CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/439.mrc Output diagnostic file name Micrographs_0601/439.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/439.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2414.000 8534.000 Mean, RMS : 4490.654 219.9830 TITLE 1: emi2mrc: 439.emi Mon Jan 06 14:46:48 2014 TITLE 2: kV 300, Mag 78000x, Def 17560, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 287.249, Y 848.757, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01009 10200.00 10000.00 0.00 -0.01000 10400.00 10000.00 0.00 -0.00995 10600.00 10000.00 0.00 -0.00988 10800.00 10000.00 0.00 -0.00963 11000.00 10000.00 0.00 -0.00953 11600.00 10000.00 0.00 -0.00937 11800.00 10000.00 0.00 -0.00911 12000.00 10000.00 0.00 -0.00906 12200.00 10000.00 0.00 -0.00898 12400.00 10000.00 0.00 -0.00864 12600.00 10000.00 0.00 -0.00834 12800.00 10000.00 0.00 -0.00817 13000.00 10000.00 0.00 -0.00795 13200.00 10000.00 0.00 -0.00766 13400.00 10000.00 0.00 -0.00725 13600.00 10000.00 0.00 -0.00679 13800.00 10000.00 0.00 -0.00637 14000.00 10000.00 0.00 -0.00587 14200.00 10000.00 0.00 -0.00523 14400.00 10000.00 0.00 -0.00436 14600.00 10000.00 0.00 -0.00344 14800.00 10000.00 0.00 -0.00256 15000.00 10000.00 0.00 -0.00162 15200.00 10000.00 0.00 -0.00079 15400.00 10000.00 0.00 0.00005 15600.00 10000.00 0.00 0.00107 15800.00 10000.00 0.00 0.00217 16000.00 10000.00 0.00 0.00351 16200.00 10000.00 0.00 0.00504 16400.00 10000.00 0.00 0.00641 16600.00 10000.00 0.00 0.00762 16800.00 10000.00 0.00 0.00881 17000.00 10000.00 0.00 0.01002 17200.00 10000.00 0.00 0.01140 17400.00 10000.00 0.00 0.01284 17600.00 10000.00 0.00 0.01415 17800.00 10000.00 0.00 0.01538 18000.00 10000.00 0.00 0.01658 18200.00 10000.00 0.00 0.01781 18400.00 10000.00 0.00 0.01925 18600.00 10000.00 0.00 0.02072 18800.00 10000.00 0.00 0.02206 19000.00 10000.00 0.00 0.02355 19200.00 10000.00 0.00 0.02522 19400.00 10000.00 0.00 0.02700 19600.00 10000.00 0.00 0.02899 19800.00 10000.00 0.00 0.03117 20000.00 10000.00 0.00 0.03355 20200.00 10000.00 0.00 0.03593 20400.00 10000.00 0.00 0.03825 20600.00 10000.00 0.00 0.04068 20800.00 10000.00 0.00 0.04261 21000.00 10000.00 0.00 0.04361 21200.00 10000.00 0.00 0.04398 21400.00 10000.00 0.00 0.04412 21200.00 10200.00 0.00 0.04443 21400.00 10200.00 0.00 0.04458 21200.00 10400.00 0.00 0.04501 21400.00 10400.00 0.00 0.04516 21200.00 10600.00 0.00 0.04556 21400.00 10600.00 0.00 0.04566 21000.00 10800.00 0.00 0.04576 21200.00 10800.00 0.00 0.04612 21400.00 10800.00 0.00 0.04618 21000.00 11000.00 0.00 0.04643 21200.00 11000.00 0.00 0.04678 21400.00 11000.00 0.00 0.04688 21000.00 11200.00 0.00 0.04717 21200.00 11200.00 0.00 0.04756 21400.00 11200.00 0.00 0.04769 21000.00 11400.00 0.00 0.04809 21200.00 11400.00 0.00 0.04849 21400.00 11400.00 0.00 0.04859 22000.00 11400.00 0.00 0.04864 21000.00 11600.00 0.00 0.04887 21200.00 11600.00 0.00 0.04924 21400.00 11600.00 0.00 0.04931 21800.00 11600.00 0.00 0.04937 22000.00 11600.00 0.00 0.04944 21000.00 11800.00 0.00 0.04970 21200.00 11800.00 0.00 0.05004 21400.00 11800.00 0.00 0.05012 21600.00 11800.00 0.00 0.05017 21800.00 11800.00 0.00 0.05027 21000.00 12000.00 0.00 0.05061 21200.00 12000.00 0.00 0.05095 21400.00 12000.00 0.00 0.05107 21600.00 12000.00 0.00 0.05115 21800.00 12000.00 0.00 0.05121 21000.00 12200.00 0.00 0.05153 21200.00 12200.00 0.00 0.05188 21400.00 12200.00 0.00 0.05204 21600.00 12200.00 0.00 0.05212 21000.00 12400.00 0.00 0.05261 21200.00 12400.00 0.00 0.05298 21400.00 12400.00 0.00 0.05314 21600.00 12400.00 0.00 0.05318 21000.00 12600.00 0.00 0.05379 21200.00 12600.00 0.00 0.05413 21400.00 12600.00 0.00 0.05427 21000.00 12800.00 0.00 0.05494 21200.00 12800.00 0.00 0.05527 21400.00 12800.00 0.00 0.05539 21000.00 13000.00 0.00 0.05596 21200.00 13000.00 0.00 0.05630 21400.00 13000.00 0.00 0.05642 21000.00 13200.00 0.00 0.05710 21200.00 13200.00 0.00 0.05744 21400.00 13200.00 0.00 0.05751 21000.00 13400.00 0.00 0.05819 21200.00 13400.00 0.00 0.05846 21400.00 13400.00 0.00 0.05846 21000.00 13600.00 0.00 0.05926 21200.00 13600.00 0.00 0.05951 21400.00 13600.00 0.00 0.05954 20800.00 13800.00 0.00 0.05961 21000.00 13800.00 0.00 0.06057 21200.00 13800.00 0.00 0.06089 21400.00 13800.00 0.00 0.06097 20800.00 14000.00 0.00 0.06102 21000.00 14000.00 0.00 0.06202 21200.00 14000.00 0.00 0.06236 21400.00 14000.00 0.00 0.06243 20800.00 14200.00 0.00 0.06252 21000.00 14200.00 0.00 0.06351 21200.00 14200.00 0.00 0.06381 21400.00 14200.00 0.00 0.06385 20800.00 14400.00 0.00 0.06391 21000.00 14400.00 0.00 0.06490 21200.00 14400.00 0.00 0.06520 21400.00 14400.00 0.00 0.06525 20800.00 14600.00 0.00 0.06544 21000.00 14600.00 0.00 0.06644 21200.00 14600.00 0.00 0.06673 21400.00 14600.00 0.00 0.06674 20800.00 14800.00 0.00 0.06691 21000.00 14800.00 0.00 0.06787 21200.00 14800.00 0.00 0.06809 20800.00 15000.00 0.00 0.06835 21000.00 15000.00 0.00 0.06926 21200.00 15000.00 0.00 0.06946 20800.00 15200.00 0.00 0.06990 21000.00 15200.00 0.00 0.07083 21200.00 15200.00 0.00 0.07107 21400.00 15200.00 0.00 0.07107 20800.00 15400.00 0.00 0.07162 21000.00 15400.00 0.00 0.07256 21200.00 15400.00 0.00 0.07281 20800.00 15600.00 0.00 0.07352 21000.00 15600.00 0.00 0.07444 21200.00 15600.00 0.00 0.07469 20800.00 15800.00 0.00 0.07540 21000.00 15800.00 0.00 0.07630 21200.00 15800.00 0.00 0.07650 20800.00 16000.00 0.00 0.07737 21000.00 16000.00 0.00 0.07827 21200.00 16000.00 0.00 0.07843 20800.00 16200.00 0.00 0.07928 21000.00 16200.00 0.00 0.08019 21200.00 16200.00 0.00 0.08029 20800.00 16400.00 0.00 0.08116 21000.00 16400.00 0.00 0.08207 21200.00 16400.00 0.00 0.08209 20800.00 16600.00 0.00 0.08310 21000.00 16600.00 0.00 0.08396 20800.00 16800.00 0.00 0.08512 21000.00 16800.00 0.00 0.08592 20800.00 17000.00 0.00 0.08704 21000.00 17000.00 0.00 0.08779 20800.00 17200.00 0.00 0.08894 21000.00 17200.00 0.00 0.08968 20800.00 17400.00 0.00 0.09105 21000.00 17400.00 0.00 0.09173 20800.00 17600.00 0.00 0.09300 21000.00 17600.00 0.00 0.09362 20800.00 17800.00 0.00 0.09518 21000.00 17800.00 0.00 0.09580 20800.00 18000.00 0.00 0.09751 21000.00 18000.00 0.00 0.09810 20800.00 18200.00 0.00 0.09993 21000.00 18200.00 0.00 0.10045 20800.00 18400.00 0.00 0.10236 21000.00 18400.00 0.00 0.10277 20800.00 18600.00 0.00 0.10469 21000.00 18600.00 0.00 0.10500 20600.00 18800.00 0.00 0.10513 20800.00 18800.00 0.00 0.10725 21000.00 18800.00 0.00 0.10745 20600.00 19000.00 0.00 0.10772 20800.00 19000.00 0.00 0.10977 21000.00 19000.00 0.00 0.10984 20600.00 19200.00 0.00 0.11037 20800.00 19200.00 0.00 0.11237 20600.00 19400.00 0.00 0.11295 20800.00 19400.00 0.00 0.11490 20600.00 19600.00 0.00 0.11574 20800.00 19600.00 0.00 0.11762 20600.00 19800.00 0.00 0.11855 20800.00 19800.00 0.00 0.12034 20600.00 20000.00 0.00 0.12134 20800.00 20000.00 0.00 0.12306 20600.00 20200.00 0.00 0.12419 20800.00 20200.00 0.00 0.12574 20600.00 20400.00 0.00 0.12672 20800.00 20400.00 0.00 0.12794 20600.00 20600.00 0.00 0.12944 20800.00 20600.00 0.00 0.12983 20600.00 20800.00 0.00 0.13061 20600.00 20800.00 5.00 0.13072 20600.00 20800.00 10.00 0.13081 20600.00 20800.00 15.00 0.13089 20600.00 20800.00 20.00 0.13094 20600.00 20800.00 25.00 0.13096 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20643.47 20771.52 27.27 0.13107 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/439.ctf NX, NY, NZ: 512 512 1 MODE : real