CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/442.mrc Output diagnostic file name Micrographs_0601/442.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/442.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2524.000 8802.000 Mean, RMS : 4459.351 216.2240 TITLE 1: emi2mrc: 442.emi Mon Jan 06 14:44:49 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.06%, Obj 88.07%, Dif 73.67% TITLE 3: X 283.567, Y 852.827, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01591 10200.00 10000.00 0.00 0.01715 10400.00 10000.00 0.00 0.01828 10600.00 10000.00 0.00 0.01977 10800.00 10000.00 0.00 0.02121 11000.00 10000.00 0.00 0.02278 11200.00 10000.00 0.00 0.02455 11400.00 10000.00 0.00 0.02635 11600.00 10000.00 0.00 0.02834 11800.00 10000.00 0.00 0.03028 12000.00 10000.00 0.00 0.03244 12200.00 10000.00 0.00 0.03465 12400.00 10000.00 0.00 0.03701 12600.00 10000.00 0.00 0.03952 12800.00 10000.00 0.00 0.04198 13000.00 10000.00 0.00 0.04476 13200.00 10000.00 0.00 0.04754 13400.00 10000.00 0.00 0.05030 13600.00 10000.00 0.00 0.05334 13800.00 10000.00 0.00 0.05661 14000.00 10000.00 0.00 0.05992 14200.00 10000.00 0.00 0.06352 14400.00 10000.00 0.00 0.06749 14600.00 10000.00 0.00 0.07161 14800.00 10000.00 0.00 0.07580 15000.00 10000.00 0.00 0.07921 15200.00 10000.00 0.00 0.08107 15400.00 10000.00 0.00 0.08142 15200.00 10200.00 0.00 0.08290 15400.00 10200.00 0.00 0.08322 15200.00 10400.00 0.00 0.08495 15400.00 10400.00 0.00 0.08525 15000.00 10600.00 0.00 0.08534 15200.00 10600.00 0.00 0.08731 15400.00 10600.00 0.00 0.08761 15000.00 10800.00 0.00 0.08766 15200.00 10800.00 0.00 0.08964 15400.00 10800.00 0.00 0.08987 15000.00 11000.00 0.00 0.09005 15200.00 11000.00 0.00 0.09201 15400.00 11000.00 0.00 0.09213 15000.00 11200.00 0.00 0.09245 15200.00 11200.00 0.00 0.09443 15400.00 11200.00 0.00 0.09449 15000.00 11400.00 0.00 0.09536 15200.00 11400.00 0.00 0.09732 15000.00 11600.00 0.00 0.09844 15200.00 11600.00 0.00 0.10031 15000.00 11800.00 0.00 0.10169 15200.00 11800.00 0.00 0.10341 15000.00 12000.00 0.00 0.10482 15200.00 12000.00 0.00 0.10641 15000.00 12200.00 0.00 0.10797 15200.00 12200.00 0.00 0.10950 15000.00 12400.00 0.00 0.11149 15200.00 12400.00 0.00 0.11297 15000.00 12600.00 0.00 0.11486 15200.00 12600.00 0.00 0.11622 15000.00 12800.00 0.00 0.11843 15200.00 12800.00 0.00 0.11967 15000.00 13000.00 0.00 0.12187 15200.00 13000.00 0.00 0.12303 15000.00 13200.00 0.00 0.12545 15200.00 13200.00 0.00 0.12660 15000.00 13400.00 0.00 0.12926 15200.00 13400.00 0.00 0.13035 15000.00 13600.00 0.00 0.13330 15200.00 13600.00 0.00 0.13411 15000.00 13800.00 0.00 0.13719 15200.00 13800.00 0.00 0.13765 15000.00 14000.00 0.00 0.14082 15200.00 14000.00 0.00 0.14103 14800.00 14200.00 0.00 0.14204 15000.00 14200.00 0.00 0.14502 14800.00 14400.00 0.00 0.14604 15000.00 14400.00 0.00 0.14875 14800.00 14600.00 0.00 0.15027 15000.00 14600.00 0.00 0.15250 14800.00 14800.00 0.00 0.15435 15000.00 14800.00 0.00 0.15591 14800.00 15000.00 0.00 0.15743 15000.00 15000.00 0.00 0.15841 14800.00 15200.00 0.00 0.15848 14800.00 15200.00 5.00 0.15857 14800.00 15200.00 10.00 0.15859 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14916.04 15126.64 8.51 0.15916 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/442.ctf NX, NY, NZ: 512 512 1 MODE : real