CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/423.mrc Output diagnostic file name Micrographs_0601/423.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/423.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1646.000 8284.000 Mean, RMS : 4560.716 226.6196 TITLE 1: emi2mrc: 423.emi Mon Jan 06 14:21:36 2014 TITLE 2: kV 300, Mag 78000x, Def 21660, Spot 4, Int 55.05%, Obj 88.05%, Dif 73.67% TITLE 3: X 297.987, Y 848.778, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02295 15000.00 10000.00 0.00 -0.02282 15200.00 10000.00 0.00 -0.02260 15400.00 10000.00 0.00 -0.02225 15600.00 10000.00 0.00 -0.02189 15800.00 10000.00 0.00 -0.02145 16000.00 10000.00 0.00 -0.02100 16200.00 10000.00 0.00 -0.02062 16400.00 10000.00 0.00 -0.02015 16600.00 10000.00 0.00 -0.01963 16800.00 10000.00 0.00 -0.01905 17000.00 10000.00 0.00 -0.01838 17200.00 10000.00 0.00 -0.01765 17400.00 10000.00 0.00 -0.01696 17600.00 10000.00 0.00 -0.01625 17800.00 10000.00 0.00 -0.01547 18000.00 10000.00 0.00 -0.01460 18200.00 10000.00 0.00 -0.01376 18400.00 10000.00 0.00 -0.01297 18600.00 10000.00 0.00 -0.01197 18800.00 10000.00 0.00 -0.01084 19000.00 10000.00 0.00 -0.00983 19200.00 10000.00 0.00 -0.00879 19400.00 10000.00 0.00 -0.00782 19600.00 10000.00 0.00 -0.00698 19800.00 10000.00 0.00 -0.00609 20000.00 10000.00 0.00 -0.00534 20200.00 10000.00 0.00 -0.00457 20400.00 10000.00 0.00 -0.00367 20600.00 10000.00 0.00 -0.00276 20800.00 10000.00 0.00 -0.00174 21000.00 10000.00 0.00 -0.00072 21200.00 10000.00 0.00 0.00035 21400.00 10000.00 0.00 0.00162 21600.00 10000.00 0.00 0.00287 21800.00 10000.00 0.00 0.00399 22000.00 10000.00 0.00 0.00512 22200.00 10000.00 0.00 0.00639 22400.00 10000.00 0.00 0.00776 22600.00 10000.00 0.00 0.00927 22800.00 10000.00 0.00 0.01084 23000.00 10000.00 0.00 0.01252 23200.00 10000.00 0.00 0.01439 23400.00 10000.00 0.00 0.01640 23600.00 10000.00 0.00 0.01841 23800.00 10000.00 0.00 0.02049 24000.00 10000.00 0.00 0.02277 24200.00 10000.00 0.00 0.02485 24400.00 10000.00 0.00 0.02620 24600.00 10000.00 0.00 0.02683 24800.00 10000.00 0.00 0.02702 25000.00 10000.00 0.00 0.02706 25200.00 10000.00 0.00 0.02709 24600.00 10200.00 0.00 0.02717 24800.00 10200.00 0.00 0.02735 25000.00 10200.00 0.00 0.02739 25200.00 10200.00 0.00 0.02743 24600.00 10400.00 0.00 0.02754 24800.00 10400.00 0.00 0.02770 25000.00 10400.00 0.00 0.02775 25200.00 10400.00 0.00 0.02780 24600.00 10600.00 0.00 0.02799 24800.00 10600.00 0.00 0.02812 25000.00 10600.00 0.00 0.02817 25200.00 10600.00 0.00 0.02820 24600.00 10800.00 0.00 0.02837 24800.00 10800.00 0.00 0.02847 25000.00 10800.00 0.00 0.02854 24600.00 11000.00 0.00 0.02874 24800.00 11000.00 0.00 0.02886 25000.00 11000.00 0.00 0.02891 24600.00 11200.00 0.00 0.02916 24800.00 11200.00 0.00 0.02928 25000.00 11200.00 0.00 0.02929 24600.00 11400.00 0.00 0.02955 24800.00 11400.00 0.00 0.02966 24600.00 11600.00 0.00 0.02989 24800.00 11600.00 0.00 0.03001 24600.00 11800.00 0.00 0.03033 24800.00 11800.00 0.00 0.03043 24600.00 12000.00 0.00 0.03082 24800.00 12000.00 0.00 0.03088 24600.00 12200.00 0.00 0.03124 24800.00 12200.00 0.00 0.03128 24600.00 12400.00 0.00 0.03163 24800.00 12400.00 0.00 0.03168 25000.00 12400.00 0.00 0.03170 24600.00 12600.00 0.00 0.03215 24800.00 12600.00 0.00 0.03223 25000.00 12600.00 0.00 0.03226 24600.00 12800.00 0.00 0.03277 24800.00 12800.00 0.00 0.03284 25000.00 12800.00 0.00 0.03287 24600.00 13000.00 0.00 0.03330 24800.00 13000.00 0.00 0.03339 25000.00 13000.00 0.00 0.03345 24400.00 13200.00 0.00 0.03351 24600.00 13200.00 0.00 0.03403 24800.00 13200.00 0.00 0.03415 25000.00 13200.00 0.00 0.03420 24400.00 13400.00 0.00 0.03426 24600.00 13400.00 0.00 0.03477 24800.00 13400.00 0.00 0.03487 25000.00 13400.00 0.00 0.03489 24400.00 13600.00 0.00 0.03496 24600.00 13600.00 0.00 0.03546 24800.00 13600.00 0.00 0.03555 25000.00 13600.00 0.00 0.03558 24400.00 13800.00 0.00 0.03580 24600.00 13800.00 0.00 0.03629 24800.00 13800.00 0.00 0.03638 25000.00 13800.00 0.00 0.03640 24400.00 14000.00 0.00 0.03660 24600.00 14000.00 0.00 0.03706 24800.00 14000.00 0.00 0.03713 24400.00 14200.00 0.00 0.03742 24600.00 14200.00 0.00 0.03783 24800.00 14200.00 0.00 0.03789 25000.00 14200.00 0.00 0.03790 24400.00 14400.00 0.00 0.03811 24600.00 14400.00 0.00 0.03852 24800.00 14400.00 0.00 0.03863 25000.00 14400.00 0.00 0.03866 24400.00 14600.00 0.00 0.03880 24600.00 14600.00 0.00 0.03925 24800.00 14600.00 0.00 0.03941 25000.00 14600.00 0.00 0.03942 24400.00 14800.00 0.00 0.03963 24600.00 14800.00 0.00 0.04013 24800.00 14800.00 0.00 0.04030 24400.00 15000.00 0.00 0.04051 24600.00 15000.00 0.00 0.04101 24800.00 15000.00 0.00 0.04115 24400.00 15200.00 0.00 0.04144 24600.00 15200.00 0.00 0.04194 24800.00 15200.00 0.00 0.04204 24400.00 15400.00 0.00 0.04239 24600.00 15400.00 0.00 0.04287 24800.00 15400.00 0.00 0.04295 24400.00 15600.00 0.00 0.04331 24600.00 15600.00 0.00 0.04380 24800.00 15600.00 0.00 0.04386 24400.00 15800.00 0.00 0.04428 24600.00 15800.00 0.00 0.04479 24800.00 15800.00 0.00 0.04482 24400.00 16000.00 0.00 0.04533 24600.00 16000.00 0.00 0.04583 24400.00 16200.00 0.00 0.04637 24600.00 16200.00 0.00 0.04685 24400.00 16400.00 0.00 0.04748 24600.00 16400.00 0.00 0.04797 24800.00 16400.00 0.00 0.04799 24400.00 16600.00 0.00 0.04861 24600.00 16600.00 0.00 0.04914 24800.00 16600.00 0.00 0.04920 24400.00 16800.00 0.00 0.04979 24600.00 16800.00 0.00 0.05032 24800.00 16800.00 0.00 0.05038 24400.00 17000.00 0.00 0.05104 24600.00 17000.00 0.00 0.05157 24800.00 17000.00 0.00 0.05161 24400.00 17200.00 0.00 0.05233 24600.00 17200.00 0.00 0.05285 24400.00 17400.00 0.00 0.05369 24600.00 17400.00 0.00 0.05415 24400.00 17600.00 0.00 0.05495 24600.00 17600.00 0.00 0.05534 24400.00 17800.00 0.00 0.05620 24600.00 17800.00 0.00 0.05655 24400.00 18000.00 0.00 0.05747 24600.00 18000.00 0.00 0.05779 24400.00 18200.00 0.00 0.05878 24600.00 18200.00 0.00 0.05910 24400.00 18400.00 0.00 0.06014 24600.00 18400.00 0.00 0.06048 24400.00 18600.00 0.00 0.06161 24600.00 18600.00 0.00 0.06197 24400.00 18800.00 0.00 0.06310 24600.00 18800.00 0.00 0.06347 24400.00 19000.00 0.00 0.06451 24600.00 19000.00 0.00 0.06488 24400.00 19200.00 0.00 0.06604 24600.00 19200.00 0.00 0.06637 24400.00 19400.00 0.00 0.06743 24600.00 19400.00 0.00 0.06772 24400.00 19600.00 0.00 0.06879 24600.00 19600.00 0.00 0.06909 24400.00 19800.00 0.00 0.07026 24600.00 19800.00 0.00 0.07058 24400.00 20000.00 0.00 0.07182 24600.00 20000.00 0.00 0.07212 24400.00 20200.00 0.00 0.07342 24600.00 20200.00 0.00 0.07365 24400.00 20400.00 0.00 0.07501 24600.00 20400.00 0.00 0.07517 24200.00 20600.00 0.00 0.07539 24400.00 20600.00 0.00 0.07679 24600.00 20600.00 0.00 0.07690 24200.00 20800.00 0.00 0.07708 24400.00 20800.00 0.00 0.07847 24600.00 20800.00 0.00 0.07854 24200.00 21000.00 0.00 0.07875 24400.00 21000.00 0.00 0.08014 24600.00 21000.00 0.00 0.08019 24200.00 21200.00 0.00 0.08047 24400.00 21200.00 0.00 0.08188 24600.00 21200.00 0.00 0.08190 24200.00 21400.00 0.00 0.08231 24400.00 21400.00 0.00 0.08374 24200.00 21600.00 0.00 0.08436 24400.00 21600.00 0.00 0.08580 24200.00 21800.00 0.00 0.08656 24400.00 21800.00 0.00 0.08790 24200.00 22000.00 0.00 0.08876 24400.00 22000.00 0.00 0.08999 24200.00 22200.00 0.00 0.09086 24400.00 22200.00 0.00 0.09203 24200.00 22400.00 0.00 0.09317 24400.00 22400.00 0.00 0.09430 24200.00 22600.00 0.00 0.09560 24400.00 22600.00 0.00 0.09658 24200.00 22800.00 0.00 0.09799 24400.00 22800.00 0.00 0.09881 24200.00 23000.00 0.00 0.10024 24400.00 23000.00 0.00 0.10093 24200.00 23200.00 0.00 0.10264 24400.00 23200.00 0.00 0.10320 24200.00 23400.00 0.00 0.10512 24400.00 23400.00 0.00 0.10546 24200.00 23600.00 0.00 0.10743 24400.00 23600.00 0.00 0.10749 24000.00 23800.00 0.00 0.10794 24200.00 23800.00 0.00 0.10986 24000.00 24000.00 0.00 0.11025 24200.00 24000.00 0.00 0.11191 24000.00 24200.00 0.00 0.11214 24200.00 24200.00 0.00 0.11338 24000.00 24400.00 25.00 0.11346 24000.00 24400.00 30.00 0.11354 24000.00 24400.00 35.00 0.11358 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24112.04 24330.73 33.05 0.11392 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/423.ctf NX, NY, NZ: 512 512 1 MODE : real