CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/427.mrc Output diagnostic file name Micrographs_0601/427.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/427.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2360.000 8340.000 Mean, RMS : 4475.792 216.9056 TITLE 1: emi2mrc: 427.emi Mon Jan 06 14:31:19 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.06%, Obj 88.06%, Dif 73.67% TITLE 3: X 294.461, Y 848.287, Z -24.919, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01824 11600.00 10000.00 0.00 -0.01794 11800.00 10000.00 0.00 -0.01787 12200.00 10000.00 0.00 -0.01775 12400.00 10000.00 0.00 -0.01765 12600.00 10000.00 0.00 -0.01757 12800.00 10000.00 0.00 -0.01739 13000.00 10000.00 0.00 -0.01720 13200.00 10000.00 0.00 -0.01706 13400.00 10000.00 0.00 -0.01684 13600.00 10000.00 0.00 -0.01641 13800.00 10000.00 0.00 -0.01587 14000.00 10000.00 0.00 -0.01532 14200.00 10000.00 0.00 -0.01491 14400.00 10000.00 0.00 -0.01442 14600.00 10000.00 0.00 -0.01376 14800.00 10000.00 0.00 -0.01321 15000.00 10000.00 0.00 -0.01265 15200.00 10000.00 0.00 -0.01191 15400.00 10000.00 0.00 -0.01114 15600.00 10000.00 0.00 -0.01036 15800.00 10000.00 0.00 -0.00954 16000.00 10000.00 0.00 -0.00874 16200.00 10000.00 0.00 -0.00789 16400.00 10000.00 0.00 -0.00693 16600.00 10000.00 0.00 -0.00598 16800.00 10000.00 0.00 -0.00505 17000.00 10000.00 0.00 -0.00400 17200.00 10000.00 0.00 -0.00300 17400.00 10000.00 0.00 -0.00212 17600.00 10000.00 0.00 -0.00108 17800.00 10000.00 0.00 -0.00006 18000.00 10000.00 0.00 0.00087 18200.00 10000.00 0.00 0.00203 18400.00 10000.00 0.00 0.00327 18600.00 10000.00 0.00 0.00447 18800.00 10000.00 0.00 0.00572 19000.00 10000.00 0.00 0.00699 19200.00 10000.00 0.00 0.00825 19400.00 10000.00 0.00 0.00946 19600.00 10000.00 0.00 0.01063 19800.00 10000.00 0.00 0.01189 20000.00 10000.00 0.00 0.01321 20200.00 10000.00 0.00 0.01470 20400.00 10000.00 0.00 0.01619 20600.00 10000.00 0.00 0.01767 20800.00 10000.00 0.00 0.01933 21000.00 10000.00 0.00 0.02106 21200.00 10000.00 0.00 0.02286 21400.00 10000.00 0.00 0.02478 21600.00 10000.00 0.00 0.02667 21800.00 10000.00 0.00 0.02864 22000.00 10000.00 0.00 0.03093 22200.00 10000.00 0.00 0.03322 22400.00 10000.00 0.00 0.03502 22600.00 10000.00 0.00 0.03613 22800.00 10000.00 0.00 0.03670 23000.00 10000.00 0.00 0.03688 22800.00 10200.00 0.00 0.03723 23000.00 10200.00 0.00 0.03743 22800.00 10400.00 0.00 0.03780 23000.00 10400.00 0.00 0.03802 22600.00 10600.00 0.00 0.03804 22800.00 10600.00 0.00 0.03856 23000.00 10600.00 0.00 0.03876 22600.00 10800.00 0.00 0.03887 22800.00 10800.00 0.00 0.03936 23000.00 10800.00 0.00 0.03953 22600.00 11000.00 0.00 0.03968 22800.00 11000.00 0.00 0.04012 23000.00 11000.00 0.00 0.04028 22600.00 11200.00 0.00 0.04053 22800.00 11200.00 0.00 0.04093 23000.00 11200.00 0.00 0.04108 22600.00 11400.00 0.00 0.04140 22800.00 11400.00 0.00 0.04177 23000.00 11400.00 0.00 0.04191 22600.00 11600.00 0.00 0.04214 22800.00 11600.00 0.00 0.04250 23000.00 11600.00 0.00 0.04265 22600.00 11800.00 0.00 0.04278 22800.00 11800.00 0.00 0.04317 23000.00 11800.00 0.00 0.04332 22600.00 12000.00 0.00 0.04351 22800.00 12000.00 0.00 0.04390 23000.00 12000.00 0.00 0.04405 22600.00 12200.00 0.00 0.04423 22800.00 12200.00 0.00 0.04464 23000.00 12200.00 0.00 0.04481 22600.00 12400.00 0.00 0.04500 22800.00 12400.00 0.00 0.04546 23000.00 12400.00 0.00 0.04566 22600.00 12600.00 0.00 0.04600 22800.00 12600.00 0.00 0.04648 23000.00 12600.00 0.00 0.04662 22600.00 12800.00 0.00 0.04692 22800.00 12800.00 0.00 0.04734 23000.00 12800.00 0.00 0.04738 22600.00 13000.00 0.00 0.04782 22800.00 13000.00 0.00 0.04818 22600.00 13200.00 0.00 0.04889 22800.00 13200.00 0.00 0.04916 22600.00 13400.00 0.00 0.04969 22800.00 13400.00 0.00 0.04990 22600.00 13600.00 0.00 0.05042 22800.00 13600.00 0.00 0.05065 22600.00 13800.00 0.00 0.05124 22800.00 13800.00 0.00 0.05150 23000.00 13800.00 0.00 0.05151 22600.00 14000.00 0.00 0.05211 22800.00 14000.00 0.00 0.05242 23000.00 14000.00 0.00 0.05244 22600.00 14200.00 0.00 0.05306 22800.00 14200.00 0.00 0.05341 22600.00 14400.00 0.00 0.05409 22800.00 14400.00 0.00 0.05440 22400.00 14600.00 0.00 0.05452 22600.00 14600.00 0.00 0.05527 22800.00 14600.00 0.00 0.05554 22400.00 14800.00 0.00 0.05573 22600.00 14800.00 0.00 0.05646 22800.00 14800.00 0.00 0.05678 22400.00 15000.00 0.00 0.05699 22600.00 15000.00 0.00 0.05777 22800.00 15000.00 0.00 0.05810 22400.00 15200.00 0.00 0.05813 22600.00 15200.00 0.00 0.05893 22800.00 15200.00 0.00 0.05921 22400.00 15400.00 0.00 0.05931 22600.00 15400.00 0.00 0.06012 22800.00 15400.00 0.00 0.06037 22400.00 15600.00 0.00 0.06074 22600.00 15600.00 0.00 0.06153 22800.00 15600.00 0.00 0.06173 22400.00 15800.00 0.00 0.06194 22600.00 15800.00 0.00 0.06267 22800.00 15800.00 0.00 0.06279 22400.00 16000.00 0.00 0.06311 22600.00 16000.00 0.00 0.06382 22800.00 16000.00 0.00 0.06391 22400.00 16200.00 0.00 0.06444 22600.00 16200.00 0.00 0.06515 22800.00 16200.00 0.00 0.06521 22400.00 16400.00 0.00 0.06574 22600.00 16400.00 0.00 0.06640 22400.00 16600.00 0.00 0.06687 22600.00 16600.00 0.00 0.06749 22400.00 16800.00 0.00 0.06817 22600.00 16800.00 0.00 0.06877 22400.00 17000.00 0.00 0.06963 22600.00 17000.00 0.00 0.07026 22400.00 17200.00 0.00 0.07104 22600.00 17200.00 0.00 0.07170 22400.00 17400.00 0.00 0.07257 22600.00 17400.00 0.00 0.07318 22400.00 17600.00 0.00 0.07405 22600.00 17600.00 0.00 0.07458 22400.00 17800.00 0.00 0.07557 22600.00 17800.00 0.00 0.07608 22400.00 18000.00 0.00 0.07723 22600.00 18000.00 0.00 0.07772 22400.00 18200.00 0.00 0.07905 22600.00 18200.00 0.00 0.07949 22400.00 18400.00 0.00 0.08081 22600.00 18400.00 0.00 0.08119 22400.00 18600.00 0.00 0.08253 22600.00 18600.00 0.00 0.08288 22400.00 18800.00 0.00 0.08417 22600.00 18800.00 0.00 0.08455 22400.00 19000.00 0.00 0.08563 22600.00 19000.00 0.00 0.08601 22400.00 19200.00 0.00 0.08735 22600.00 19200.00 0.00 0.08766 22400.00 19400.00 0.00 0.08901 22600.00 19400.00 0.00 0.08924 22200.00 19600.00 0.00 0.08925 22400.00 19600.00 0.00 0.09078 22600.00 19600.00 0.00 0.09094 22200.00 19800.00 0.00 0.09126 22400.00 19800.00 0.00 0.09276 22600.00 19800.00 0.00 0.09282 22200.00 20000.00 0.00 0.09321 22400.00 20000.00 0.00 0.09464 22200.00 20200.00 0.00 0.09488 22400.00 20200.00 0.00 0.09621 22200.00 20400.00 0.00 0.09640 22400.00 20400.00 0.00 0.09764 22200.00 20600.00 0.00 0.09821 22400.00 20600.00 0.00 0.09936 22200.00 20800.00 0.00 0.09990 22400.00 20800.00 0.00 0.10097 22200.00 21000.00 0.00 0.10146 22400.00 21000.00 0.00 0.10245 22200.00 21200.00 0.00 0.10286 22400.00 21200.00 0.00 0.10377 22200.00 21400.00 0.00 0.10438 22400.00 21400.00 0.00 0.10514 22200.00 21600.00 0.00 0.10587 22400.00 21600.00 0.00 0.10647 22200.00 21800.00 0.00 0.10725 22400.00 21800.00 0.00 0.10765 22200.00 22000.00 0.00 0.10873 22400.00 22000.00 0.00 0.10887 22200.00 22200.00 0.00 0.10984 22200.00 22400.00 0.00 0.11069 22200.00 22400.00 5.00 0.11076 22200.00 22400.00 10.00 0.11081 22200.00 22400.00 15.00 0.11084 22200.00 22400.00 20.00 0.11086 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22217.04 22429.71 17.55 0.11089 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/427.ctf NX, NY, NZ: 512 512 1 MODE : real