CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/436.mrc Output diagnostic file name Micrographs_0601/436.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/436.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2288.000 7656.000 Mean, RMS : 4438.346 227.7712 TITLE 1: emi2mrc: 436.emi Mon Jan 06 14:42:22 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.06%, Obj 88.08%, Dif 73.67% TITLE 3: X 281.748, Y 858.809, Z -25.199, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01607 11600.00 10000.00 0.00 -0.01603 11800.00 10000.00 0.00 -0.01594 12000.00 10000.00 0.00 -0.01584 12200.00 10000.00 0.00 -0.01579 12400.00 10000.00 0.00 -0.01568 12600.00 10000.00 0.00 -0.01541 12800.00 10000.00 0.00 -0.01520 13000.00 10000.00 0.00 -0.01495 13200.00 10000.00 0.00 -0.01463 13400.00 10000.00 0.00 -0.01428 13600.00 10000.00 0.00 -0.01382 13800.00 10000.00 0.00 -0.01348 14000.00 10000.00 0.00 -0.01317 14200.00 10000.00 0.00 -0.01292 14400.00 10000.00 0.00 -0.01262 14600.00 10000.00 0.00 -0.01223 14800.00 10000.00 0.00 -0.01189 15000.00 10000.00 0.00 -0.01133 15200.00 10000.00 0.00 -0.01065 15400.00 10000.00 0.00 -0.01000 15600.00 10000.00 0.00 -0.00923 15800.00 10000.00 0.00 -0.00843 16000.00 10000.00 0.00 -0.00770 16200.00 10000.00 0.00 -0.00688 16400.00 10000.00 0.00 -0.00597 16600.00 10000.00 0.00 -0.00508 16800.00 10000.00 0.00 -0.00422 17000.00 10000.00 0.00 -0.00344 17200.00 10000.00 0.00 -0.00252 17400.00 10000.00 0.00 -0.00156 17600.00 10000.00 0.00 -0.00070 17800.00 10000.00 0.00 0.00024 18000.00 10000.00 0.00 0.00124 18200.00 10000.00 0.00 0.00223 18400.00 10000.00 0.00 0.00314 18600.00 10000.00 0.00 0.00406 18800.00 10000.00 0.00 0.00504 19000.00 10000.00 0.00 0.00608 19200.00 10000.00 0.00 0.00719 19400.00 10000.00 0.00 0.00836 19600.00 10000.00 0.00 0.00960 19800.00 10000.00 0.00 0.01067 20000.00 10000.00 0.00 0.01164 20200.00 10000.00 0.00 0.01274 20400.00 10000.00 0.00 0.01390 20600.00 10000.00 0.00 0.01528 20800.00 10000.00 0.00 0.01677 21000.00 10000.00 0.00 0.01824 21200.00 10000.00 0.00 0.01968 21400.00 10000.00 0.00 0.02116 21600.00 10000.00 0.00 0.02276 21800.00 10000.00 0.00 0.02428 22000.00 10000.00 0.00 0.02576 22200.00 10000.00 0.00 0.02725 22400.00 10000.00 0.00 0.02884 22600.00 10000.00 0.00 0.03064 22800.00 10000.00 0.00 0.03253 23000.00 10000.00 0.00 0.03436 23200.00 10000.00 0.00 0.03576 23400.00 10000.00 0.00 0.03659 23600.00 10000.00 0.00 0.03700 23800.00 10000.00 0.00 0.03720 24000.00 10000.00 0.00 0.03721 23600.00 10200.00 0.00 0.03755 23800.00 10200.00 0.00 0.03777 23600.00 10400.00 0.00 0.03803 23800.00 10400.00 0.00 0.03823 23600.00 10600.00 0.00 0.03844 23800.00 10600.00 0.00 0.03857 23600.00 10800.00 0.00 0.03870 23800.00 10800.00 0.00 0.03877 23600.00 11000.00 0.00 0.03904 23800.00 11000.00 0.00 0.03909 23400.00 11200.00 0.00 0.03914 23600.00 11200.00 0.00 0.03957 23800.00 11200.00 0.00 0.03960 23400.00 11400.00 0.00 0.03988 23600.00 11400.00 0.00 0.04029 23400.00 11600.00 0.00 0.04056 23600.00 11600.00 0.00 0.04095 23400.00 11800.00 0.00 0.04112 23600.00 11800.00 0.00 0.04148 23400.00 12000.00 0.00 0.04167 23600.00 12000.00 0.00 0.04200 23400.00 12200.00 0.00 0.04221 23600.00 12200.00 0.00 0.04252 23400.00 12400.00 0.00 0.04278 23600.00 12400.00 0.00 0.04311 23400.00 12600.00 0.00 0.04346 23600.00 12600.00 0.00 0.04380 23400.00 12800.00 0.00 0.04430 23600.00 12800.00 0.00 0.04461 23400.00 13000.00 0.00 0.04507 23600.00 13000.00 0.00 0.04533 23400.00 13200.00 0.00 0.04577 23600.00 13200.00 0.00 0.04601 23400.00 13400.00 0.00 0.04661 23600.00 13400.00 0.00 0.04684 23400.00 13600.00 0.00 0.04746 23600.00 13600.00 0.00 0.04766 23400.00 13800.00 0.00 0.04820 23600.00 13800.00 0.00 0.04841 23400.00 14000.00 0.00 0.04900 23600.00 14000.00 0.00 0.04922 23400.00 14200.00 0.00 0.04992 23600.00 14200.00 0.00 0.05012 23400.00 14400.00 0.00 0.05090 23600.00 14400.00 0.00 0.05104 23200.00 14600.00 0.00 0.05108 23400.00 14600.00 0.00 0.05181 23600.00 14600.00 0.00 0.05190 23200.00 14800.00 0.00 0.05194 23400.00 14800.00 0.00 0.05265 23600.00 14800.00 0.00 0.05279 23200.00 15000.00 0.00 0.05300 23400.00 15000.00 0.00 0.05375 23600.00 15000.00 0.00 0.05393 23200.00 15200.00 0.00 0.05409 23400.00 15200.00 0.00 0.05485 23600.00 15200.00 0.00 0.05500 23200.00 15400.00 0.00 0.05514 23400.00 15400.00 0.00 0.05589 23600.00 15400.00 0.00 0.05604 23200.00 15600.00 0.00 0.05628 23400.00 15600.00 0.00 0.05705 23600.00 15600.00 0.00 0.05722 23200.00 15800.00 0.00 0.05735 23400.00 15800.00 0.00 0.05815 23600.00 15800.00 0.00 0.05832 23200.00 16000.00 0.00 0.05860 23400.00 16000.00 0.00 0.05941 23600.00 16000.00 0.00 0.05954 23200.00 16200.00 0.00 0.05980 23400.00 16200.00 0.00 0.06057 23600.00 16200.00 0.00 0.06062 23200.00 16400.00 0.00 0.06085 23400.00 16400.00 0.00 0.06160 23200.00 16600.00 0.00 0.06202 23400.00 16600.00 0.00 0.06276 23200.00 16800.00 0.00 0.06324 23400.00 16800.00 0.00 0.06393 23200.00 17000.00 0.00 0.06443 23400.00 17000.00 0.00 0.06509 23200.00 17200.00 0.00 0.06571 23400.00 17200.00 0.00 0.06637 23200.00 17400.00 0.00 0.06714 23400.00 17400.00 0.00 0.06779 23200.00 17600.00 0.00 0.06854 23400.00 17600.00 0.00 0.06915 23200.00 17800.00 0.00 0.06996 23400.00 17800.00 0.00 0.07057 23200.00 18000.00 0.00 0.07151 23400.00 18000.00 0.00 0.07213 23200.00 18200.00 0.00 0.07310 23400.00 18200.00 0.00 0.07374 23200.00 18400.00 0.00 0.07470 23400.00 18400.00 0.00 0.07536 23600.00 18400.00 0.00 0.07537 23200.00 18600.00 0.00 0.07644 23400.00 18600.00 0.00 0.07713 23600.00 18600.00 0.00 0.07715 23200.00 18800.00 0.00 0.07822 23400.00 18800.00 0.00 0.07893 23200.00 19000.00 0.00 0.07999 23400.00 19000.00 0.00 0.08071 23200.00 19200.00 0.00 0.08200 23400.00 19200.00 0.00 0.08271 23200.00 19400.00 0.00 0.08402 23400.00 19400.00 0.00 0.08471 23200.00 19600.00 0.00 0.08610 23400.00 19600.00 0.00 0.08672 23200.00 19800.00 0.00 0.08809 23400.00 19800.00 0.00 0.08859 23200.00 20000.00 0.00 0.09000 23400.00 20000.00 0.00 0.09041 23000.00 20200.00 0.00 0.09042 23200.00 20200.00 0.00 0.09200 23400.00 20200.00 0.00 0.09234 23000.00 20400.00 0.00 0.09241 23200.00 20400.00 0.00 0.09395 23400.00 20400.00 0.00 0.09421 23000.00 20600.00 0.00 0.09451 23200.00 20600.00 0.00 0.09601 23400.00 20600.00 0.00 0.09622 23000.00 20800.00 0.00 0.09676 23200.00 20800.00 0.00 0.09824 23400.00 20800.00 0.00 0.09838 23000.00 21000.00 0.00 0.09925 23200.00 21000.00 0.00 0.10065 23400.00 21000.00 0.00 0.10065 23000.00 21200.00 0.00 0.10162 23200.00 21200.00 0.00 0.10291 23000.00 21400.00 0.00 0.10404 23200.00 21400.00 0.00 0.10521 23000.00 21600.00 0.00 0.10637 23200.00 21600.00 0.00 0.10742 23000.00 21800.00 0.00 0.10862 23200.00 21800.00 0.00 0.10958 23000.00 22000.00 0.00 0.11118 23200.00 22000.00 0.00 0.11203 23000.00 22200.00 0.00 0.11382 23200.00 22200.00 0.00 0.11457 23000.00 22400.00 0.00 0.11679 23200.00 22400.00 0.00 0.11729 23000.00 22600.00 0.00 0.11947 23200.00 22600.00 0.00 0.11961 22800.00 22800.00 0.00 0.11992 23000.00 22800.00 0.00 0.12218 22800.00 23000.00 0.00 0.12238 23000.00 23000.00 0.00 0.12390 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22935.84 23070.84 37.52 0.12417 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/436.ctf NX, NY, NZ: 512 512 1 MODE : real