CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/429.mrc Output diagnostic file name Micrographs_0601/429.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/429.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2420.000 8496.000 Mean, RMS : 4494.305 221.3466 TITLE 1: emi2mrc: 429.emi Mon Jan 06 14:33:21 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 298.104, Y 844.366, Z -24.919, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01545 10600.00 10000.00 0.00 -0.01530 10800.00 10000.00 0.00 -0.01513 11000.00 10000.00 0.00 -0.01496 11200.00 10000.00 0.00 -0.01484 11400.00 10000.00 0.00 -0.01465 11600.00 10000.00 0.00 -0.01438 11800.00 10000.00 0.00 -0.01412 12000.00 10000.00 0.00 -0.01386 12200.00 10000.00 0.00 -0.01356 12400.00 10000.00 0.00 -0.01339 12600.00 10000.00 0.00 -0.01329 12800.00 10000.00 0.00 -0.01296 13000.00 10000.00 0.00 -0.01255 13200.00 10000.00 0.00 -0.01224 13400.00 10000.00 0.00 -0.01178 13600.00 10000.00 0.00 -0.01131 13800.00 10000.00 0.00 -0.01088 14000.00 10000.00 0.00 -0.01028 14200.00 10000.00 0.00 -0.00962 14400.00 10000.00 0.00 -0.00899 14600.00 10000.00 0.00 -0.00845 14800.00 10000.00 0.00 -0.00784 15000.00 10000.00 0.00 -0.00711 15200.00 10000.00 0.00 -0.00629 15400.00 10000.00 0.00 -0.00532 15600.00 10000.00 0.00 -0.00440 15800.00 10000.00 0.00 -0.00351 16000.00 10000.00 0.00 -0.00253 16200.00 10000.00 0.00 -0.00146 16400.00 10000.00 0.00 -0.00031 16600.00 10000.00 0.00 0.00082 16800.00 10000.00 0.00 0.00194 17000.00 10000.00 0.00 0.00307 17200.00 10000.00 0.00 0.00416 17400.00 10000.00 0.00 0.00529 17600.00 10000.00 0.00 0.00635 17800.00 10000.00 0.00 0.00721 18000.00 10000.00 0.00 0.00810 18200.00 10000.00 0.00 0.00912 18400.00 10000.00 0.00 0.01013 18600.00 10000.00 0.00 0.01112 18800.00 10000.00 0.00 0.01213 19000.00 10000.00 0.00 0.01307 19200.00 10000.00 0.00 0.01403 19400.00 10000.00 0.00 0.01510 19600.00 10000.00 0.00 0.01631 19800.00 10000.00 0.00 0.01778 20000.00 10000.00 0.00 0.01943 20200.00 10000.00 0.00 0.02108 20400.00 10000.00 0.00 0.02272 20600.00 10000.00 0.00 0.02459 20800.00 10000.00 0.00 0.02666 21000.00 10000.00 0.00 0.02875 21200.00 10000.00 0.00 0.03097 21400.00 10000.00 0.00 0.03326 21600.00 10000.00 0.00 0.03536 21800.00 10000.00 0.00 0.03700 22000.00 10000.00 0.00 0.03789 22200.00 10000.00 0.00 0.03815 22400.00 10000.00 0.00 0.03828 22600.00 10000.00 0.00 0.03829 22000.00 10200.00 0.00 0.03831 22200.00 10200.00 0.00 0.03862 22400.00 10200.00 0.00 0.03883 22600.00 10200.00 0.00 0.03890 22000.00 10400.00 0.00 0.03897 22200.00 10400.00 0.00 0.03936 22400.00 10400.00 0.00 0.03962 22600.00 10400.00 0.00 0.03969 22000.00 10600.00 0.00 0.03970 22200.00 10600.00 0.00 0.04013 22400.00 10600.00 0.00 0.04037 22200.00 10800.00 0.00 0.04078 22400.00 10800.00 0.00 0.04096 22200.00 11000.00 0.00 0.04128 22400.00 11000.00 0.00 0.04141 22200.00 11200.00 0.00 0.04165 22400.00 11200.00 0.00 0.04177 22200.00 11400.00 0.00 0.04218 22400.00 11400.00 0.00 0.04230 22000.00 11600.00 0.00 0.04233 22200.00 11600.00 0.00 0.04276 22400.00 11600.00 0.00 0.04289 22600.00 11600.00 0.00 0.04293 22000.00 11800.00 0.00 0.04304 22200.00 11800.00 0.00 0.04347 22400.00 11800.00 0.00 0.04362 22600.00 11800.00 0.00 0.04368 22000.00 12000.00 0.00 0.04394 22200.00 12000.00 0.00 0.04436 22400.00 12000.00 0.00 0.04453 22600.00 12000.00 0.00 0.04459 22000.00 12200.00 0.00 0.04485 22200.00 12200.00 0.00 0.04526 22400.00 12200.00 0.00 0.04544 22600.00 12200.00 0.00 0.04549 22000.00 12400.00 0.00 0.04576 22200.00 12400.00 0.00 0.04616 22400.00 12400.00 0.00 0.04635 22600.00 12400.00 0.00 0.04636 22000.00 12600.00 0.00 0.04667 22200.00 12600.00 0.00 0.04706 22400.00 12600.00 0.00 0.04723 22000.00 12800.00 0.00 0.04773 22200.00 12800.00 0.00 0.04811 22400.00 12800.00 0.00 0.04826 22000.00 13000.00 0.00 0.04881 22200.00 13000.00 0.00 0.04915 22400.00 13000.00 0.00 0.04926 22000.00 13200.00 0.00 0.04967 22200.00 13200.00 0.00 0.04998 22400.00 13200.00 0.00 0.05006 22000.00 13400.00 0.00 0.05049 22200.00 13400.00 0.00 0.05081 22400.00 13400.00 0.00 0.05086 22000.00 13600.00 0.00 0.05138 22200.00 13600.00 0.00 0.05169 22000.00 13800.00 0.00 0.05223 22200.00 13800.00 0.00 0.05250 22000.00 14000.00 0.00 0.05318 22200.00 14000.00 0.00 0.05343 22000.00 14200.00 0.00 0.05428 22200.00 14200.00 0.00 0.05452 22000.00 14400.00 0.00 0.05529 22200.00 14400.00 0.00 0.05555 21800.00 14600.00 0.00 0.05557 22000.00 14600.00 0.00 0.05648 22200.00 14600.00 0.00 0.05677 21800.00 14800.00 0.00 0.05689 22000.00 14800.00 0.00 0.05782 22200.00 14800.00 0.00 0.05808 21800.00 15000.00 0.00 0.05820 22000.00 15000.00 0.00 0.05912 22200.00 15000.00 0.00 0.05931 21800.00 15200.00 0.00 0.05948 22000.00 15200.00 0.00 0.06036 22200.00 15200.00 0.00 0.06051 21800.00 15400.00 0.00 0.06079 22000.00 15400.00 0.00 0.06167 22200.00 15400.00 0.00 0.06186 21800.00 15600.00 0.00 0.06223 22000.00 15600.00 0.00 0.06316 22200.00 15600.00 0.00 0.06342 21800.00 15800.00 0.00 0.06380 22000.00 15800.00 0.00 0.06475 22200.00 15800.00 0.00 0.06497 21800.00 16000.00 0.00 0.06542 22000.00 16000.00 0.00 0.06630 22200.00 16000.00 0.00 0.06642 21800.00 16200.00 0.00 0.06685 22000.00 16200.00 0.00 0.06766 22200.00 16200.00 0.00 0.06771 21800.00 16400.00 0.00 0.06837 22000.00 16400.00 0.00 0.06916 22200.00 16400.00 0.00 0.06919 21800.00 16600.00 0.00 0.07003 22000.00 16600.00 0.00 0.07080 22200.00 16600.00 0.00 0.07082 21800.00 16800.00 0.00 0.07173 22000.00 16800.00 0.00 0.07248 22200.00 16800.00 0.00 0.07250 21800.00 17000.00 0.00 0.07351 22000.00 17000.00 0.00 0.07425 22200.00 17000.00 0.00 0.07429 21800.00 17200.00 0.00 0.07525 22000.00 17200.00 0.00 0.07605 22200.00 17200.00 0.00 0.07611 21800.00 17400.00 0.00 0.07711 22000.00 17400.00 0.00 0.07797 22200.00 17400.00 0.00 0.07800 21800.00 17600.00 0.00 0.07902 22000.00 17600.00 0.00 0.07990 21800.00 17800.00 0.00 0.08109 22000.00 17800.00 0.00 0.08193 21800.00 18000.00 0.00 0.08315 22000.00 18000.00 0.00 0.08392 21800.00 18200.00 0.00 0.08525 22000.00 18200.00 0.00 0.08599 21800.00 18400.00 0.00 0.08746 22000.00 18400.00 0.00 0.08816 21800.00 18600.00 0.00 0.08969 22000.00 18600.00 0.00 0.09030 21800.00 18800.00 0.00 0.09192 22000.00 18800.00 0.00 0.09239 21800.00 19000.00 0.00 0.09412 22000.00 19000.00 0.00 0.09448 21600.00 19200.00 0.00 0.09470 21800.00 19200.00 0.00 0.09648 22000.00 19200.00 0.00 0.09674 21600.00 19400.00 0.00 0.09706 21800.00 19400.00 0.00 0.09881 22000.00 19400.00 0.00 0.09898 21600.00 19600.00 0.00 0.09963 21800.00 19600.00 0.00 0.10132 22000.00 19600.00 0.00 0.10136 21600.00 19800.00 0.00 0.10227 21800.00 19800.00 0.00 0.10386 21600.00 20000.00 0.00 0.10492 21800.00 20000.00 0.00 0.10640 21600.00 20200.00 0.00 0.10771 21800.00 20200.00 0.00 0.10906 21600.00 20400.00 0.00 0.11053 21800.00 20400.00 0.00 0.11175 21600.00 20600.00 0.00 0.11354 21800.00 20600.00 0.00 0.11457 21600.00 20800.00 0.00 0.11648 21800.00 20800.00 0.00 0.11728 21600.00 21000.00 0.00 0.11966 21800.00 21000.00 0.00 0.12015 21600.00 21200.00 0.00 0.12253 21800.00 21200.00 0.00 0.12267 21600.00 21400.00 0.00 0.12518 21400.00 21600.00 0.00 0.12527 21600.00 21600.00 0.00 0.12716 21600.00 21800.00 20.00 0.12718 21600.00 21800.00 25.00 0.12719 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21570.19 21702.71 27.28 0.12747 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/429.ctf NX, NY, NZ: 512 512 1 MODE : real