CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/428.mrc Output diagnostic file name Micrographs_0601/428.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/428.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2304.000 8030.000 Mean, RMS : 4472.561 223.0739 TITLE 1: emi2mrc: 428.emi Mon Jan 06 14:32:16 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 296.470, Y 846.251, Z -24.919, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00956 10200.00 10000.00 0.00 -0.00942 10400.00 10000.00 0.00 -0.00921 10600.00 10000.00 0.00 -0.00905 10800.00 10000.00 0.00 -0.00881 11000.00 10000.00 0.00 -0.00849 11200.00 10000.00 0.00 -0.00812 11400.00 10000.00 0.00 -0.00762 11600.00 10000.00 0.00 -0.00718 11800.00 10000.00 0.00 -0.00692 12000.00 10000.00 0.00 -0.00662 12200.00 10000.00 0.00 -0.00621 12400.00 10000.00 0.00 -0.00578 12600.00 10000.00 0.00 -0.00537 12800.00 10000.00 0.00 -0.00502 13000.00 10000.00 0.00 -0.00461 13200.00 10000.00 0.00 -0.00421 13400.00 10000.00 0.00 -0.00387 13600.00 10000.00 0.00 -0.00348 13800.00 10000.00 0.00 -0.00308 14000.00 10000.00 0.00 -0.00261 14200.00 10000.00 0.00 -0.00208 14400.00 10000.00 0.00 -0.00152 14600.00 10000.00 0.00 -0.00085 14800.00 10000.00 0.00 -0.00001 15000.00 10000.00 0.00 0.00077 15200.00 10000.00 0.00 0.00142 15400.00 10000.00 0.00 0.00223 15600.00 10000.00 0.00 0.00322 15800.00 10000.00 0.00 0.00434 16000.00 10000.00 0.00 0.00551 16200.00 10000.00 0.00 0.00672 16400.00 10000.00 0.00 0.00807 16600.00 10000.00 0.00 0.00949 16800.00 10000.00 0.00 0.01098 17000.00 10000.00 0.00 0.01248 17200.00 10000.00 0.00 0.01389 17400.00 10000.00 0.00 0.01527 17600.00 10000.00 0.00 0.01662 17800.00 10000.00 0.00 0.01811 18000.00 10000.00 0.00 0.01960 18200.00 10000.00 0.00 0.02108 18400.00 10000.00 0.00 0.02279 18600.00 10000.00 0.00 0.02455 18800.00 10000.00 0.00 0.02633 19000.00 10000.00 0.00 0.02823 19200.00 10000.00 0.00 0.03017 19400.00 10000.00 0.00 0.03209 19600.00 10000.00 0.00 0.03426 19800.00 10000.00 0.00 0.03657 20000.00 10000.00 0.00 0.03893 20200.00 10000.00 0.00 0.04158 20400.00 10000.00 0.00 0.04394 20600.00 10000.00 0.00 0.04556 20800.00 10000.00 0.00 0.04636 21000.00 10000.00 0.00 0.04667 21200.00 10000.00 0.00 0.04690 20800.00 10200.00 0.00 0.04706 21000.00 10200.00 0.00 0.04732 21200.00 10200.00 0.00 0.04751 20800.00 10400.00 0.00 0.04756 21000.00 10400.00 0.00 0.04779 21200.00 10400.00 0.00 0.04794 20800.00 10600.00 0.00 0.04818 21000.00 10600.00 0.00 0.04841 21200.00 10600.00 0.00 0.04857 20800.00 10800.00 0.00 0.04902 21000.00 10800.00 0.00 0.04930 21200.00 10800.00 0.00 0.04949 20800.00 11000.00 0.00 0.05005 21000.00 11000.00 0.00 0.05035 21200.00 11000.00 0.00 0.05053 20800.00 11200.00 0.00 0.05111 21000.00 11200.00 0.00 0.05138 21200.00 11200.00 0.00 0.05152 20600.00 11400.00 0.00 0.05156 20800.00 11400.00 0.00 0.05223 21000.00 11400.00 0.00 0.05247 21200.00 11400.00 0.00 0.05261 20800.00 11600.00 0.00 0.05319 21000.00 11600.00 0.00 0.05343 21200.00 11600.00 0.00 0.05360 20800.00 11800.00 0.00 0.05401 21000.00 11800.00 0.00 0.05428 21200.00 11800.00 0.00 0.05447 20800.00 12000.00 0.00 0.05495 21000.00 12000.00 0.00 0.05526 21200.00 12000.00 0.00 0.05543 20800.00 12200.00 0.00 0.05604 21000.00 12200.00 0.00 0.05635 21200.00 12200.00 0.00 0.05645 20800.00 12400.00 0.00 0.05718 21000.00 12400.00 0.00 0.05742 21200.00 12400.00 0.00 0.05745 20600.00 12600.00 0.00 0.05751 20800.00 12600.00 0.00 0.05822 21000.00 12600.00 0.00 0.05841 20600.00 12800.00 0.00 0.05869 20800.00 12800.00 0.00 0.05939 21000.00 12800.00 0.00 0.05952 20600.00 13000.00 0.00 0.05985 20800.00 13000.00 0.00 0.06051 21000.00 13000.00 0.00 0.06059 20600.00 13200.00 0.00 0.06100 20800.00 13200.00 0.00 0.06161 21000.00 13200.00 0.00 0.06166 20600.00 13400.00 0.00 0.06225 20800.00 13400.00 0.00 0.06281 21000.00 13400.00 0.00 0.06287 21200.00 13400.00 0.00 0.06288 20600.00 13600.00 0.00 0.06347 20800.00 13600.00 0.00 0.06399 21000.00 13600.00 0.00 0.06405 21200.00 13600.00 0.00 0.06407 20600.00 13800.00 0.00 0.06464 20800.00 13800.00 0.00 0.06514 21000.00 13800.00 0.00 0.06520 20600.00 14000.00 0.00 0.06576 20800.00 14000.00 0.00 0.06626 21000.00 14000.00 0.00 0.06632 20600.00 14200.00 0.00 0.06700 20800.00 14200.00 0.00 0.06751 21000.00 14200.00 0.00 0.06756 20600.00 14400.00 0.00 0.06831 20800.00 14400.00 0.00 0.06882 21000.00 14400.00 0.00 0.06884 20600.00 14600.00 0.00 0.06956 20800.00 14600.00 0.00 0.07007 21000.00 14600.00 0.00 0.07009 21200.00 14600.00 0.00 0.07010 20600.00 14800.00 0.00 0.07076 20800.00 14800.00 0.00 0.07130 21000.00 14800.00 0.00 0.07138 21200.00 14800.00 0.00 0.07145 20600.00 15000.00 0.00 0.07222 20800.00 15000.00 0.00 0.07281 21000.00 15000.00 0.00 0.07294 21200.00 15000.00 0.00 0.07300 20600.00 15200.00 0.00 0.07398 20800.00 15200.00 0.00 0.07458 21000.00 15200.00 0.00 0.07471 20600.00 15400.00 0.00 0.07592 20800.00 15400.00 0.00 0.07649 21000.00 15400.00 0.00 0.07656 20600.00 15600.00 0.00 0.07785 20800.00 15600.00 0.00 0.07836 21000.00 15600.00 0.00 0.07839 20600.00 15800.00 0.00 0.07957 20800.00 15800.00 0.00 0.08005 20600.00 16000.00 0.00 0.08138 20800.00 16000.00 0.00 0.08183 20600.00 16200.00 0.00 0.08328 20800.00 16200.00 0.00 0.08374 20600.00 16400.00 0.00 0.08530 20800.00 16400.00 0.00 0.08576 20600.00 16600.00 0.00 0.08735 20800.00 16600.00 0.00 0.08781 20400.00 16800.00 0.00 0.08785 20600.00 16800.00 0.00 0.08952 20800.00 16800.00 0.00 0.08993 20400.00 17000.00 0.00 0.09003 20600.00 17000.00 0.00 0.09169 20800.00 17000.00 0.00 0.09205 20400.00 17200.00 0.00 0.09230 20600.00 17200.00 0.00 0.09394 20800.00 17200.00 0.00 0.09425 20400.00 17400.00 0.00 0.09474 20600.00 17400.00 0.00 0.09634 20800.00 17400.00 0.00 0.09659 20400.00 17600.00 0.00 0.09692 20600.00 17600.00 0.00 0.09845 20800.00 17600.00 0.00 0.09862 20400.00 17800.00 0.00 0.09917 20600.00 17800.00 0.00 0.10068 20800.00 17800.00 0.00 0.10079 20400.00 18000.00 0.00 0.10152 20600.00 18000.00 0.00 0.10304 20800.00 18000.00 0.00 0.10312 20400.00 18200.00 0.00 0.10404 20600.00 18200.00 0.00 0.10553 20400.00 18400.00 0.00 0.10669 20600.00 18400.00 0.00 0.10814 20400.00 18600.00 0.00 0.10957 20600.00 18600.00 0.00 0.11096 20400.00 18800.00 0.00 0.11275 20600.00 18800.00 0.00 0.11407 20400.00 19000.00 0.00 0.11594 20600.00 19000.00 0.00 0.11714 20400.00 19200.00 0.00 0.11936 20600.00 19200.00 0.00 0.12040 20400.00 19400.00 0.00 0.12271 20600.00 19400.00 0.00 0.12355 20400.00 19600.00 0.00 0.12625 20600.00 19600.00 0.00 0.12678 20200.00 19800.00 0.00 0.12692 20400.00 19800.00 0.00 0.12946 20600.00 19800.00 0.00 0.12961 20200.00 20000.00 0.00 0.13022 20400.00 20000.00 0.00 0.13249 20200.00 20200.00 0.00 0.13382 20400.00 20200.00 0.00 0.13547 20200.00 20400.00 0.00 0.13605 20400.00 20400.00 0.00 0.13696 20200.00 20600.00 15.00 0.13703 20200.00 20600.00 20.00 0.13710 20200.00 20600.00 25.00 0.13712 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20295.50 20523.80 22.86 0.13750 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/428.ctf NX, NY, NZ: 512 512 1 MODE : real