CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/422.mrc Output diagnostic file name Micrographs_0601/422.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/422.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2322.000 9040.000 Mean, RMS : 4496.201 224.6159 TITLE 1: emi2mrc: 422.emi Mon Jan 06 14:24:13 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 294.388, Y 852.606, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02442 15600.00 10000.00 0.00 -0.02442 15800.00 10000.00 0.00 -0.02410 16000.00 10000.00 0.00 -0.02367 16200.00 10000.00 0.00 -0.02318 16400.00 10000.00 0.00 -0.02272 16600.00 10000.00 0.00 -0.02225 16800.00 10000.00 0.00 -0.02158 17000.00 10000.00 0.00 -0.02089 17200.00 10000.00 0.00 -0.02036 17400.00 10000.00 0.00 -0.01974 17600.00 10000.00 0.00 -0.01895 17800.00 10000.00 0.00 -0.01810 18000.00 10000.00 0.00 -0.01723 18200.00 10000.00 0.00 -0.01636 18400.00 10000.00 0.00 -0.01546 18600.00 10000.00 0.00 -0.01450 18800.00 10000.00 0.00 -0.01366 19000.00 10000.00 0.00 -0.01294 19200.00 10000.00 0.00 -0.01209 19400.00 10000.00 0.00 -0.01127 19600.00 10000.00 0.00 -0.01057 19800.00 10000.00 0.00 -0.00970 20000.00 10000.00 0.00 -0.00883 20200.00 10000.00 0.00 -0.00804 20400.00 10000.00 0.00 -0.00724 20600.00 10000.00 0.00 -0.00634 20800.00 10000.00 0.00 -0.00533 21000.00 10000.00 0.00 -0.00444 21200.00 10000.00 0.00 -0.00356 21400.00 10000.00 0.00 -0.00257 21600.00 10000.00 0.00 -0.00154 21800.00 10000.00 0.00 -0.00072 22000.00 10000.00 0.00 -0.00004 22200.00 10000.00 0.00 0.00087 22400.00 10000.00 0.00 0.00186 22600.00 10000.00 0.00 0.00286 22800.00 10000.00 0.00 0.00412 23000.00 10000.00 0.00 0.00561 23200.00 10000.00 0.00 0.00713 23400.00 10000.00 0.00 0.00861 23600.00 10000.00 0.00 0.01023 23800.00 10000.00 0.00 0.01208 24000.00 10000.00 0.00 0.01396 24200.00 10000.00 0.00 0.01577 24400.00 10000.00 0.00 0.01773 24600.00 10000.00 0.00 0.01983 24800.00 10000.00 0.00 0.02174 25000.00 10000.00 0.00 0.02295 25200.00 10000.00 0.00 0.02351 25400.00 10000.00 0.00 0.02384 25200.00 10200.00 0.00 0.02390 25400.00 10200.00 0.00 0.02415 25400.00 10400.00 0.00 0.02438 25200.00 10600.00 0.00 0.02447 25400.00 10600.00 0.00 0.02470 25200.00 10800.00 0.00 0.02489 25400.00 10800.00 0.00 0.02509 25200.00 11000.00 0.00 0.02533 25400.00 11000.00 0.00 0.02550 25200.00 11200.00 0.00 0.02578 25400.00 11200.00 0.00 0.02592 25200.00 11400.00 0.00 0.02624 25400.00 11400.00 0.00 0.02636 25200.00 11600.00 0.00 0.02671 25400.00 11600.00 0.00 0.02681 25000.00 11800.00 0.00 0.02684 25200.00 11800.00 0.00 0.02725 25400.00 11800.00 0.00 0.02735 25000.00 12000.00 0.00 0.02751 25200.00 12000.00 0.00 0.02791 25400.00 12000.00 0.00 0.02803 25000.00 12200.00 0.00 0.02825 25200.00 12200.00 0.00 0.02864 25400.00 12200.00 0.00 0.02876 25000.00 12400.00 0.00 0.02887 25200.00 12400.00 0.00 0.02924 25400.00 12400.00 0.00 0.02938 25000.00 12600.00 0.00 0.02940 25200.00 12600.00 0.00 0.02979 25400.00 12600.00 0.00 0.02995 25200.00 12800.00 0.00 0.03031 25400.00 12800.00 0.00 0.03046 25200.00 13000.00 0.00 0.03073 25400.00 13000.00 0.00 0.03083 25000.00 13200.00 0.00 0.03084 25200.00 13200.00 0.00 0.03125 25400.00 13200.00 0.00 0.03132 25200.00 13400.00 0.00 0.03170 25400.00 13400.00 0.00 0.03178 25200.00 13600.00 0.00 0.03210 25400.00 13600.00 0.00 0.03221 25000.00 13800.00 0.00 0.03223 25200.00 13800.00 0.00 0.03271 25400.00 13800.00 0.00 0.03281 25000.00 14000.00 0.00 0.03304 25200.00 14000.00 0.00 0.03350 25400.00 14000.00 0.00 0.03355 25000.00 14200.00 0.00 0.03386 25200.00 14200.00 0.00 0.03429 25400.00 14200.00 0.00 0.03430 25000.00 14400.00 0.00 0.03457 25200.00 14400.00 0.00 0.03499 25000.00 14600.00 0.00 0.03535 25200.00 14600.00 0.00 0.03578 25000.00 14800.00 0.00 0.03617 25200.00 14800.00 0.00 0.03656 25000.00 15000.00 0.00 0.03686 25200.00 15000.00 0.00 0.03719 25000.00 15200.00 0.00 0.03748 25200.00 15200.00 0.00 0.03780 25000.00 15400.00 0.00 0.03832 25200.00 15400.00 0.00 0.03864 25400.00 15400.00 0.00 0.03865 25000.00 15600.00 0.00 0.03924 25200.00 15600.00 0.00 0.03957 25400.00 15600.00 0.00 0.03957 25000.00 15800.00 0.00 0.04016 25200.00 15800.00 0.00 0.04049 25000.00 16000.00 0.00 0.04119 25200.00 16000.00 0.00 0.04152 25000.00 16200.00 0.00 0.04224 25200.00 16200.00 0.00 0.04258 25000.00 16400.00 0.00 0.04343 25200.00 16400.00 0.00 0.04377 25000.00 16600.00 0.00 0.04466 25200.00 16600.00 0.00 0.04498 25000.00 16800.00 0.00 0.04591 25200.00 16800.00 0.00 0.04617 25000.00 17000.00 0.00 0.04707 25200.00 17000.00 0.00 0.04725 25000.00 17200.00 0.00 0.04807 25200.00 17200.00 0.00 0.04825 25000.00 17400.00 0.00 0.04913 25200.00 17400.00 0.00 0.04933 25000.00 17600.00 0.00 0.05015 25200.00 17600.00 0.00 0.05033 25000.00 17800.00 0.00 0.05123 25200.00 17800.00 0.00 0.05141 24800.00 18000.00 0.00 0.05142 25000.00 18000.00 0.00 0.05241 25200.00 18000.00 0.00 0.05261 25000.00 18200.00 0.00 0.05361 25200.00 18200.00 0.00 0.05381 25000.00 18400.00 0.00 0.05485 25200.00 18400.00 0.00 0.05504 24800.00 18600.00 0.00 0.05504 25000.00 18600.00 0.00 0.05611 25200.00 18600.00 0.00 0.05630 24800.00 18800.00 0.00 0.05634 25000.00 18800.00 0.00 0.05743 25200.00 18800.00 0.00 0.05764 24800.00 19000.00 0.00 0.05773 25000.00 19000.00 0.00 0.05883 25200.00 19000.00 0.00 0.05903 24800.00 19200.00 0.00 0.05914 25000.00 19200.00 0.00 0.06023 25200.00 19200.00 0.00 0.06042 25000.00 19400.00 0.00 0.06153 25200.00 19400.00 0.00 0.06166 24800.00 19600.00 0.00 0.06171 25000.00 19600.00 0.00 0.06278 25200.00 19600.00 0.00 0.06280 24800.00 19800.00 0.00 0.06292 25000.00 19800.00 0.00 0.06391 24800.00 20000.00 0.00 0.06412 25000.00 20000.00 0.00 0.06506 24800.00 20200.00 0.00 0.06534 25000.00 20200.00 0.00 0.06630 24800.00 20400.00 0.00 0.06665 25000.00 20400.00 0.00 0.06760 24800.00 20600.00 0.00 0.06800 25000.00 20600.00 0.00 0.06891 24800.00 20800.00 0.00 0.06929 25000.00 20800.00 0.00 0.07015 24800.00 21000.00 0.00 0.07080 25000.00 21000.00 0.00 0.07164 24800.00 21200.00 0.00 0.07237 25000.00 21200.00 0.00 0.07321 24800.00 21400.00 0.00 0.07403 25000.00 21400.00 0.00 0.07488 24800.00 21600.00 0.00 0.07576 25000.00 21600.00 0.00 0.07659 24800.00 21800.00 0.00 0.07748 25000.00 21800.00 0.00 0.07827 24800.00 22000.00 0.00 0.07932 25000.00 22000.00 0.00 0.08010 24800.00 22200.00 0.00 0.08126 25000.00 22200.00 0.00 0.08207 24800.00 22400.00 0.00 0.08360 25000.00 22400.00 0.00 0.08439 24800.00 22600.00 0.00 0.08588 25000.00 22600.00 0.00 0.08657 24800.00 22800.00 0.00 0.08809 25000.00 22800.00 0.00 0.08867 24800.00 23000.00 0.00 0.09034 25000.00 23000.00 0.00 0.09085 24800.00 23200.00 0.00 0.09280 25000.00 23200.00 0.00 0.09325 24800.00 23400.00 0.00 0.09550 25000.00 23400.00 0.00 0.09583 24800.00 23600.00 0.00 0.09803 25000.00 23600.00 0.00 0.09820 24600.00 23800.00 0.00 0.09855 24800.00 23800.00 0.00 0.10079 24600.00 24000.00 0.00 0.10158 24800.00 24000.00 0.00 0.10373 24600.00 24200.00 0.00 0.10475 24800.00 24200.00 0.00 0.10669 24600.00 24400.00 0.00 0.10765 24800.00 24400.00 0.00 0.10932 24600.00 24600.00 0.00 0.11055 24800.00 24600.00 0.00 0.11180 24600.00 24800.00 0.00 0.11262 24800.00 24800.00 0.00 0.11309 24600.00 24800.00 20.00 0.11314 24600.00 24800.00 25.00 0.11320 24600.00 24800.00 30.00 0.11324 24600.00 24800.00 35.00 0.11324 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24669.46 24876.70 31.38 0.11380 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/422.ctf NX, NY, NZ: 512 512 1 MODE : real