14200.00 10000.00 0.00 0.04319 14400.00 10000.00 0.00 0.04581 14600.00 10000.00 0.00 0.04853 14800.00 10000.00 0.00 0.05139 15000.00 10000.00 0.00 0.05466 15200.00 10000.00 0.00 0.05836 15400.00 10000.00 0.00 0.06221 15600.00 10000.00 0.00 0.06625 15800.00 10000.00 0.00 0.06973 16000.00 10000.00 0.00 0.07178 16200.00 10000.00 0.00 0.07231 16000.00 10200.00 0.00 0.07343 16200.00 10200.00 0.00 0.07394 16000.00 10400.00 0.00 0.07511 16200.00 10400.00 0.00 0.07561 16000.00 10600.00 0.00 0.07678 16200.00 10600.00 0.00 0.07725 16000.00 10800.00 0.00 0.07837 16200.00 10800.00 0.00 0.07882 16000.00 11000.00 0.00 0.08018 16200.00 11000.00 0.00 0.08063 16000.00 11200.00 0.00 0.08211 16200.00 11200.00 0.00 0.08256 16000.00 11400.00 0.00 0.08429 16200.00 11400.00 0.00 0.08470 16000.00 11600.00 0.00 0.08647 16200.00 11600.00 0.00 0.08685 16000.00 11800.00 0.00 0.08903 16200.00 11800.00 0.00 0.08934 15800.00 12000.00 0.00 0.08948 16000.00 12000.00 0.00 0.09163 16200.00 12000.00 0.00 0.09191 15800.00 12200.00 0.00 0.09214 16000.00 12200.00 0.00 0.09427 16200.00 12200.00 0.00 0.09455 15800.00 12400.00 0.00 0.09519 16000.00 12400.00 0.00 0.09729 16200.00 12400.00 0.00 0.09752 15800.00 12600.00 0.00 0.09810 16000.00 12600.00 0.00 0.10015 16200.00 12600.00 0.00 0.10029 15800.00 12800.00 0.00 0.10108 16000.00 12800.00 0.00 0.10310 16200.00 12800.00 0.00 0.10316 15800.00 13000.00 0.00 0.10390 16000.00 13000.00 0.00 0.10594 16200.00 13000.00 0.00 0.10595 15800.00 13200.00 0.00 0.10692 16000.00 13200.00 0.00 0.10897 15800.00 13400.00 0.00 0.11019 16000.00 13400.00 0.00 0.11219 15800.00 13600.00 0.00 0.11364 16000.00 13600.00 0.00 0.11553 15800.00 13800.00 0.00 0.11727 16000.00 13800.00 0.00 0.11906 15800.00 14000.00 0.00 0.12103 16000.00 14000.00 0.00 0.12271 15800.00 14200.00 0.00 0.12502 16000.00 14200.00 0.00 0.12653 15800.00 14400.00 0.00 0.12868 16000.00 14400.00 0.00 0.12997 15800.00 14600.00 0.00 0.13253 16000.00 14600.00 0.00 0.13357 15800.00 14800.00 0.00 0.13640 16000.00 14800.00 0.00 0.13720 15800.00 15000.00 0.00 0.14086 16000.00 15000.00 0.00 0.14131 15600.00 15200.00 0.00 0.14177 15800.00 15200.00 0.00 0.14549 15600.00 15400.00 0.00 0.14648 15800.00 15400.00 0.00 0.14985 15600.00 15600.00 0.00 0.15186 15800.00 15600.00 0.00 0.15434 15600.00 15800.00 0.00 0.15549 15800.00 15800.00 0.00 0.15665 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15709.84 15926.23 15.11 0.15736 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/443.ctf NX, NY, NZ: 512 512 1 MODE : real