CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/440.mrc Output diagnostic file name Micrographs_0601/440.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/440.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2266.000 8040.000 Mean, RMS : 4470.278 214.9015 TITLE 1: emi2mrc: 440.emi Mon Jan 06 14:47:59 2014 TITLE 2: kV 300, Mag 78000x, Def 19690, Spot 4, Int 55.06%, Obj 88.07%, Dif 73.67% TITLE 3: X 288.935, Y 846.545, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02547 10200.00 10000.00 0.00 0.02735 10400.00 10000.00 0.00 0.02912 10600.00 10000.00 0.00 0.03113 10800.00 10000.00 0.00 0.03306 11000.00 10000.00 0.00 0.03498 11200.00 10000.00 0.00 0.03722 11400.00 10000.00 0.00 0.03981 11600.00 10000.00 0.00 0.04262 11800.00 10000.00 0.00 0.04567 12000.00 10000.00 0.00 0.04894 12200.00 10000.00 0.00 0.05201 12400.00 10000.00 0.00 0.05518 12600.00 10000.00 0.00 0.05847 12800.00 10000.00 0.00 0.06197 13000.00 10000.00 0.00 0.06565 13200.00 10000.00 0.00 0.06956 13400.00 10000.00 0.00 0.07397 13600.00 10000.00 0.00 0.07835 13800.00 10000.00 0.00 0.08288 14000.00 10000.00 0.00 0.08724 14200.00 10000.00 0.00 0.09042 14400.00 10000.00 0.00 0.09168 14200.00 10200.00 0.00 0.09244 14400.00 10200.00 0.00 0.09363 14200.00 10400.00 0.00 0.09454 14400.00 10400.00 0.00 0.09563 14200.00 10600.00 0.00 0.09687 14400.00 10600.00 0.00 0.09791 14200.00 10800.00 0.00 0.09941 14400.00 10800.00 0.00 0.10043 14200.00 11000.00 0.00 0.10235 14400.00 11000.00 0.00 0.10334 14200.00 11200.00 0.00 0.10510 14400.00 11200.00 0.00 0.10607 14200.00 11400.00 0.00 0.10808 14400.00 11400.00 0.00 0.10907 14200.00 11600.00 0.00 0.11134 14400.00 11600.00 0.00 0.11230 14200.00 11800.00 0.00 0.11500 14400.00 11800.00 0.00 0.11584 14200.00 12000.00 0.00 0.11869 14400.00 12000.00 0.00 0.11941 14200.00 12200.00 0.00 0.12258 14400.00 12200.00 0.00 0.12317 14000.00 12400.00 0.00 0.12365 14200.00 12400.00 0.00 0.12678 14400.00 12400.00 0.00 0.12716 14000.00 12600.00 0.00 0.12765 14200.00 12600.00 0.00 0.13068 14400.00 12600.00 0.00 0.13082 14000.00 12800.00 0.00 0.13199 14200.00 12800.00 0.00 0.13489 14000.00 13000.00 0.00 0.13613 14200.00 13000.00 0.00 0.13890 14000.00 13200.00 0.00 0.14074 14200.00 13200.00 0.00 0.14327 14000.00 13400.00 0.00 0.14534 14200.00 13400.00 0.00 0.14752 14000.00 13600.00 0.00 0.14969 14200.00 13600.00 0.00 0.15146 14000.00 13800.00 0.00 0.15380 14200.00 13800.00 0.00 0.15503 14000.00 14000.00 0.00 0.15675 14200.00 14000.00 0.00 0.15745 14000.00 14200.00 0.00 0.15915 14000.00 14200.00 5.00 0.15925 14000.00 14200.00 10.00 0.15932 14000.00 14200.00 15.00 0.15936 14000.00 14200.00 20.00 0.15936 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14000.00 14200.00 17.58 0.15936 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/440.ctf NX, NY, NZ: 512 512 1 MODE : real