CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/430.mrc Output diagnostic file name Micrographs_0601/430.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/430.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2520.000 8744.000 Mean, RMS : 4488.715 224.2367 TITLE 1: emi2mrc: 430.emi Mon Jan 06 14:34:56 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 294.180, Y 845.038, Z -24.919, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01692 12000.00 10000.00 0.00 -0.01682 12200.00 10000.00 0.00 -0.01661 12400.00 10000.00 0.00 -0.01653 12600.00 10000.00 0.00 -0.01638 12800.00 10000.00 0.00 -0.01631 13000.00 10000.00 0.00 -0.01626 13200.00 10000.00 0.00 -0.01601 13400.00 10000.00 0.00 -0.01580 13600.00 10000.00 0.00 -0.01550 13800.00 10000.00 0.00 -0.01517 14000.00 10000.00 0.00 -0.01487 14200.00 10000.00 0.00 -0.01451 14400.00 10000.00 0.00 -0.01417 14600.00 10000.00 0.00 -0.01386 14800.00 10000.00 0.00 -0.01350 15000.00 10000.00 0.00 -0.01313 15200.00 10000.00 0.00 -0.01285 15400.00 10000.00 0.00 -0.01254 15600.00 10000.00 0.00 -0.01205 15800.00 10000.00 0.00 -0.01149 16000.00 10000.00 0.00 -0.01091 16200.00 10000.00 0.00 -0.01042 16400.00 10000.00 0.00 -0.01003 16600.00 10000.00 0.00 -0.00963 16800.00 10000.00 0.00 -0.00915 17000.00 10000.00 0.00 -0.00848 17200.00 10000.00 0.00 -0.00778 17400.00 10000.00 0.00 -0.00715 17600.00 10000.00 0.00 -0.00655 17800.00 10000.00 0.00 -0.00601 18000.00 10000.00 0.00 -0.00534 18200.00 10000.00 0.00 -0.00456 18400.00 10000.00 0.00 -0.00361 18600.00 10000.00 0.00 -0.00253 18800.00 10000.00 0.00 -0.00149 19000.00 10000.00 0.00 -0.00039 19200.00 10000.00 0.00 0.00072 19400.00 10000.00 0.00 0.00198 19600.00 10000.00 0.00 0.00329 19800.00 10000.00 0.00 0.00440 20000.00 10000.00 0.00 0.00541 20200.00 10000.00 0.00 0.00636 20400.00 10000.00 0.00 0.00732 20600.00 10000.00 0.00 0.00838 20800.00 10000.00 0.00 0.00947 21000.00 10000.00 0.00 0.01057 21200.00 10000.00 0.00 0.01177 21400.00 10000.00 0.00 0.01301 21600.00 10000.00 0.00 0.01427 21800.00 10000.00 0.00 0.01567 22000.00 10000.00 0.00 0.01712 22200.00 10000.00 0.00 0.01868 22400.00 10000.00 0.00 0.02040 22600.00 10000.00 0.00 0.02229 22800.00 10000.00 0.00 0.02436 23000.00 10000.00 0.00 0.02650 23200.00 10000.00 0.00 0.02882 23400.00 10000.00 0.00 0.03088 23600.00 10000.00 0.00 0.03231 23800.00 10000.00 0.00 0.03319 24000.00 10000.00 0.00 0.03362 24200.00 10000.00 0.00 0.03382 24400.00 10000.00 0.00 0.03386 24000.00 10200.00 0.00 0.03393 24200.00 10200.00 0.00 0.03412 24400.00 10200.00 0.00 0.03414 24000.00 10400.00 0.00 0.03416 24200.00 10400.00 0.00 0.03439 24400.00 10400.00 0.00 0.03442 24000.00 10600.00 0.00 0.03461 24200.00 10600.00 0.00 0.03481 24000.00 10800.00 0.00 0.03506 24200.00 10800.00 0.00 0.03520 24000.00 11000.00 0.00 0.03548 24200.00 11000.00 0.00 0.03556 24000.00 11200.00 0.00 0.03585 24200.00 11200.00 0.00 0.03589 24000.00 11400.00 0.00 0.03613 24200.00 11400.00 0.00 0.03618 24400.00 11400.00 0.00 0.03622 23800.00 11600.00 0.00 0.03632 24000.00 11600.00 0.00 0.03658 24200.00 11600.00 0.00 0.03670 24400.00 11600.00 0.00 0.03680 23800.00 11800.00 0.00 0.03683 24000.00 11800.00 0.00 0.03712 24200.00 11800.00 0.00 0.03730 24400.00 11800.00 0.00 0.03740 24000.00 12000.00 0.00 0.03763 24200.00 12000.00 0.00 0.03783 24400.00 12000.00 0.00 0.03791 23800.00 12200.00 0.00 0.03806 24000.00 12200.00 0.00 0.03841 24200.00 12200.00 0.00 0.03862 24400.00 12200.00 0.00 0.03866 23800.00 12400.00 0.00 0.03882 24000.00 12400.00 0.00 0.03917 24200.00 12400.00 0.00 0.03936 24400.00 12400.00 0.00 0.03937 24000.00 12600.00 0.00 0.03972 24200.00 12600.00 0.00 0.03988 23800.00 12800.00 0.00 0.04005 24000.00 12800.00 0.00 0.04037 24200.00 12800.00 0.00 0.04049 23800.00 13000.00 0.00 0.04072 24000.00 13000.00 0.00 0.04100 24200.00 13000.00 0.00 0.04109 23800.00 13200.00 0.00 0.04147 24000.00 13200.00 0.00 0.04174 24200.00 13200.00 0.00 0.04184 23800.00 13400.00 0.00 0.04229 24000.00 13400.00 0.00 0.04256 24200.00 13400.00 0.00 0.04268 23800.00 13600.00 0.00 0.04310 24000.00 13600.00 0.00 0.04339 24200.00 13600.00 0.00 0.04353 23800.00 13800.00 0.00 0.04399 24000.00 13800.00 0.00 0.04430 24200.00 13800.00 0.00 0.04445 23800.00 14000.00 0.00 0.04485 24000.00 14000.00 0.00 0.04519 24200.00 14000.00 0.00 0.04532 23800.00 14200.00 0.00 0.04582 24000.00 14200.00 0.00 0.04614 24200.00 14200.00 0.00 0.04623 23800.00 14400.00 0.00 0.04675 24000.00 14400.00 0.00 0.04706 24200.00 14400.00 0.00 0.04713 23800.00 14600.00 0.00 0.04771 24000.00 14600.00 0.00 0.04799 24200.00 14600.00 0.00 0.04804 23800.00 14800.00 0.00 0.04873 24000.00 14800.00 0.00 0.04898 24200.00 14800.00 0.00 0.04899 23600.00 15000.00 0.00 0.04902 23800.00 15000.00 0.00 0.04968 24000.00 15000.00 0.00 0.04988 24200.00 15000.00 0.00 0.04990 23800.00 15200.00 0.00 0.05053 24000.00 15200.00 0.00 0.05074 24200.00 15200.00 0.00 0.05081 23800.00 15400.00 0.00 0.05142 24000.00 15400.00 0.00 0.05168 24200.00 15400.00 0.00 0.05180 23800.00 15600.00 0.00 0.05246 24000.00 15600.00 0.00 0.05277 24200.00 15600.00 0.00 0.05290 23800.00 15800.00 0.00 0.05359 24000.00 15800.00 0.00 0.05392 24200.00 15800.00 0.00 0.05401 23600.00 16000.00 0.00 0.05402 23800.00 16000.00 0.00 0.05471 24000.00 16000.00 0.00 0.05500 24200.00 16000.00 0.00 0.05504 23600.00 16200.00 0.00 0.05510 23800.00 16200.00 0.00 0.05578 24000.00 16200.00 0.00 0.05608 24200.00 16200.00 0.00 0.05612 23600.00 16400.00 0.00 0.05641 23800.00 16400.00 0.00 0.05711 24000.00 16400.00 0.00 0.05741 24200.00 16400.00 0.00 0.05746 23600.00 16600.00 0.00 0.05770 23800.00 16600.00 0.00 0.05840 24000.00 16600.00 0.00 0.05868 23600.00 16800.00 0.00 0.05886 23800.00 16800.00 0.00 0.05957 24000.00 16800.00 0.00 0.05984 23600.00 17000.00 0.00 0.06010 23800.00 17000.00 0.00 0.06084 24000.00 17000.00 0.00 0.06112 23600.00 17200.00 0.00 0.06144 23800.00 17200.00 0.00 0.06221 24000.00 17200.00 0.00 0.06250 23600.00 17400.00 0.00 0.06299 23800.00 17400.00 0.00 0.06376 24000.00 17400.00 0.00 0.06401 23600.00 17600.00 0.00 0.06451 23800.00 17600.00 0.00 0.06523 24000.00 17600.00 0.00 0.06542 23600.00 17800.00 0.00 0.06609 23800.00 17800.00 0.00 0.06677 24000.00 17800.00 0.00 0.06694 23600.00 18000.00 0.00 0.06768 23800.00 18000.00 0.00 0.06835 24000.00 18000.00 0.00 0.06853 23600.00 18200.00 0.00 0.06927 23800.00 18200.00 0.00 0.06992 24000.00 18200.00 0.00 0.07006 23600.00 18400.00 0.00 0.07091 23800.00 18400.00 0.00 0.07153 24000.00 18400.00 0.00 0.07161 23600.00 18600.00 0.00 0.07247 23800.00 18600.00 0.00 0.07307 24000.00 18600.00 0.00 0.07312 23600.00 18800.00 0.00 0.07412 23800.00 18800.00 0.00 0.07472 24000.00 18800.00 0.00 0.07473 23600.00 19000.00 0.00 0.07572 23800.00 19000.00 0.00 0.07629 23600.00 19200.00 0.00 0.07726 23800.00 19200.00 0.00 0.07778 23600.00 19400.00 0.00 0.07887 23800.00 19400.00 0.00 0.07934 23600.00 19600.00 0.00 0.08061 23800.00 19600.00 0.00 0.08101 23600.00 19800.00 0.00 0.08233 23800.00 19800.00 0.00 0.08262 23600.00 20000.00 0.00 0.08387 23800.00 20000.00 0.00 0.08409 23400.00 20200.00 0.00 0.08421 23600.00 20200.00 0.00 0.08545 23800.00 20200.00 0.00 0.08564 23400.00 20400.00 0.00 0.08594 23600.00 20400.00 0.00 0.08715 23800.00 20400.00 0.00 0.08729 23400.00 20600.00 0.00 0.08782 23600.00 20600.00 0.00 0.08900 23800.00 20600.00 0.00 0.08908 23400.00 20800.00 0.00 0.08977 23600.00 20800.00 0.00 0.09094 23800.00 20800.00 0.00 0.09100 23400.00 21000.00 0.00 0.09184 23600.00 21000.00 0.00 0.09303 23800.00 21000.00 0.00 0.09303 23400.00 21200.00 0.00 0.09403 23600.00 21200.00 0.00 0.09521 23400.00 21400.00 0.00 0.09648 23600.00 21400.00 0.00 0.09760 23400.00 21600.00 0.00 0.09904 23600.00 21600.00 0.00 0.10003 23400.00 21800.00 0.00 0.10163 23600.00 21800.00 0.00 0.10249 23400.00 22000.00 0.00 0.10433 23600.00 22000.00 0.00 0.10503 23400.00 22200.00 0.00 0.10706 23600.00 22200.00 0.00 0.10756 23400.00 22400.00 0.00 0.10987 23600.00 22400.00 0.00 0.11010 23200.00 22600.00 0.00 0.11028 23400.00 22600.00 0.00 0.11252 23200.00 22800.00 0.00 0.11330 23400.00 22800.00 0.00 0.11531 23200.00 23000.00 0.00 0.11606 23400.00 23000.00 0.00 0.11779 23200.00 23200.00 0.00 0.11848 23400.00 23200.00 0.00 0.11986 23200.00 23400.00 0.00 0.12013 23400.00 23400.00 0.00 0.12080 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23296.22 23451.10 33.56 0.12115 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/430.ctf NX, NY, NZ: 512 512 1 MODE : real