CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/438.mrc Output diagnostic file name Micrographs_0601/438.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/438.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2536.000 7970.000 Mean, RMS : 4496.977 221.5582 TITLE 1: emi2mrc: 438.emi Mon Jan 06 14:45:56 2014 TITLE 2: kV 300, Mag 78000x, Def 19690, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 285.488, Y 850.597, Z -24.444, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01499 10200.00 10000.00 0.00 -0.01496 13000.00 10000.00 0.00 -0.01477 13200.00 10000.00 0.00 -0.01438 13400.00 10000.00 0.00 -0.01401 13600.00 10000.00 0.00 -0.01353 13800.00 10000.00 0.00 -0.01307 14000.00 10000.00 0.00 -0.01267 14200.00 10000.00 0.00 -0.01235 14400.00 10000.00 0.00 -0.01197 14600.00 10000.00 0.00 -0.01164 14800.00 10000.00 0.00 -0.01131 15000.00 10000.00 0.00 -0.01084 15200.00 10000.00 0.00 -0.01056 15400.00 10000.00 0.00 -0.01017 15600.00 10000.00 0.00 -0.00954 15800.00 10000.00 0.00 -0.00888 16000.00 10000.00 0.00 -0.00824 16200.00 10000.00 0.00 -0.00757 16400.00 10000.00 0.00 -0.00681 16600.00 10000.00 0.00 -0.00587 16800.00 10000.00 0.00 -0.00469 17000.00 10000.00 0.00 -0.00367 17200.00 10000.00 0.00 -0.00283 17400.00 10000.00 0.00 -0.00182 17600.00 10000.00 0.00 -0.00066 17800.00 10000.00 0.00 0.00039 18000.00 10000.00 0.00 0.00141 18200.00 10000.00 0.00 0.00243 18400.00 10000.00 0.00 0.00344 18600.00 10000.00 0.00 0.00447 18800.00 10000.00 0.00 0.00555 19000.00 10000.00 0.00 0.00679 19200.00 10000.00 0.00 0.00798 19400.00 10000.00 0.00 0.00907 19600.00 10000.00 0.00 0.01030 19800.00 10000.00 0.00 0.01161 20000.00 10000.00 0.00 0.01289 20200.00 10000.00 0.00 0.01414 20400.00 10000.00 0.00 0.01554 20600.00 10000.00 0.00 0.01707 20800.00 10000.00 0.00 0.01847 21000.00 10000.00 0.00 0.01995 21200.00 10000.00 0.00 0.02149 21400.00 10000.00 0.00 0.02292 21600.00 10000.00 0.00 0.02451 21800.00 10000.00 0.00 0.02635 22000.00 10000.00 0.00 0.02830 22200.00 10000.00 0.00 0.03046 22400.00 10000.00 0.00 0.03272 22600.00 10000.00 0.00 0.03458 22800.00 10000.00 0.00 0.03562 23000.00 10000.00 0.00 0.03602 23200.00 10000.00 0.00 0.03622 23400.00 10000.00 0.00 0.03633 23600.00 10000.00 0.00 0.03633 23200.00 10200.00 0.00 0.03646 23400.00 10200.00 0.00 0.03659 23200.00 10400.00 0.00 0.03681 23400.00 10400.00 0.00 0.03695 23200.00 10600.00 0.00 0.03705 23400.00 10600.00 0.00 0.03719 23200.00 10800.00 0.00 0.03730 23400.00 10800.00 0.00 0.03741 23000.00 11000.00 0.00 0.03749 23200.00 11000.00 0.00 0.03772 23400.00 11000.00 0.00 0.03778 23000.00 11200.00 0.00 0.03783 23200.00 11200.00 0.00 0.03806 23400.00 11200.00 0.00 0.03812 23000.00 11400.00 0.00 0.03822 23200.00 11400.00 0.00 0.03850 23400.00 11400.00 0.00 0.03861 23000.00 11600.00 0.00 0.03874 23200.00 11600.00 0.00 0.03908 23400.00 11600.00 0.00 0.03921 23000.00 11800.00 0.00 0.03944 23200.00 11800.00 0.00 0.03979 23400.00 11800.00 0.00 0.03990 23000.00 12000.00 0.00 0.04014 23200.00 12000.00 0.00 0.04048 23400.00 12000.00 0.00 0.04058 23000.00 12200.00 0.00 0.04087 23200.00 12200.00 0.00 0.04118 23400.00 12200.00 0.00 0.04127 23000.00 12400.00 0.00 0.04164 23200.00 12400.00 0.00 0.04194 23400.00 12400.00 0.00 0.04203 23000.00 12600.00 0.00 0.04235 23200.00 12600.00 0.00 0.04265 23400.00 12600.00 0.00 0.04273 23000.00 12800.00 0.00 0.04317 23200.00 12800.00 0.00 0.04346 23400.00 12800.00 0.00 0.04351 23000.00 13000.00 0.00 0.04401 23200.00 13000.00 0.00 0.04430 23400.00 13000.00 0.00 0.04438 22800.00 13200.00 0.00 0.04454 23000.00 13200.00 0.00 0.04505 23200.00 13200.00 0.00 0.04537 23400.00 13200.00 0.00 0.04549 22800.00 13400.00 0.00 0.04565 23000.00 13400.00 0.00 0.04614 23200.00 13400.00 0.00 0.04647 23400.00 13400.00 0.00 0.04657 22800.00 13600.00 0.00 0.04664 23000.00 13600.00 0.00 0.04713 23200.00 13600.00 0.00 0.04746 23400.00 13600.00 0.00 0.04749 22800.00 13800.00 0.00 0.04764 23000.00 13800.00 0.00 0.04815 23200.00 13800.00 0.00 0.04845 22800.00 14000.00 0.00 0.04853 23000.00 14000.00 0.00 0.04902 23200.00 14000.00 0.00 0.04928 22800.00 14200.00 0.00 0.04936 23000.00 14200.00 0.00 0.04986 23200.00 14200.00 0.00 0.05010 22800.00 14400.00 0.00 0.05021 23000.00 14400.00 0.00 0.05070 23200.00 14400.00 0.00 0.05090 22800.00 14600.00 0.00 0.05118 23000.00 14600.00 0.00 0.05164 23200.00 14600.00 0.00 0.05179 22800.00 14800.00 0.00 0.05219 23000.00 14800.00 0.00 0.05262 23200.00 14800.00 0.00 0.05275 22800.00 15000.00 0.00 0.05319 23000.00 15000.00 0.00 0.05360 23200.00 15000.00 0.00 0.05374 22800.00 15200.00 0.00 0.05423 23000.00 15200.00 0.00 0.05467 23200.00 15200.00 0.00 0.05484 22800.00 15400.00 0.00 0.05541 23000.00 15400.00 0.00 0.05589 23200.00 15400.00 0.00 0.05605 22800.00 15600.00 0.00 0.05679 23000.00 15600.00 0.00 0.05729 23200.00 15600.00 0.00 0.05741 22800.00 15800.00 0.00 0.05807 23000.00 15800.00 0.00 0.05855 23200.00 15800.00 0.00 0.05860 22800.00 16000.00 0.00 0.05930 23000.00 16000.00 0.00 0.05973 23200.00 16000.00 0.00 0.05974 22800.00 16200.00 0.00 0.06067 23000.00 16200.00 0.00 0.06109 23200.00 16200.00 0.00 0.06109 22600.00 16400.00 0.00 0.06109 22800.00 16400.00 0.00 0.06226 23000.00 16400.00 0.00 0.06266 23200.00 16400.00 0.00 0.06266 22600.00 16600.00 0.00 0.06291 22800.00 16600.00 0.00 0.06406 23000.00 16600.00 0.00 0.06443 22600.00 16800.00 0.00 0.06476 22800.00 16800.00 0.00 0.06586 23000.00 16800.00 0.00 0.06620 22600.00 17000.00 0.00 0.06642 22800.00 17000.00 0.00 0.06747 23000.00 17000.00 0.00 0.06778 22600.00 17200.00 0.00 0.06801 22800.00 17200.00 0.00 0.06900 23000.00 17200.00 0.00 0.06928 22600.00 17400.00 0.00 0.06957 22800.00 17400.00 0.00 0.07053 23000.00 17400.00 0.00 0.07081 22600.00 17600.00 0.00 0.07109 22800.00 17600.00 0.00 0.07206 23000.00 17600.00 0.00 0.07234 22600.00 17800.00 0.00 0.07263 22800.00 17800.00 0.00 0.07362 23000.00 17800.00 0.00 0.07389 22600.00 18000.00 0.00 0.07415 22800.00 18000.00 0.00 0.07519 23000.00 18000.00 0.00 0.07544 22600.00 18200.00 0.00 0.07589 22800.00 18200.00 0.00 0.07699 23000.00 18200.00 0.00 0.07722 22600.00 18400.00 0.00 0.07765 22800.00 18400.00 0.00 0.07878 23000.00 18400.00 0.00 0.07893 22600.00 18600.00 0.00 0.07940 22800.00 18600.00 0.00 0.08049 23000.00 18600.00 0.00 0.08055 22600.00 18800.00 0.00 0.08123 22800.00 18800.00 0.00 0.08225 22600.00 19000.00 0.00 0.08305 22800.00 19000.00 0.00 0.08401 22600.00 19200.00 0.00 0.08497 22800.00 19200.00 0.00 0.08588 22600.00 19400.00 0.00 0.08677 22800.00 19400.00 0.00 0.08768 22600.00 19600.00 0.00 0.08878 22800.00 19600.00 0.00 0.08972 22600.00 19800.00 0.00 0.09084 22800.00 19800.00 0.00 0.09175 22600.00 20000.00 0.00 0.09310 22800.00 20000.00 0.00 0.09394 22600.00 20200.00 0.00 0.09550 22800.00 20200.00 0.00 0.09624 22600.00 20400.00 0.00 0.09778 22800.00 20400.00 0.00 0.09845 22600.00 20600.00 0.00 0.10036 22800.00 20600.00 0.00 0.10094 22600.00 20800.00 0.00 0.10299 22800.00 20800.00 0.00 0.10348 22400.00 21000.00 0.00 0.10352 22600.00 21000.00 0.00 0.10594 22800.00 21000.00 0.00 0.10632 22400.00 21200.00 0.00 0.10654 22600.00 21200.00 0.00 0.10892 22800.00 21200.00 0.00 0.10915 22400.00 21400.00 0.00 0.10972 22600.00 21400.00 0.00 0.11204 22800.00 21400.00 0.00 0.11209 22400.00 21600.00 0.00 0.11316 22600.00 21600.00 0.00 0.11539 22400.00 21800.00 0.00 0.11668 22600.00 21800.00 0.00 0.11873 22400.00 22000.00 0.00 0.12040 22600.00 22000.00 0.00 0.12219 22400.00 22200.00 0.00 0.12383 22600.00 22200.00 0.00 0.12537 22400.00 22400.00 0.00 0.12780 22600.00 22400.00 0.00 0.12872 22400.00 22600.00 0.00 0.13022 22400.00 22600.00 5.00 0.13037 22400.00 22600.00 10.00 0.13050 22400.00 22600.00 15.00 0.13059 22400.00 22600.00 20.00 0.13065 22400.00 22600.00 25.00 0.13068 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22455.97 22619.73 25.64 0.13091 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/438.ctf NX, NY, NZ: 512 512 1 MODE : real