CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/421.mrc Output diagnostic file name Micrographs_0601/421.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/421.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2416.000 8172.000 Mean, RMS : 4501.018 225.0783 TITLE 1: emi2mrc: 421.emi Mon Jan 06 14:23:05 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.06%, Obj 88.05%, Dif 73.67% TITLE 3: X 296.113, Y 850.717, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01991 13400.00 10000.00 0.00 -0.01978 13600.00 10000.00 0.00 -0.01948 13800.00 10000.00 0.00 -0.01928 14000.00 10000.00 0.00 -0.01911 14200.00 10000.00 0.00 -0.01887 14400.00 10000.00 0.00 -0.01861 14600.00 10000.00 0.00 -0.01820 14800.00 10000.00 0.00 -0.01774 15000.00 10000.00 0.00 -0.01740 15200.00 10000.00 0.00 -0.01715 15400.00 10000.00 0.00 -0.01686 15600.00 10000.00 0.00 -0.01652 15800.00 10000.00 0.00 -0.01620 16000.00 10000.00 0.00 -0.01572 16200.00 10000.00 0.00 -0.01497 16400.00 10000.00 0.00 -0.01412 16600.00 10000.00 0.00 -0.01331 16800.00 10000.00 0.00 -0.01254 17000.00 10000.00 0.00 -0.01187 17200.00 10000.00 0.00 -0.01123 17400.00 10000.00 0.00 -0.01046 17600.00 10000.00 0.00 -0.00976 17800.00 10000.00 0.00 -0.00920 18000.00 10000.00 0.00 -0.00848 18200.00 10000.00 0.00 -0.00759 18400.00 10000.00 0.00 -0.00670 18600.00 10000.00 0.00 -0.00591 18800.00 10000.00 0.00 -0.00504 19000.00 10000.00 0.00 -0.00415 19200.00 10000.00 0.00 -0.00317 19400.00 10000.00 0.00 -0.00202 19600.00 10000.00 0.00 -0.00101 19800.00 10000.00 0.00 0.00003 20000.00 10000.00 0.00 0.00116 20200.00 10000.00 0.00 0.00214 20400.00 10000.00 0.00 0.00318 20600.00 10000.00 0.00 0.00440 20800.00 10000.00 0.00 0.00560 21000.00 10000.00 0.00 0.00663 21200.00 10000.00 0.00 0.00770 21400.00 10000.00 0.00 0.00896 21600.00 10000.00 0.00 0.01023 21800.00 10000.00 0.00 0.01155 22000.00 10000.00 0.00 0.01298 22200.00 10000.00 0.00 0.01442 22400.00 10000.00 0.00 0.01596 22600.00 10000.00 0.00 0.01768 22800.00 10000.00 0.00 0.01953 23000.00 10000.00 0.00 0.02156 23200.00 10000.00 0.00 0.02348 23400.00 10000.00 0.00 0.02552 23600.00 10000.00 0.00 0.02736 23800.00 10000.00 0.00 0.02844 24000.00 10000.00 0.00 0.02910 24200.00 10000.00 0.00 0.02935 24000.00 10200.00 0.00 0.02954 24200.00 10200.00 0.00 0.02976 24000.00 10400.00 0.00 0.02993 24200.00 10400.00 0.00 0.03018 24000.00 10600.00 0.00 0.03038 24200.00 10600.00 0.00 0.03064 24400.00 10600.00 0.00 0.03065 24000.00 10800.00 0.00 0.03085 24200.00 10800.00 0.00 0.03110 24000.00 11000.00 0.00 0.03133 24200.00 11000.00 0.00 0.03157 24400.00 11000.00 0.00 0.03158 24000.00 11200.00 0.00 0.03188 24200.00 11200.00 0.00 0.03212 24400.00 11200.00 0.00 0.03216 24000.00 11400.00 0.00 0.03243 24200.00 11400.00 0.00 0.03266 24400.00 11400.00 0.00 0.03270 24000.00 11600.00 0.00 0.03283 24200.00 11600.00 0.00 0.03311 24400.00 11600.00 0.00 0.03314 24000.00 11800.00 0.00 0.03338 24200.00 11800.00 0.00 0.03368 24000.00 12000.00 0.00 0.03415 24200.00 12000.00 0.00 0.03439 24000.00 12200.00 0.00 0.03487 24200.00 12200.00 0.00 0.03505 24000.00 12400.00 0.00 0.03557 24200.00 12400.00 0.00 0.03573 24000.00 12600.00 0.00 0.03627 24200.00 12600.00 0.00 0.03640 24000.00 12800.00 0.00 0.03692 24200.00 12800.00 0.00 0.03704 24000.00 13000.00 0.00 0.03753 24200.00 13000.00 0.00 0.03767 23800.00 13200.00 0.00 0.03775 24000.00 13200.00 0.00 0.03831 24200.00 13200.00 0.00 0.03848 24400.00 13200.00 0.00 0.03848 23800.00 13400.00 0.00 0.03879 24000.00 13400.00 0.00 0.03933 24200.00 13400.00 0.00 0.03953 24400.00 13400.00 0.00 0.03955 23800.00 13600.00 0.00 0.03977 24000.00 13600.00 0.00 0.04028 24200.00 13600.00 0.00 0.04049 23800.00 13800.00 0.00 0.04065 24000.00 13800.00 0.00 0.04115 24200.00 13800.00 0.00 0.04136 23800.00 14000.00 0.00 0.04167 24000.00 14000.00 0.00 0.04218 24200.00 14000.00 0.00 0.04240 23800.00 14200.00 0.00 0.04263 24000.00 14200.00 0.00 0.04313 24200.00 14200.00 0.00 0.04330 23800.00 14400.00 0.00 0.04342 24000.00 14400.00 0.00 0.04389 24200.00 14400.00 0.00 0.04401 23800.00 14600.00 0.00 0.04428 24000.00 14600.00 0.00 0.04477 24200.00 14600.00 0.00 0.04488 23800.00 14800.00 0.00 0.04528 24000.00 14800.00 0.00 0.04581 24200.00 14800.00 0.00 0.04589 23800.00 15000.00 0.00 0.04639 24000.00 15000.00 0.00 0.04689 24200.00 15000.00 0.00 0.04689 23800.00 15200.00 0.00 0.04742 24000.00 15200.00 0.00 0.04786 23800.00 15400.00 0.00 0.04840 24000.00 15400.00 0.00 0.04879 23800.00 15600.00 0.00 0.04937 24000.00 15600.00 0.00 0.04975 23800.00 15800.00 0.00 0.05041 24000.00 15800.00 0.00 0.05079 23800.00 16000.00 0.00 0.05168 24000.00 16000.00 0.00 0.05198 23800.00 16200.00 0.00 0.05288 24000.00 16200.00 0.00 0.05311 23600.00 16400.00 0.00 0.05320 23800.00 16400.00 0.00 0.05407 24000.00 16400.00 0.00 0.05428 23600.00 16600.00 0.00 0.05436 23800.00 16600.00 0.00 0.05524 24000.00 16600.00 0.00 0.05547 23600.00 16800.00 0.00 0.05554 23800.00 16800.00 0.00 0.05642 24000.00 16800.00 0.00 0.05664 23600.00 17000.00 0.00 0.05676 23800.00 17000.00 0.00 0.05761 24000.00 17000.00 0.00 0.05779 23600.00 17200.00 0.00 0.05796 23800.00 17200.00 0.00 0.05877 24000.00 17200.00 0.00 0.05895 23600.00 17400.00 0.00 0.05923 23800.00 17400.00 0.00 0.06003 24000.00 17400.00 0.00 0.06027 23600.00 17600.00 0.00 0.06053 23800.00 17600.00 0.00 0.06137 24000.00 17600.00 0.00 0.06164 23600.00 17800.00 0.00 0.06202 23800.00 17800.00 0.00 0.06289 24000.00 17800.00 0.00 0.06319 23600.00 18000.00 0.00 0.06355 23800.00 18000.00 0.00 0.06444 24000.00 18000.00 0.00 0.06475 23600.00 18200.00 0.00 0.06503 23800.00 18200.00 0.00 0.06594 24000.00 18200.00 0.00 0.06624 23600.00 18400.00 0.00 0.06648 23800.00 18400.00 0.00 0.06738 24000.00 18400.00 0.00 0.06765 23600.00 18600.00 0.00 0.06794 23800.00 18600.00 0.00 0.06881 24000.00 18600.00 0.00 0.06910 23600.00 18800.00 0.00 0.06949 23800.00 18800.00 0.00 0.07040 24000.00 18800.00 0.00 0.07074 23600.00 19000.00 0.00 0.07103 23800.00 19000.00 0.00 0.07200 24000.00 19000.00 0.00 0.07237 23600.00 19200.00 0.00 0.07270 23800.00 19200.00 0.00 0.07373 24000.00 19200.00 0.00 0.07406 23600.00 19400.00 0.00 0.07447 23800.00 19400.00 0.00 0.07553 24000.00 19400.00 0.00 0.07580 23600.00 19600.00 0.00 0.07634 23800.00 19600.00 0.00 0.07738 24000.00 19600.00 0.00 0.07754 23600.00 19800.00 0.00 0.07810 23800.00 19800.00 0.00 0.07909 24000.00 19800.00 0.00 0.07917 23600.00 20000.00 0.00 0.07987 23800.00 20000.00 0.00 0.08083 24000.00 20000.00 0.00 0.08086 23600.00 20200.00 0.00 0.08178 23800.00 20200.00 0.00 0.08272 23600.00 20400.00 0.00 0.08371 23800.00 20400.00 0.00 0.08461 23600.00 20600.00 0.00 0.08579 23800.00 20600.00 0.00 0.08663 23600.00 20800.00 0.00 0.08784 23800.00 20800.00 0.00 0.08858 23600.00 21000.00 0.00 0.08996 23800.00 21000.00 0.00 0.09058 23600.00 21200.00 0.00 0.09210 23800.00 21200.00 0.00 0.09260 23600.00 21400.00 0.00 0.09435 23800.00 21400.00 0.00 0.09474 23600.00 21600.00 0.00 0.09658 23800.00 21600.00 0.00 0.09686 23600.00 21800.00 0.00 0.09880 23800.00 21800.00 0.00 0.09898 23400.00 22000.00 0.00 0.09938 23600.00 22000.00 0.00 0.10128 23800.00 22000.00 0.00 0.10134 23400.00 22200.00 0.00 0.10198 23600.00 22200.00 0.00 0.10378 23400.00 22400.00 0.00 0.10473 23600.00 22400.00 0.00 0.10637 23400.00 22600.00 0.00 0.10742 23600.00 22600.00 0.00 0.10891 23400.00 22800.00 0.00 0.11038 23600.00 22800.00 0.00 0.11168 23400.00 23000.00 0.00 0.11331 23600.00 23000.00 0.00 0.11433 23400.00 23200.00 0.00 0.11606 23600.00 23200.00 0.00 0.11666 23400.00 23400.00 0.00 0.11862 23400.00 23600.00 0.00 0.11956 23400.00 23600.00 5.00 0.11966 23400.00 23600.00 10.00 0.11975 23400.00 23600.00 15.00 0.11982 23400.00 23600.00 20.00 0.11987 23400.00 23600.00 25.00 0.11989 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23406.20 23687.41 25.00 0.12005 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/421.ctf NX, NY, NZ: 512 512 1 MODE : real