CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/433.mrc Output diagnostic file name Micrographs_0601/433.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/433.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1798.000 8286.000 Mean, RMS : 4427.390 215.8247 TITLE 1: emi2mrc: 433.emi Mon Jan 06 14:38:56 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.06%, Obj 88.09%, Dif 73.67% TITLE 3: X 287.237, Y 852.842, Z -25.199, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01490 10200.00 10000.00 0.00 0.01575 10400.00 10000.00 0.00 0.01653 10600.00 10000.00 0.00 0.01749 10800.00 10000.00 0.00 0.01855 11000.00 10000.00 0.00 0.01960 11200.00 10000.00 0.00 0.02069 11400.00 10000.00 0.00 0.02198 11600.00 10000.00 0.00 0.02343 11800.00 10000.00 0.00 0.02486 12000.00 10000.00 0.00 0.02648 12200.00 10000.00 0.00 0.02830 12400.00 10000.00 0.00 0.03024 12600.00 10000.00 0.00 0.03231 12800.00 10000.00 0.00 0.03459 13000.00 10000.00 0.00 0.03693 13200.00 10000.00 0.00 0.03945 13400.00 10000.00 0.00 0.04232 13600.00 10000.00 0.00 0.04521 13800.00 10000.00 0.00 0.04816 14000.00 10000.00 0.00 0.05102 14200.00 10000.00 0.00 0.05390 14400.00 10000.00 0.00 0.05677 14600.00 10000.00 0.00 0.05972 14800.00 10000.00 0.00 0.06309 15000.00 10000.00 0.00 0.06670 15200.00 10000.00 0.00 0.07070 15400.00 10000.00 0.00 0.07450 15600.00 10000.00 0.00 0.07724 15800.00 10000.00 0.00 0.07865 16000.00 10000.00 0.00 0.07910 15800.00 10200.00 0.00 0.08002 16000.00 10200.00 0.00 0.08043 15800.00 10400.00 0.00 0.08158 16000.00 10400.00 0.00 0.08197 15800.00 10600.00 0.00 0.08337 16000.00 10600.00 0.00 0.08373 15800.00 10800.00 0.00 0.08513 16000.00 10800.00 0.00 0.08546 15600.00 11000.00 0.00 0.08570 15800.00 11000.00 0.00 0.08715 16000.00 11000.00 0.00 0.08746 15600.00 11200.00 0.00 0.08770 15800.00 11200.00 0.00 0.08919 16000.00 11200.00 0.00 0.08946 15600.00 11400.00 0.00 0.09005 15800.00 11400.00 0.00 0.09154 16000.00 11400.00 0.00 0.09173 15600.00 11600.00 0.00 0.09241 15800.00 11600.00 0.00 0.09385 16000.00 11600.00 0.00 0.09391 15600.00 11800.00 0.00 0.09477 15800.00 11800.00 0.00 0.09612 15600.00 12000.00 0.00 0.09741 15800.00 12000.00 0.00 0.09869 15600.00 12200.00 0.00 0.10041 15800.00 12200.00 0.00 0.10163 15600.00 12400.00 0.00 0.10370 15800.00 12400.00 0.00 0.10486 15600.00 12600.00 0.00 0.10694 15800.00 12600.00 0.00 0.10798 15600.00 12800.00 0.00 0.11034 15800.00 12800.00 0.00 0.11124 15600.00 13000.00 0.00 0.11351 15800.00 13000.00 0.00 0.11431 15600.00 13200.00 0.00 0.11700 15800.00 13200.00 0.00 0.11773 15600.00 13400.00 0.00 0.12072 15800.00 13400.00 0.00 0.12129 15400.00 13600.00 0.00 0.12149 15600.00 13600.00 0.00 0.12447 15800.00 13600.00 0.00 0.12486 15400.00 13800.00 0.00 0.12543 15600.00 13800.00 0.00 0.12834 15800.00 13800.00 0.00 0.12855 15400.00 14000.00 0.00 0.12929 15600.00 14000.00 0.00 0.13208 15800.00 14000.00 0.00 0.13210 15400.00 14200.00 0.00 0.13341 15600.00 14200.00 0.00 0.13609 15400.00 14400.00 0.00 0.13746 15600.00 14400.00 0.00 0.14000 15400.00 14600.00 0.00 0.14209 15600.00 14600.00 0.00 0.14440 15400.00 14800.00 0.00 0.14679 15600.00 14800.00 0.00 0.14881 15400.00 15000.00 0.00 0.15173 15600.00 15000.00 0.00 0.15337 15400.00 15200.00 0.00 0.15683 15600.00 15200.00 0.00 0.15787 15400.00 15400.00 0.00 0.16113 15600.00 15400.00 0.00 0.16122 15400.00 15600.00 0.00 0.16321 15400.00 15600.00 5.00 0.16328 15400.00 15600.00 10.00 0.16332 15400.00 15600.00 15.00 0.16332 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15438.93 15573.73 13.21 0.16341 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/433.ctf NX, NY, NZ: 512 512 1 MODE : real