CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/441.mrc Output diagnostic file name Micrographs_0601/441.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/441.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2436.000 8026.000 Mean, RMS : 4467.208 216.7573 TITLE 1: emi2mrc: 441.emi Mon Jan 06 14:48:56 2014 TITLE 2: kV 300, Mag 78000x, Def 19690, Spot 4, Int 55.06%, Obj 88.07%, Dif 73.67% TITLE 3: X 290.951, Y 844.819, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04741 10200.00 10000.00 0.00 0.05059 10400.00 10000.00 0.00 0.05356 10600.00 10000.00 0.00 0.05713 10800.00 10000.00 0.00 0.06087 11000.00 10000.00 0.00 0.06494 11200.00 10000.00 0.00 0.06900 11400.00 10000.00 0.00 0.07320 11600.00 10000.00 0.00 0.07778 11800.00 10000.00 0.00 0.08221 12000.00 10000.00 0.00 0.08699 12200.00 10000.00 0.00 0.09189 12400.00 10000.00 0.00 0.09736 12600.00 10000.00 0.00 0.10277 12800.00 10000.00 0.00 0.10650 13000.00 10000.00 0.00 0.10788 12800.00 10200.00 0.00 0.10990 13000.00 10200.00 0.00 0.11118 12800.00 10400.00 0.00 0.11353 13000.00 10400.00 0.00 0.11477 12800.00 10600.00 0.00 0.11743 13000.00 10600.00 0.00 0.11860 12800.00 10800.00 0.00 0.12144 13000.00 10800.00 0.00 0.12252 12800.00 11000.00 0.00 0.12601 13000.00 11000.00 0.00 0.12697 12800.00 11200.00 0.00 0.13049 13000.00 11200.00 0.00 0.13132 12600.00 11400.00 0.00 0.13163 12800.00 11400.00 0.00 0.13516 13000.00 11400.00 0.00 0.13583 12600.00 11600.00 0.00 0.13653 12800.00 11600.00 0.00 0.14001 13000.00 11600.00 0.00 0.14044 12600.00 11800.00 0.00 0.14191 12800.00 11800.00 0.00 0.14521 13000.00 11800.00 0.00 0.14526 12600.00 12000.00 0.00 0.14717 12800.00 12000.00 0.00 0.15021 12600.00 12200.00 0.00 0.15239 12800.00 12200.00 0.00 0.15505 12600.00 12400.00 0.00 0.15789 12800.00 12400.00 0.00 0.15991 12600.00 12600.00 0.00 0.16196 12800.00 12600.00 0.00 0.16341 12600.00 12800.00 0.00 0.16506 12800.00 12800.00 0.00 0.16541 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12697.76 12844.43 14.37 0.16599 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/441.ctf NX, NY, NZ: 512 512 1 MODE : real